A novel MLST sequence type discovered in the first fatal case of Laribacter hongkongensis bacteremia clusters with the sequence types of other human isolates
Laribacter hongkongensis is a gram-negative, facultative anaerobic, motile, S-shaped, asaccharolytic, urease-positive bacillus in the Neisseriaceae family of β-proteobacteria. To date, all patients with L. hongkongensis infection have survived, including the two patients with L. hongkongensis bacter...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2014-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1038/emi.2014.39 |
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| author | Cindy WS Tse Shirly OT Curreem Ingrid Cheung Bone SF Tang Kit-Wah Leung Susanna KP Lau Patrick CY Woo |
| author_facet | Cindy WS Tse Shirly OT Curreem Ingrid Cheung Bone SF Tang Kit-Wah Leung Susanna KP Lau Patrick CY Woo |
| author_sort | Cindy WS Tse |
| collection | DOAJ |
| description | Laribacter hongkongensis is a gram-negative, facultative anaerobic, motile, S-shaped, asaccharolytic, urease-positive bacillus in the Neisseriaceae family of β-proteobacteria. To date, all patients with L. hongkongensis infection have survived, including the two patients with L. hongkongensis bacteremia and patients with L. hongkongensis gastroenteritis. In this study, we describe the clinical, microbiological and molecular characterization of the first fatal case associated with L. hongkongensis bacteremia in a patient with colonic carcinoma that metastasized to the liver. The identity of the isolate was confirmed via phenotypic tests and 16S rRNA gene sequencing. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI–TOF MS), using the Bruker database extended with L. hongkongensis reference strains, also identified the isolate as L. hongkongensis, with a top match score of 2.473. Multilocus sequence typing revealed a new sequence type (ST), and phylogenetic analysis and eBURST demonstrated unambiguously that the ST of the isolate was clustered with two other STs found exclusively in human patients, consistent with the theory that some clones of L. hongkongensis could be more virulent than others. Underlying liver diseases and ascites potentially represent distinct risk factors for invasive L. hongkongensis infection. More widespread use of MALDI–TOF MS for identification and improvements of selective media should facilitate the identification of more cases of L. hongkongensis infection. |
| first_indexed | 2024-03-11T23:20:32Z |
| format | Article |
| id | doaj.art-6895f383655c47169673a45930d33a6e |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-11T23:20:32Z |
| publishDate | 2014-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-6895f383655c47169673a45930d33a6e2023-09-20T14:20:33ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512014-01-01311710.1038/emi.2014.39A novel MLST sequence type discovered in the first fatal case of Laribacter hongkongensis bacteremia clusters with the sequence types of other human isolatesCindy WS Tse0Shirly OT Curreem1Ingrid Cheung2Bone SF Tang3Kit-Wah Leung4Susanna KP Lau5Patrick CY Woo6Department of Microbiology, Kwong Wah Hospital, Hong Kong, ChinaDepartment of Microbiology, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, Kwong Wah Hospital, Hong Kong, ChinaDepartment of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, ChinaDepartment of Microbiology, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, The University of Hong Kong, Hong Kong, ChinaLaribacter hongkongensis is a gram-negative, facultative anaerobic, motile, S-shaped, asaccharolytic, urease-positive bacillus in the Neisseriaceae family of β-proteobacteria. To date, all patients with L. hongkongensis infection have survived, including the two patients with L. hongkongensis bacteremia and patients with L. hongkongensis gastroenteritis. In this study, we describe the clinical, microbiological and molecular characterization of the first fatal case associated with L. hongkongensis bacteremia in a patient with colonic carcinoma that metastasized to the liver. The identity of the isolate was confirmed via phenotypic tests and 16S rRNA gene sequencing. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI–TOF MS), using the Bruker database extended with L. hongkongensis reference strains, also identified the isolate as L. hongkongensis, with a top match score of 2.473. Multilocus sequence typing revealed a new sequence type (ST), and phylogenetic analysis and eBURST demonstrated unambiguously that the ST of the isolate was clustered with two other STs found exclusively in human patients, consistent with the theory that some clones of L. hongkongensis could be more virulent than others. Underlying liver diseases and ascites potentially represent distinct risk factors for invasive L. hongkongensis infection. More widespread use of MALDI–TOF MS for identification and improvements of selective media should facilitate the identification of more cases of L. hongkongensis infection.https://www.tandfonline.com/doi/10.1038/emi.2014.39bacteraemiainfections |
| spellingShingle | Cindy WS Tse Shirly OT Curreem Ingrid Cheung Bone SF Tang Kit-Wah Leung Susanna KP Lau Patrick CY Woo A novel MLST sequence type discovered in the first fatal case of Laribacter hongkongensis bacteremia clusters with the sequence types of other human isolates Emerging Microbes and Infections bacteraemia infections |
| title | A novel MLST sequence type discovered in the first fatal case of Laribacter hongkongensis bacteremia clusters with the sequence types of other human isolates |
| title_full | A novel MLST sequence type discovered in the first fatal case of Laribacter hongkongensis bacteremia clusters with the sequence types of other human isolates |
| title_fullStr | A novel MLST sequence type discovered in the first fatal case of Laribacter hongkongensis bacteremia clusters with the sequence types of other human isolates |
| title_full_unstemmed | A novel MLST sequence type discovered in the first fatal case of Laribacter hongkongensis bacteremia clusters with the sequence types of other human isolates |
| title_short | A novel MLST sequence type discovered in the first fatal case of Laribacter hongkongensis bacteremia clusters with the sequence types of other human isolates |
| title_sort | novel mlst sequence type discovered in the first fatal case of laribacter hongkongensis bacteremia clusters with the sequence types of other human isolates |
| topic | bacteraemia infections |
| url | https://www.tandfonline.com/doi/10.1038/emi.2014.39 |
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