Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing
ABSTRACTWe aimed to test the common belief, that as the sperm cell is the smallest cell in an organism and, therefore, it contains a small amount of intracellular water, the intracellular ice should not strongly affect the sperm cell during the cryopreservation. To investigate the effect of intracel...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Applied Animal Research |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09712119.2023.2171045 |
| _version_ | 1797448331368071168 |
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| author | Filipp Georgijevic Savvulidi Martin Ptacek Anezka Malkova Irena Kratochvilova Daniel Simek Felipe Martinez-Pastor Ludek Stadnik |
| author_facet | Filipp Georgijevic Savvulidi Martin Ptacek Anezka Malkova Irena Kratochvilova Daniel Simek Felipe Martinez-Pastor Ludek Stadnik |
| author_sort | Filipp Georgijevic Savvulidi |
| collection | DOAJ |
| description | ABSTRACTWe aimed to test the common belief, that as the sperm cell is the smallest cell in an organism and, therefore, it contains a small amount of intracellular water, the intracellular ice should not strongly affect the sperm cell during the cryopreservation. To investigate the effect of intracellular ice, we developed a novel approach of ram spermatozoa ultra-rapid freezing with the inhibition of the growth of extracellular ice crystals during freezing. In our approach, ram sperm was supplemented with cell-impermeable synthetic ice blocker 1,4-Cyclohexanediol, leaving a sample unsupplemented as a control. Sperm in cryostraws were ultra-rapidly frozen, and then thawed. We hypothesized, that only in the case when the intracellular ice plays the role in the cryodamage, the post-freezing intactness of spermatozoa frozen in the presence of extracellular ice inhibitors should be equal (or even less) to the intactness of spermatozoa frozen without extracellular inhibitors. No statistically significant difference (p = 0.98) was observed between the post-freezing intactness of sperm ultra-rapidly frozen in the presence of 1,4-Cyclohexanediol and the intactness of sperm frozen without 1,4-Cyclohexanediol. We concluded that the intracellular ice plays the role in sperm cryodamage, at least in the model of ram sperm ultra-rapid freezing. |
| first_indexed | 2024-03-09T14:08:46Z |
| format | Article |
| id | doaj.art-6897850511c44c05a1ce3496fecbcd7d |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-03-09T14:08:46Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-6897850511c44c05a1ce3496fecbcd7d2023-11-29T18:00:51ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442023-12-0151118219210.1080/09712119.2023.2171045Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezingFilipp Georgijevic Savvulidi0Martin Ptacek1Anezka Malkova2Irena Kratochvilova3Daniel Simek4Felipe Martinez-Pastor5Ludek Stadnik6Department of Animal Science, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicDepartment of Animal Science, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicDepartment of Animal Science, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicDepartment of Functional Materials, Institute of Physics of the Czech Academy of Sciences, Prague, Czech RepublicDepartment of Functional Materials, Institute of Physics of the Czech Academy of Sciences, Prague, Czech RepublicInstituto de Desarrollo Ganadero y Sanidad Animal (INDEGSAL) and Molecular Biology (Cell Biology), Universidad de Leon, Leon, SpainDepartment of Animal Science, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicABSTRACTWe aimed to test the common belief, that as the sperm cell is the smallest cell in an organism and, therefore, it contains a small amount of intracellular water, the intracellular ice should not strongly affect the sperm cell during the cryopreservation. To investigate the effect of intracellular ice, we developed a novel approach of ram spermatozoa ultra-rapid freezing with the inhibition of the growth of extracellular ice crystals during freezing. In our approach, ram sperm was supplemented with cell-impermeable synthetic ice blocker 1,4-Cyclohexanediol, leaving a sample unsupplemented as a control. Sperm in cryostraws were ultra-rapidly frozen, and then thawed. We hypothesized, that only in the case when the intracellular ice plays the role in the cryodamage, the post-freezing intactness of spermatozoa frozen in the presence of extracellular ice inhibitors should be equal (or even less) to the intactness of spermatozoa frozen without extracellular inhibitors. No statistically significant difference (p = 0.98) was observed between the post-freezing intactness of sperm ultra-rapidly frozen in the presence of 1,4-Cyclohexanediol and the intactness of sperm frozen without 1,4-Cyclohexanediol. We concluded that the intracellular ice plays the role in sperm cryodamage, at least in the model of ram sperm ultra-rapid freezing.https://www.tandfonline.com/doi/10.1080/09712119.2023.2171045Cryodamageextracellular icesynthetic ice blockerX-ray diffraction |
| spellingShingle | Filipp Georgijevic Savvulidi Martin Ptacek Anezka Malkova Irena Kratochvilova Daniel Simek Felipe Martinez-Pastor Ludek Stadnik Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing Journal of Applied Animal Research Cryodamage extracellular ice synthetic ice blocker X-ray diffraction |
| title | Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing |
| title_full | Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing |
| title_fullStr | Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing |
| title_full_unstemmed | Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing |
| title_short | Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing |
| title_sort | inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra rapid freezing |
| topic | Cryodamage extracellular ice synthetic ice blocker X-ray diffraction |
| url | https://www.tandfonline.com/doi/10.1080/09712119.2023.2171045 |
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