Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysis
Organic forms of selenium (Se) are drawing more attention in the field of functional food. Se-enriched yeast is one of the best known approaches to supply these compounds in the form of selenomethionine. Saccharomyces cerevisiae UFMG A-905 is of particular interest as a nutritional supplement and ph...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-08-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615003424 |
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| author | Bárbara A.A. Porto Erika Mangiapane Alessandro Pessione Maria J. Neves Enrica Pessione Flaviano S. Martins |
| author_facet | Bárbara A.A. Porto Erika Mangiapane Alessandro Pessione Maria J. Neves Enrica Pessione Flaviano S. Martins |
| author_sort | Bárbara A.A. Porto |
| collection | DOAJ |
| description | Organic forms of selenium (Se) are drawing more attention in the field of functional food. Se-enriched yeast is one of the best known approaches to supply these compounds in the form of selenomethionine. Saccharomyces cerevisiae UFMG A-905 is of particular interest as a nutritional supplement and pharmaceutical since it can both fix Se and has been found to have potential for use as a probiotic. The aim of this study was to evaluate the effects of sodium selenite on this strain. A comparative proteomic approach was employed, highlighting the differences in the expression of 13 proteins in a pure YPD medium control and a sample containing 100 mg/L sodium selenite. Both proteomic and phenotypic analyses revealed that oxidative stress was caused. The analyses also revealed the ability of S. cerevisiae to set up strategies to counteract this phenomenon. In addition, the up-regulation of a cystathionine gamma-lyase confirms the ability of the strain to produce organic forms of Se that are usually more bioavailable for humans. |
| first_indexed | 2024-12-20T05:44:16Z |
| format | Article |
| id | doaj.art-689c3156cd234e649c1dd575abd1ec0c |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T05:44:16Z |
| publishDate | 2015-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-689c3156cd234e649c1dd575abd1ec0c2022-12-21T19:51:21ZengElsevierJournal of Functional Foods1756-46462015-08-0117828836Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysisBárbara A.A. Porto0Erika Mangiapane1Alessandro Pessione2Maria J. Neves3Enrica Pessione4Flaviano S. Martins5Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, BrazilLife Science and Systems Biology Department, University of Torino, Torino, ItalyLife Science and Systems Biology Department, University of Torino, Torino, ItalyNuclear Technology Development Center/National Nuclear Energy Commission (CDTN/CNEN), Belo Horizonte, MG, BrazilLife Science and Systems Biology Department, University of Torino, Torino, ItalyDepartment of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil; Corresponding author. Laboratório de Agentes Bioterapêuticos, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, C.P. 486, Pampulha – Campus UFMG, 31270-901, Belo Horizonte, MG, Brazil. Tel.: +55 31 3409 2738; fax: +55 31 3409 2730.Organic forms of selenium (Se) are drawing more attention in the field of functional food. Se-enriched yeast is one of the best known approaches to supply these compounds in the form of selenomethionine. Saccharomyces cerevisiae UFMG A-905 is of particular interest as a nutritional supplement and pharmaceutical since it can both fix Se and has been found to have potential for use as a probiotic. The aim of this study was to evaluate the effects of sodium selenite on this strain. A comparative proteomic approach was employed, highlighting the differences in the expression of 13 proteins in a pure YPD medium control and a sample containing 100 mg/L sodium selenite. Both proteomic and phenotypic analyses revealed that oxidative stress was caused. The analyses also revealed the ability of S. cerevisiae to set up strategies to counteract this phenomenon. In addition, the up-regulation of a cystathionine gamma-lyase confirms the ability of the strain to produce organic forms of Se that are usually more bioavailable for humans.http://www.sciencedirect.com/science/article/pii/S1756464615003424ProbioticSaccharomyces cerevisiaeNutraceuticalSeleniumProteomicsOxidative stress |
| spellingShingle | Bárbara A.A. Porto Erika Mangiapane Alessandro Pessione Maria J. Neves Enrica Pessione Flaviano S. Martins Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysis Journal of Functional Foods Probiotic Saccharomyces cerevisiae Nutraceutical Selenium Proteomics Oxidative stress |
| title | Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysis |
| title_full | Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysis |
| title_fullStr | Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysis |
| title_full_unstemmed | Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysis |
| title_short | Evaluation of sodium selenite effects on the potential probiotic Saccharomyces cerevisiae UFMG A-905: A physiological and proteomic analysis |
| title_sort | evaluation of sodium selenite effects on the potential probiotic saccharomyces cerevisiae ufmg a 905 a physiological and proteomic analysis |
| topic | Probiotic Saccharomyces cerevisiae Nutraceutical Selenium Proteomics Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615003424 |
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