Extraction and characterization of chitin and chitosan from Tenebrio Molitor beetles and investigation of its antibacterial effect against Pseudomonas aeruginosa
Background: Chitin and chitosan are utilized in many industries such as pharmacy, biotechnology, and medicine. The mealworm beetle, Tenebrio Molitor, is simply breaded and does not require a vast production space. Materials and Methods: In this study, we extracted chitin and chitosan using two diffe...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Advanced Biomedical Research |
| Subjects: | |
| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2023;volume=12;issue=1;spage=96;epage=96;aulast=Nafary |
| _version_ | 1797649570385100800 |
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| author | Amirhossen Nafary Seyed Amin Mousavi Nezhad Shirin Jalili |
| author_facet | Amirhossen Nafary Seyed Amin Mousavi Nezhad Shirin Jalili |
| author_sort | Amirhossen Nafary |
| collection | DOAJ |
| description | Background: Chitin and chitosan are utilized in many industries such as pharmacy, biotechnology, and medicine. The mealworm beetle, Tenebrio Molitor, is simply breaded and does not require a vast production space.
Materials and Methods: In this study, we extracted chitin and chitosan using two different methods from Tenebrio Molitor adult beetles. Then we studied their physical and chemical properties along with their antibacterial effect.
Results: Using two new methods we extracted 13, 3%, and 17.7% chitin from the dry mealworm beetle which was higher than in previous studies. The chitosan yield of the extracted chitin was 78.26% and 76.43%, respectively. The observed FTIR peaks for chitin and chitosan in this study were in accordance with the characteristic peaks. The degree of acetylation of chitin was 95.09% and 92.55% and the degree of deacetylation was 75.84%, and 72.6% from the first and second methods, respectively. The extracted chitosan also showed an antibacterial effect against Pseudomonas aeruginosa.
Conclusions: Our study demonstrated that chitin and chitosan extracted from adult mealworm beetles could be considered as a replacement for commercial chitosan and needs further studies. |
| first_indexed | 2024-03-11T15:48:02Z |
| format | Article |
| id | doaj.art-68a2d109406e467fb359761fa2416be3 |
| institution | Directory Open Access Journal |
| issn | 2277-9175 |
| language | English |
| last_indexed | 2024-03-11T15:48:02Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-68a2d109406e467fb359761fa2416be32023-10-26T05:43:16ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752023-01-01121969610.4103/abr.abr_205_22Extraction and characterization of chitin and chitosan from Tenebrio Molitor beetles and investigation of its antibacterial effect against Pseudomonas aeruginosaAmirhossen NafarySeyed Amin Mousavi NezhadShirin JaliliBackground: Chitin and chitosan are utilized in many industries such as pharmacy, biotechnology, and medicine. The mealworm beetle, Tenebrio Molitor, is simply breaded and does not require a vast production space. Materials and Methods: In this study, we extracted chitin and chitosan using two different methods from Tenebrio Molitor adult beetles. Then we studied their physical and chemical properties along with their antibacterial effect. Results: Using two new methods we extracted 13, 3%, and 17.7% chitin from the dry mealworm beetle which was higher than in previous studies. The chitosan yield of the extracted chitin was 78.26% and 76.43%, respectively. The observed FTIR peaks for chitin and chitosan in this study were in accordance with the characteristic peaks. The degree of acetylation of chitin was 95.09% and 92.55% and the degree of deacetylation was 75.84%, and 72.6% from the first and second methods, respectively. The extracted chitosan also showed an antibacterial effect against Pseudomonas aeruginosa. Conclusions: Our study demonstrated that chitin and chitosan extracted from adult mealworm beetles could be considered as a replacement for commercial chitosan and needs further studies.http://www.advbiores.net/article.asp?issn=2277-9175;year=2023;volume=12;issue=1;spage=96;epage=96;aulast=Nafarychitinchitosanpseudomonas aeruginosa |
| spellingShingle | Amirhossen Nafary Seyed Amin Mousavi Nezhad Shirin Jalili Extraction and characterization of chitin and chitosan from Tenebrio Molitor beetles and investigation of its antibacterial effect against Pseudomonas aeruginosa Advanced Biomedical Research chitin chitosan pseudomonas aeruginosa |
| title | Extraction and characterization of chitin and chitosan from Tenebrio Molitor beetles and investigation of its antibacterial effect against Pseudomonas aeruginosa |
| title_full | Extraction and characterization of chitin and chitosan from Tenebrio Molitor beetles and investigation of its antibacterial effect against Pseudomonas aeruginosa |
| title_fullStr | Extraction and characterization of chitin and chitosan from Tenebrio Molitor beetles and investigation of its antibacterial effect against Pseudomonas aeruginosa |
| title_full_unstemmed | Extraction and characterization of chitin and chitosan from Tenebrio Molitor beetles and investigation of its antibacterial effect against Pseudomonas aeruginosa |
| title_short | Extraction and characterization of chitin and chitosan from Tenebrio Molitor beetles and investigation of its antibacterial effect against Pseudomonas aeruginosa |
| title_sort | extraction and characterization of chitin and chitosan from tenebrio molitor beetles and investigation of its antibacterial effect against pseudomonas aeruginosa |
| topic | chitin chitosan pseudomonas aeruginosa |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2023;volume=12;issue=1;spage=96;epage=96;aulast=Nafary |
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