Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane
<p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using ant...
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| Format: | Article |
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BMC
2007-05-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/7/38 |
| _version_ | 1818541990959120384 |
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| author | Liu Kaiyang Jia Tianjun Zhong Youmin Liu Guangchao Luo Jianhua Chen Ding Zhong Guangming |
| author_facet | Liu Kaiyang Jia Tianjun Zhong Youmin Liu Guangchao Luo Jianhua Chen Ding Zhong Guangming |
| author_sort | Liu Kaiyang |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (<it>Cpn0146-147 </it>and <it>Cpn0284-285</it>) in the <it>C. pneumoniae </it>genome.</p> <p>Results</p> <p>Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with <it>C. pneumoniae </it>but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the <it>C. pneumoniae </it>inclusion membrane proteins did not result in inhibition of the subsequent <it>C. pneumoniae </it>infection.</p> <p>Conclusion</p> <p>The hypothetical proteins Cpn0146 & 0147 were localized in the <it>C. pneumoniae </it>inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.</p> |
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| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T22:16:20Z |
| publishDate | 2007-05-01 |
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| series | BMC Microbiology |
| spelling | doaj.art-68a34e64727848539317b920a004ed272022-12-22T00:48:36ZengBMCBMC Microbiology1471-21802007-05-01713810.1186/1471-2180-7-38Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membraneLiu KaiyangJia TianjunZhong YouminLiu GuangchaoLuo JianhuaChen DingZhong Guangming<p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (<it>Cpn0146-147 </it>and <it>Cpn0284-285</it>) in the <it>C. pneumoniae </it>genome.</p> <p>Results</p> <p>Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with <it>C. pneumoniae </it>but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the <it>C. pneumoniae </it>inclusion membrane proteins did not result in inhibition of the subsequent <it>C. pneumoniae </it>infection.</p> <p>Conclusion</p> <p>The hypothetical proteins Cpn0146 & 0147 were localized in the <it>C. pneumoniae </it>inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.</p>http://www.biomedcentral.com/1471-2180/7/38 |
| spellingShingle | Liu Kaiyang Jia Tianjun Zhong Youmin Liu Guangchao Luo Jianhua Chen Ding Zhong Guangming Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane BMC Microbiology |
| title | Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
| title_full | Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
| title_fullStr | Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
| title_full_unstemmed | Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
| title_short | Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
| title_sort | characterization of hypothetical proteins cpn0146 0147 0284 0285 that are predicted to be in the it chlamydia pneumoniae it inclusion membrane |
| url | http://www.biomedcentral.com/1471-2180/7/38 |
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