Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, Nigeria
The dominant bacteriological and archaeal phyla of compounded soils sourced from a commercial farm estate located in Amukpe town and a nearby control in Adavware community both in Delta State, were evaluated with the aid of Next Generation Sequencing (NGS) protocols. The residual herbicide and pes...
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2019-02-01
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| Series: | Journal of Applied Sciences and Environmental Management |
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| Online Access: | https://www.ajol.info/index.php/jasem/article/view/183412 |
| _version_ | 1797227969046904832 |
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| author | A.G. Jaboro S.E. Omonigho |
| author_facet | A.G. Jaboro S.E. Omonigho |
| author_sort | A.G. Jaboro |
| collection | DOAJ |
| description |
The dominant bacteriological and archaeal phyla of compounded soils sourced from a commercial farm estate located in Amukpe town and a nearby control in Adavware community both in Delta State, were evaluated with the aid of Next Generation Sequencing (NGS) protocols. The residual herbicide and pesticide composition of the bulked soils were also determined using gas chromatography (GC) and electron capture detector (ECD). Total concentrations of the extracted DNA were 6.83 and 6.65 ng/μl for the control and experimental soils. Nine (9) bacterial phyla; Proteobacteria, Actinobacteria, Chloroflexi, Firmicutes, Verrucomicrobia, Planctomycetes, Bacteroidetes Acidobacteria, and Elusimicrobia were observed in the control soil. Thirteen (13) bacterial phyla; Elusimicrobia, Fibrobacteres Lentisphaerae, Armatimonadetes, Cyanobacteria/Chloroplast, Bacteroidetes, Actinobacteria, Proteobacteria, Chloroflexi, Firmicutes, Acidobacteria, Planctomycetes and Verrucomicrobia were detected in the experimental soil. Two (2) archaeal phyla; Euryarchaeota, and Diapherotrites were detected both the experimental and control soil, whilst an additional archaeal phylum; Woesearchaeota was present in only the experimental soil. The total organochloride phosphate component of the experimental soil was 1.4μg/Kg and 0.4μg/Kg for the control soil respectively.
Keywords: Amukpe, Next Generation Sequencing, Adavware, bacterial and archaeal phyla
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| first_indexed | 2024-04-24T14:49:14Z |
| format | Article |
| id | doaj.art-68a4fa60e4a1495d95df751fd96e5a64 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:49:14Z |
| publishDate | 2019-02-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-68a4fa60e4a1495d95df751fd96e5a642024-04-02T19:51:12ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992019-02-0123110.4314/jasem.v23i1.2Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, NigeriaA.G. JaboroS.E. Omonigho The dominant bacteriological and archaeal phyla of compounded soils sourced from a commercial farm estate located in Amukpe town and a nearby control in Adavware community both in Delta State, were evaluated with the aid of Next Generation Sequencing (NGS) protocols. The residual herbicide and pesticide composition of the bulked soils were also determined using gas chromatography (GC) and electron capture detector (ECD). Total concentrations of the extracted DNA were 6.83 and 6.65 ng/μl for the control and experimental soils. Nine (9) bacterial phyla; Proteobacteria, Actinobacteria, Chloroflexi, Firmicutes, Verrucomicrobia, Planctomycetes, Bacteroidetes Acidobacteria, and Elusimicrobia were observed in the control soil. Thirteen (13) bacterial phyla; Elusimicrobia, Fibrobacteres Lentisphaerae, Armatimonadetes, Cyanobacteria/Chloroplast, Bacteroidetes, Actinobacteria, Proteobacteria, Chloroflexi, Firmicutes, Acidobacteria, Planctomycetes and Verrucomicrobia were detected in the experimental soil. Two (2) archaeal phyla; Euryarchaeota, and Diapherotrites were detected both the experimental and control soil, whilst an additional archaeal phylum; Woesearchaeota was present in only the experimental soil. The total organochloride phosphate component of the experimental soil was 1.4μg/Kg and 0.4μg/Kg for the control soil respectively. Keywords: Amukpe, Next Generation Sequencing, Adavware, bacterial and archaeal phyla https://www.ajol.info/index.php/jasem/article/view/183412AmukpeNext Generation SequencingAdavwarebacterial and archaeal phyla |
| spellingShingle | A.G. Jaboro S.E. Omonigho Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, Nigeria Journal of Applied Sciences and Environmental Management Amukpe Next Generation Sequencing Adavware bacterial and archaeal phyla |
| title | Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, Nigeria |
| title_full | Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, Nigeria |
| title_fullStr | Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, Nigeria |
| title_full_unstemmed | Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, Nigeria |
| title_short | Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, Nigeria |
| title_sort | dominant bacterial and archaeal phyla associated with top soils sourced from commercial farm holding in delta state nigeria |
| topic | Amukpe Next Generation Sequencing Adavware bacterial and archaeal phyla |
| url | https://www.ajol.info/index.php/jasem/article/view/183412 |
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