Quantitative Assessment of Histone H2B Monoubiquitination in Yeast Using Immunoblotting

Quantitative Assessment of Histone H2B Monoubiquitination in Yeast Using Immunoblotting

Studies in <i>Saccharomyces cerevisiae</i> and <i>Schizosaccharomyces pombe</i> have enhanced our understanding of the regulation and functions of histone H2B monoubiquitination (H2Bub1), a key epigenetic marker with important roles in transcription and other processes. The d...

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Bibliographic Details
Main Authors: Andrew M. Leng, Kaitlin S. Radmall, Prakash K. Shukla, Mahesh B. Chandrasekharan
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Methods and Protocols
Subjects:
histone H2B monoubiquitination
histones
histone modification
epigenetics
Western blot
immunoblot
Online Access:https://www.mdpi.com/2409-9279/5/5/74

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