Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network
Because chromatin determines whether information encoded in DNA is accessible to transcription factors, dynamic chromatin states in development may constrain how gene regulatory networks impart embryonic pattern. To determine the interplay between chromatin states and regulatory network function, we...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-04-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/53916 |
| _version_ | 1811253068365299712 |
|---|---|
| author | Isabella V Soluri Lauren M Zumerling Omar A Payan Parra Eleanor G Clark Shelby A Blythe |
| author_facet | Isabella V Soluri Lauren M Zumerling Omar A Payan Parra Eleanor G Clark Shelby A Blythe |
| author_sort | Isabella V Soluri |
| collection | DOAJ |
| description | Because chromatin determines whether information encoded in DNA is accessible to transcription factors, dynamic chromatin states in development may constrain how gene regulatory networks impart embryonic pattern. To determine the interplay between chromatin states and regulatory network function, we performed ATAC-seq on Drosophila embryos during the establishment of the segmentation network, comparing wild-type and mutant embryos in which all graded maternal patterning inputs are eliminated. While during the period between zygotic genome activation and gastrulation many regions maintain stable accessibility, cis-regulatory modules (CRMs) within the network undergo extensive patterning-dependent changes in accessibility. A component of the network, Odd-paired (opa), is necessary for pioneering accessibility of late segmentation network CRMs. opa-driven changes in accessibility are accompanied by equivalent changes in gene expression. Interfering with the timing of opa activity impacts the proper patterning of expression. These results indicate that dynamic systems for chromatin regulation directly impact the reading of embryonic patterning information. |
| first_indexed | 2024-04-12T16:44:16Z |
| format | Article |
| id | doaj.art-68ac1b33544f4e35893a6eb094be0309 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:44:16Z |
| publishDate | 2020-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-68ac1b33544f4e35893a6eb094be03092022-12-22T03:24:38ZengeLife Sciences Publications LtdeLife2050-084X2020-04-01910.7554/eLife.53916Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation networkIsabella V Soluri0Lauren M Zumerling1Omar A Payan Parra2https://orcid.org/0000-0002-0005-8355Eleanor G Clark3Shelby A Blythe4https://orcid.org/0000-0003-4986-2579Department of Molecular Biosciences, Northwestern University, Evanston, United StatesDepartment of Molecular Biosciences, Northwestern University, Evanston, United StatesProgram in Interdisciplinary Biological Sciences, Northwestern University, Evanston, United States; Department of Neurobiology, Northwestern University, Evanston, United StatesProgram in Interdisciplinary Biological Sciences, Northwestern University, Evanston, United StatesDepartment of Molecular Biosciences, Northwestern University, Evanston, United StatesBecause chromatin determines whether information encoded in DNA is accessible to transcription factors, dynamic chromatin states in development may constrain how gene regulatory networks impart embryonic pattern. To determine the interplay between chromatin states and regulatory network function, we performed ATAC-seq on Drosophila embryos during the establishment of the segmentation network, comparing wild-type and mutant embryos in which all graded maternal patterning inputs are eliminated. While during the period between zygotic genome activation and gastrulation many regions maintain stable accessibility, cis-regulatory modules (CRMs) within the network undergo extensive patterning-dependent changes in accessibility. A component of the network, Odd-paired (opa), is necessary for pioneering accessibility of late segmentation network CRMs. opa-driven changes in accessibility are accompanied by equivalent changes in gene expression. Interfering with the timing of opa activity impacts the proper patterning of expression. These results indicate that dynamic systems for chromatin regulation directly impact the reading of embryonic patterning information.https://elifesciences.org/articles/53916patterningtranscription factorschromatin |
| spellingShingle | Isabella V Soluri Lauren M Zumerling Omar A Payan Parra Eleanor G Clark Shelby A Blythe Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network eLife patterning transcription factors chromatin |
| title | Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network |
| title_full | Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network |
| title_fullStr | Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network |
| title_full_unstemmed | Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network |
| title_short | Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network |
| title_sort | zygotic pioneer factor activity of odd paired zic is necessary for late function of the drosophila segmentation network |
| topic | patterning transcription factors chromatin |
| url | https://elifesciences.org/articles/53916 |
| work_keys_str_mv | AT isabellavsoluri zygoticpioneerfactoractivityofoddpairedzicisnecessaryforlatefunctionofthedrosophilasegmentationnetwork AT laurenmzumerling zygoticpioneerfactoractivityofoddpairedzicisnecessaryforlatefunctionofthedrosophilasegmentationnetwork AT omarapayanparra zygoticpioneerfactoractivityofoddpairedzicisnecessaryforlatefunctionofthedrosophilasegmentationnetwork AT eleanorgclark zygoticpioneerfactoractivityofoddpairedzicisnecessaryforlatefunctionofthedrosophilasegmentationnetwork AT shelbyablythe zygoticpioneerfactoractivityofoddpairedzicisnecessaryforlatefunctionofthedrosophilasegmentationnetwork |