<i>Haloferax profundi</i> sp. nov. and <i>Haloferax marisrubri</i> sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea
Two extremely halophilic archaeal strains, designated SB29<sup>T</sup> and SB3<sup>T</sup>, were isolated from the brine-seawater interface of Discovery Deep in the Red Sea. Cells of both strains were pleomorphic (irregular polyhedrals, ovals, and rods) and stained Gram-negat...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/10/1475 |
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| author | Guishan Zhang Xiaoyan Dong Yingjiao Sun André Antunes Tyas Hikmawan Mohamed Fauzi Haroon Junru Wang Ulrich Stingl |
| author_facet | Guishan Zhang Xiaoyan Dong Yingjiao Sun André Antunes Tyas Hikmawan Mohamed Fauzi Haroon Junru Wang Ulrich Stingl |
| author_sort | Guishan Zhang |
| collection | DOAJ |
| description | Two extremely halophilic archaeal strains, designated SB29<sup>T</sup> and SB3<sup>T</sup>, were isolated from the brine-seawater interface of Discovery Deep in the Red Sea. Cells of both strains were pleomorphic (irregular polyhedrals, ovals, and rods) and stained Gram-negative; colonies were pigmented pink. The sequence similarity of the 16S rRNA gene of strain SB29<sup>T</sup> with that of its most closely related validly described species (<i>Hfx. sulfurifontis</i> DSM 16227<sup>T</sup>) and that of strain SB3<sup>T</sup> with its closest validly described relative (<i>Hfx. denitrificans</i> ATCC 35960<sup>T</sup>) was 98.1% and 98.6%, respectively. The incomplete draft genomes of SB29<sup>T</sup> and SB3<sup>T</sup> are 3,871,125 bp and 3,904,985 bp in size, respectively, and their DNA G + C contents are 60.75% and 65.64%, respectively. The highest ANI values between the genomes of SB29<sup>T</sup> and SB3<sup>T</sup> and the most closely related genomes in GenBank were determined as 82.6% (<i>Hfx. sulfurifontis</i> ATCC BAA-897<sup>T</sup>, GenBank accession no. GCA_000337835.1) and 92.6% (<i>Haloferax denitrificans</i> ATCC 35960<sup>T</sup>, GenBank accession no. GCA_000337795.1), respectively. These data indicate that the two new isolates cannot be classified into any recognized species of the genus <i>Haloferax</i>, and, therefore, two novel species of the genus <i>Haloferax</i> are proposed: <i>Haloferax profundi</i> sp. nov. (type strain SB29<sup>T</sup> = JCM 19567<sup>T</sup> = CGMCC 1.14960<sup>T</sup>) and <i>Haloferax marisrubri</i> sp. nov. (type strain SB3<sup>T</sup> = JCM 19566<sup>T</sup> = CGMCC 1.14958<sup>T</sup>). |
| first_indexed | 2024-03-10T16:02:42Z |
| format | Article |
| id | doaj.art-68afbe82af6c4e41958558dfff3f1c93 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T16:02:42Z |
| publishDate | 2020-09-01 |
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| spelling | doaj.art-68afbe82af6c4e41958558dfff3f1c932023-11-20T15:08:24ZengMDPI AGMicroorganisms2076-26072020-09-01810147510.3390/microorganisms8101475<i>Haloferax profundi</i> sp. nov. and <i>Haloferax marisrubri</i> sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red SeaGuishan Zhang0Xiaoyan Dong1Yingjiao Sun2André Antunes3Tyas Hikmawan4Mohamed Fauzi Haroon5Junru Wang6Ulrich Stingl7Red Sea Research Center, King Abdullah University of Science and Technology, Thuwal Jeddah 23955-6900, Saudi ArabiaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaRed Sea Research Center, King Abdullah University of Science and Technology, Thuwal Jeddah 23955-6900, Saudi ArabiaRed Sea Research Center, King Abdullah University of Science and Technology, Thuwal Jeddah 23955-6900, Saudi ArabiaRed Sea Research Center, King Abdullah University of Science and Technology, Thuwal Jeddah 23955-6900, Saudi ArabiaKey Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaRed Sea Research Center, King Abdullah University of Science and Technology, Thuwal Jeddah 23955-6900, Saudi ArabiaTwo extremely halophilic archaeal strains, designated SB29<sup>T</sup> and SB3<sup>T</sup>, were isolated from the brine-seawater interface of Discovery Deep in the Red Sea. Cells of both strains were pleomorphic (irregular polyhedrals, ovals, and rods) and stained Gram-negative; colonies were pigmented pink. The sequence similarity of the 16S rRNA gene of strain SB29<sup>T</sup> with that of its most closely related validly described species (<i>Hfx. sulfurifontis</i> DSM 16227<sup>T</sup>) and that of strain SB3<sup>T</sup> with its closest validly described relative (<i>Hfx. denitrificans</i> ATCC 35960<sup>T</sup>) was 98.1% and 98.6%, respectively. The incomplete draft genomes of SB29<sup>T</sup> and SB3<sup>T</sup> are 3,871,125 bp and 3,904,985 bp in size, respectively, and their DNA G + C contents are 60.75% and 65.64%, respectively. The highest ANI values between the genomes of SB29<sup>T</sup> and SB3<sup>T</sup> and the most closely related genomes in GenBank were determined as 82.6% (<i>Hfx. sulfurifontis</i> ATCC BAA-897<sup>T</sup>, GenBank accession no. GCA_000337835.1) and 92.6% (<i>Haloferax denitrificans</i> ATCC 35960<sup>T</sup>, GenBank accession no. GCA_000337795.1), respectively. These data indicate that the two new isolates cannot be classified into any recognized species of the genus <i>Haloferax</i>, and, therefore, two novel species of the genus <i>Haloferax</i> are proposed: <i>Haloferax profundi</i> sp. nov. (type strain SB29<sup>T</sup> = JCM 19567<sup>T</sup> = CGMCC 1.14960<sup>T</sup>) and <i>Haloferax marisrubri</i> sp. nov. (type strain SB3<sup>T</sup> = JCM 19566<sup>T</sup> = CGMCC 1.14958<sup>T</sup>).https://www.mdpi.com/2076-2607/8/10/1475<i>Haloferax profundi</i><i>Haloferax marisrubri</i>halophilic archaeonbrine pool |
| spellingShingle | Guishan Zhang Xiaoyan Dong Yingjiao Sun André Antunes Tyas Hikmawan Mohamed Fauzi Haroon Junru Wang Ulrich Stingl <i>Haloferax profundi</i> sp. nov. and <i>Haloferax marisrubri</i> sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea Microorganisms <i>Haloferax profundi</i> <i>Haloferax marisrubri</i> halophilic archaeon brine pool |
| title | <i>Haloferax profundi</i> sp. nov. and <i>Haloferax marisrubri</i> sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea |
| title_full | <i>Haloferax profundi</i> sp. nov. and <i>Haloferax marisrubri</i> sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea |
| title_fullStr | <i>Haloferax profundi</i> sp. nov. and <i>Haloferax marisrubri</i> sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea |
| title_full_unstemmed | <i>Haloferax profundi</i> sp. nov. and <i>Haloferax marisrubri</i> sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea |
| title_short | <i>Haloferax profundi</i> sp. nov. and <i>Haloferax marisrubri</i> sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea |
| title_sort | i haloferax profundi i sp nov and i haloferax marisrubri i sp nov isolated from the discovery deep brine seawater interface in the red sea |
| topic | <i>Haloferax profundi</i> <i>Haloferax marisrubri</i> halophilic archaeon brine pool |
| url | https://www.mdpi.com/2076-2607/8/10/1475 |
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