<i>CaDHN3</i>, a Pepper (<i>Capsicum annuum</i> L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation
Dehydrins (DHNs) play an important role in abiotic stress tolerance in a large number of plants, but very little is known about the function of DHNs in pepper plants. Here, we isolated a Y<sub>1</sub>SK<sub>2</sub>-type DHN gene “<i>CaDHN3</i>” from pepper. To aut...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/6/3205 |
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| author | Yuan-Cheng Meng Hua-Feng Zhang Xiao-Xiao Pan Nan Chen Hui-Fang Hu Saeed ul Haq Abid Khan Ru-Gang Chen |
| author_facet | Yuan-Cheng Meng Hua-Feng Zhang Xiao-Xiao Pan Nan Chen Hui-Fang Hu Saeed ul Haq Abid Khan Ru-Gang Chen |
| author_sort | Yuan-Cheng Meng |
| collection | DOAJ |
| description | Dehydrins (DHNs) play an important role in abiotic stress tolerance in a large number of plants, but very little is known about the function of DHNs in pepper plants. Here, we isolated a Y<sub>1</sub>SK<sub>2</sub>-type DHN gene “<i>CaDHN3</i>” from pepper. To authenticate the function of <i>CaDHN3</i> in salt and drought stresses, it was overexpressed in Arabidopsis and silenced in pepper through virus-induced gene silencing (VIGS). Sub-cellular localization showed that <i>CaDHN3</i> was located in the nucleus and cell membrane. It was found that <i>CaDHN3</i>-overexpressed (OE) in Arabidopsis plants showed salt and drought tolerance phenotypic characteristics, i.e., increased the initial rooting length and germination rate, enhanced chlorophyll content, lowered the relative electrolyte leakage (REL) and malondialdehyde (MDA) content than the wild-type (WT) plants. Moreover, a substantial increase in the activities of antioxidant enzymes; including the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and lower hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) contents and higher O<sub>2</sub><sup>•−</sup> contents in the transgenic Arabidopsis plants. Silencing of <i>CaDHN3</i> in pepper decreased the salt- and drought-stress tolerance, through a higher REL and MDA content, and there was more accumulation of reactive oxygen species (ROS) in the <i>CaDHN3</i>-silenced pepper plants than the control plants. Based on the yeast two-hybrid (Y2H) screening and Bimolecular Fluorescence Complementation (BiFC) results, we found that CaDHN3 interacts with CaHIRD11 protein in the plasma membrane. Correspondingly, the expressions of four osmotic-related genes were significantly up-regulated in the <i>CaDHN3</i>-overexpressed lines. In brief, our results manifested that <i>CaDHN3</i> may play an important role in regulating the relative osmotic stress responses in plants through the ROS signaling pathway. The results of this study will provide a basis for further analyses of the function of DHN genes in pepper. |
| first_indexed | 2024-03-10T13:02:04Z |
| format | Article |
| id | doaj.art-68b26145ad7642efb17d90b1076d54a5 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:02:04Z |
| publishDate | 2021-03-01 |
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| spelling | doaj.art-68b26145ad7642efb17d90b1076d54a52023-11-21T11:26:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01226320510.3390/ijms22063205<i>CaDHN3</i>, a Pepper (<i>Capsicum annuum</i> L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS AccumulationYuan-Cheng Meng0Hua-Feng Zhang1Xiao-Xiao Pan2Nan Chen3Hui-Fang Hu4Saeed ul Haq5Abid Khan6Ru-Gang Chen7College of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaDehydrins (DHNs) play an important role in abiotic stress tolerance in a large number of plants, but very little is known about the function of DHNs in pepper plants. Here, we isolated a Y<sub>1</sub>SK<sub>2</sub>-type DHN gene “<i>CaDHN3</i>” from pepper. To authenticate the function of <i>CaDHN3</i> in salt and drought stresses, it was overexpressed in Arabidopsis and silenced in pepper through virus-induced gene silencing (VIGS). Sub-cellular localization showed that <i>CaDHN3</i> was located in the nucleus and cell membrane. It was found that <i>CaDHN3</i>-overexpressed (OE) in Arabidopsis plants showed salt and drought tolerance phenotypic characteristics, i.e., increased the initial rooting length and germination rate, enhanced chlorophyll content, lowered the relative electrolyte leakage (REL) and malondialdehyde (MDA) content than the wild-type (WT) plants. Moreover, a substantial increase in the activities of antioxidant enzymes; including the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and lower hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) contents and higher O<sub>2</sub><sup>•−</sup> contents in the transgenic Arabidopsis plants. Silencing of <i>CaDHN3</i> in pepper decreased the salt- and drought-stress tolerance, through a higher REL and MDA content, and there was more accumulation of reactive oxygen species (ROS) in the <i>CaDHN3</i>-silenced pepper plants than the control plants. Based on the yeast two-hybrid (Y2H) screening and Bimolecular Fluorescence Complementation (BiFC) results, we found that CaDHN3 interacts with CaHIRD11 protein in the plasma membrane. Correspondingly, the expressions of four osmotic-related genes were significantly up-regulated in the <i>CaDHN3</i>-overexpressed lines. In brief, our results manifested that <i>CaDHN3</i> may play an important role in regulating the relative osmotic stress responses in plants through the ROS signaling pathway. The results of this study will provide a basis for further analyses of the function of DHN genes in pepper.https://www.mdpi.com/1422-0067/22/6/3205dehydrins<i>CaDHN3</i>overexpressionabiotic stresses |
| spellingShingle | Yuan-Cheng Meng Hua-Feng Zhang Xiao-Xiao Pan Nan Chen Hui-Fang Hu Saeed ul Haq Abid Khan Ru-Gang Chen <i>CaDHN3</i>, a Pepper (<i>Capsicum annuum</i> L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation International Journal of Molecular Sciences dehydrins <i>CaDHN3</i> overexpression abiotic stresses |
| title | <i>CaDHN3</i>, a Pepper (<i>Capsicum annuum</i> L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation |
| title_full | <i>CaDHN3</i>, a Pepper (<i>Capsicum annuum</i> L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation |
| title_fullStr | <i>CaDHN3</i>, a Pepper (<i>Capsicum annuum</i> L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation |
| title_full_unstemmed | <i>CaDHN3</i>, a Pepper (<i>Capsicum annuum</i> L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation |
| title_short | <i>CaDHN3</i>, a Pepper (<i>Capsicum annuum</i> L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation |
| title_sort | i cadhn3 i a pepper i capsicum annuum i l dehydrin gene enhances the tolerance against salt and drought stresses by reducing ros accumulation |
| topic | dehydrins <i>CaDHN3</i> overexpression abiotic stresses |
| url | https://www.mdpi.com/1422-0067/22/6/3205 |
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