Peri-operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgery
Abstract Bariatric surgery is the most effective treatment for weight loss. Vertical sleeve gastrectomy (VSG) involves the resection of ~ 80% of the stomach and was conceived to purely restrict oral intake. However, evidence suggests more complex mechanisms, particularly postoperative changes in gut...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-77285-7 |
| _version_ | 1818429449790554112 |
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| author | Harika Nalluri Scott Kizy Kristin Ewing Girish Luthra Daniel B. Leslie David A. Bernlohr Michael J. Sadowsky Sayeed Ikramuddin Alexander Khoruts Christopher Staley Cyrus Jahansouz |
| author_facet | Harika Nalluri Scott Kizy Kristin Ewing Girish Luthra Daniel B. Leslie David A. Bernlohr Michael J. Sadowsky Sayeed Ikramuddin Alexander Khoruts Christopher Staley Cyrus Jahansouz |
| author_sort | Harika Nalluri |
| collection | DOAJ |
| description | Abstract Bariatric surgery is the most effective treatment for weight loss. Vertical sleeve gastrectomy (VSG) involves the resection of ~ 80% of the stomach and was conceived to purely restrict oral intake. However, evidence suggests more complex mechanisms, particularly postoperative changes in gut microbiota, in facilitating weight loss and resolving associated comorbidities. VSG in humans is a complex procedure and includes peri-operative antibiotics and caloric restriction in addition to the altered anatomy. The impact of each of these factors on the intestinal microbiota have not been evaluated. The aim of this study was to determine the relative contributions of each of these factors on intestinal microbiota composition following VSG prior to substantial weight loss. Thirty-two obese patients underwent one of three treatments: (1) VSG plus routine intravenous peri-operative antibiotics (n = 12), (2) VSG with intravenous vancomycin chosen for its low intestinal penetrance (n = 12), and (3) caloric restriction (n = 8). Fecal samples were evaluated for bacterial composition prior to and 7 days following each intervention. Only patients undergoing VSG with routine peri-operative antibiotics showed a significant shift in community composition. Our data support the single dose of routine peri-operative antibiotics as the most influential factor of intestinal microbial composition acutely following VSG. |
| first_indexed | 2024-12-14T15:17:42Z |
| format | Article |
| id | doaj.art-68b45cb152d840b2a39560c51d673df1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:17:42Z |
| publishDate | 2020-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-68b45cb152d840b2a39560c51d673df12022-12-21T22:56:15ZengNature PortfolioScientific Reports2045-23222020-11-0110111010.1038/s41598-020-77285-7Peri-operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgeryHarika Nalluri0Scott Kizy1Kristin Ewing2Girish Luthra3Daniel B. Leslie4David A. Bernlohr5Michael J. Sadowsky6Sayeed Ikramuddin7Alexander Khoruts8Christopher Staley9Cyrus Jahansouz10Department of Surgery, University of MinnesotaDepartment of Surgery, University of MinnesotaCentraCare Bariatric CenterPark Nicollet Bariatric Surgery CenterDepartment of Surgery, University of MinnesotaDepartment of Biochemistry, Molecular Biology and Biophysics, University of MinnesotaBioTechnology Institute, University of MinnesotaDepartment of Surgery, University of MinnesotaBioTechnology Institute, University of MinnesotaDepartment of Surgery, University of MinnesotaDepartment of Surgery, University of MinnesotaAbstract Bariatric surgery is the most effective treatment for weight loss. Vertical sleeve gastrectomy (VSG) involves the resection of ~ 80% of the stomach and was conceived to purely restrict oral intake. However, evidence suggests more complex mechanisms, particularly postoperative changes in gut microbiota, in facilitating weight loss and resolving associated comorbidities. VSG in humans is a complex procedure and includes peri-operative antibiotics and caloric restriction in addition to the altered anatomy. The impact of each of these factors on the intestinal microbiota have not been evaluated. The aim of this study was to determine the relative contributions of each of these factors on intestinal microbiota composition following VSG prior to substantial weight loss. Thirty-two obese patients underwent one of three treatments: (1) VSG plus routine intravenous peri-operative antibiotics (n = 12), (2) VSG with intravenous vancomycin chosen for its low intestinal penetrance (n = 12), and (3) caloric restriction (n = 8). Fecal samples were evaluated for bacterial composition prior to and 7 days following each intervention. Only patients undergoing VSG with routine peri-operative antibiotics showed a significant shift in community composition. Our data support the single dose of routine peri-operative antibiotics as the most influential factor of intestinal microbial composition acutely following VSG.https://doi.org/10.1038/s41598-020-77285-7 |
| spellingShingle | Harika Nalluri Scott Kizy Kristin Ewing Girish Luthra Daniel B. Leslie David A. Bernlohr Michael J. Sadowsky Sayeed Ikramuddin Alexander Khoruts Christopher Staley Cyrus Jahansouz Peri-operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgery Scientific Reports |
| title | Peri-operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgery |
| title_full | Peri-operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgery |
| title_fullStr | Peri-operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgery |
| title_full_unstemmed | Peri-operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgery |
| title_short | Peri-operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgery |
| title_sort | peri operative antibiotics acutely and significantly impact intestinal microbiota following bariatric surgery |
| url | https://doi.org/10.1038/s41598-020-77285-7 |
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