Enhancing the efficacy of Hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles: an in vivo study
Objective: Hypericum perforatum is a herbal medicine used in traditional medicine for the treatment of depression due to its antidepressant and anti-inflammatory activities. Therefore, we evaluated the therapeutic efficacy of H. perforatum extract (HPE) in combination with gold nanoparticles (HPE-GN...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-05-01
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| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | https://ajp.mums.ac.ir/article_19574_d8ef1a0c3ada92f1ac801aedf2a989c2.pdf |
| _version_ | 1818272594386747392 |
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| author | Mahmoud Mahmoudi Maryam Rastin Mohammad Kazemi Arababadi Akbar Anaeigoudari Reza Nosratabadi |
| author_facet | Mahmoud Mahmoudi Maryam Rastin Mohammad Kazemi Arababadi Akbar Anaeigoudari Reza Nosratabadi |
| author_sort | Mahmoud Mahmoudi |
| collection | DOAJ |
| description | Objective: Hypericum perforatum is a herbal medicine used in traditional medicine for the treatment of depression due to its antidepressant and anti-inflammatory activities. Therefore, we evaluated the therapeutic efficacy of H. perforatum extract (HPE) in combination with gold nanoparticles (HPE-GNP) against experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. Materials and Methods: EAE was induced in C57BL/6 mice with subcutaneous injection of MOG35-55 emulsified in complete Freund's adjuvant, and intraperitoneal pertussis toxin. Mice were treated with drugs in free (HPE) and nano-form (HPE-GNP) preparations. Splenocytes were isolated from all mice and the level of inflammatory and anti-inflammatory cytokines were evaluated by ELISA. The expression of T cells' transcription factors was also assessed using Real-Time PCR.Results: Clinical score was reduced after HPE-GNP treatment. This change was associated with a decrease in the incidence and infiltration of inflammatory cells into the central nervous system. Additionally, treatment with HPE-GNP decreased the level of pro-inflammatory cytokines (IFN-γ, IL-17A and IL-6) and increased anti-inflammatory cytokines (TGF-β, IL-10 and IL-4). The real-time analysis revealed a decrease in the level of T-bet and ROR-γt but an increase in FoxP3 and GATA3 expression.Conclusion: The current study demonstrated that HPE-GNP could potentially reduce clinical and pathological complications of EAE, but laboratory data showed that HPE-GNP was significantly more effective than HPE in the treatment of EAE. |
| first_indexed | 2024-12-12T21:44:33Z |
| format | Article |
| id | doaj.art-68b48638c9ea4cebb36d2e40c0073a83 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-12T21:44:33Z |
| publishDate | 2022-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-68b48638c9ea4cebb36d2e40c0073a832022-12-22T00:10:58ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492022-05-0112332533610.22038/ajp.2022.1957419574Enhancing the efficacy of Hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles: an in vivo studyMahmoud Mahmoudi0Maryam Rastin1Mohammad Kazemi Arababadi2Akbar Anaeigoudari3Reza Nosratabadi4Immunology Research Center, Department of Immunology and Allergy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranImmunology Research Center, Department of Immunology and Allergy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Laboratory Sciences, Faculty of Paramedicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, IranDepartment of Physiology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, IranDepartment of Medical Immunology, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, IranObjective: Hypericum perforatum is a herbal medicine used in traditional medicine for the treatment of depression due to its antidepressant and anti-inflammatory activities. Therefore, we evaluated the therapeutic efficacy of H. perforatum extract (HPE) in combination with gold nanoparticles (HPE-GNP) against experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. Materials and Methods: EAE was induced in C57BL/6 mice with subcutaneous injection of MOG35-55 emulsified in complete Freund's adjuvant, and intraperitoneal pertussis toxin. Mice were treated with drugs in free (HPE) and nano-form (HPE-GNP) preparations. Splenocytes were isolated from all mice and the level of inflammatory and anti-inflammatory cytokines were evaluated by ELISA. The expression of T cells' transcription factors was also assessed using Real-Time PCR.Results: Clinical score was reduced after HPE-GNP treatment. This change was associated with a decrease in the incidence and infiltration of inflammatory cells into the central nervous system. Additionally, treatment with HPE-GNP decreased the level of pro-inflammatory cytokines (IFN-γ, IL-17A and IL-6) and increased anti-inflammatory cytokines (TGF-β, IL-10 and IL-4). The real-time analysis revealed a decrease in the level of T-bet and ROR-γt but an increase in FoxP3 and GATA3 expression.Conclusion: The current study demonstrated that HPE-GNP could potentially reduce clinical and pathological complications of EAE, but laboratory data showed that HPE-GNP was significantly more effective than HPE in the treatment of EAE.https://ajp.mums.ac.ir/article_19574_d8ef1a0c3ada92f1ac801aedf2a989c2.pdfhypericum perforatum lmultiple sclerosisexperimental autoimmuneencephalomyelitisgold nanoparticlemyelin oligodendrocyteglycoprotein |
| spellingShingle | Mahmoud Mahmoudi Maryam Rastin Mohammad Kazemi Arababadi Akbar Anaeigoudari Reza Nosratabadi Enhancing the efficacy of Hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles: an in vivo study Avicenna Journal of Phytomedicine hypericum perforatum l multiple sclerosis experimental autoimmune encephalomyelitis gold nanoparticle myelin oligodendrocyte glycoprotein |
| title | Enhancing the efficacy of Hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles: an in vivo study |
| title_full | Enhancing the efficacy of Hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles: an in vivo study |
| title_fullStr | Enhancing the efficacy of Hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles: an in vivo study |
| title_full_unstemmed | Enhancing the efficacy of Hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles: an in vivo study |
| title_short | Enhancing the efficacy of Hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles: an in vivo study |
| title_sort | enhancing the efficacy of hypericum perforatum in the treatment of an experimental model of multiple sclerosis using gold nanoparticles an in vivo study |
| topic | hypericum perforatum l multiple sclerosis experimental autoimmune encephalomyelitis gold nanoparticle myelin oligodendrocyte glycoprotein |
| url | https://ajp.mums.ac.ir/article_19574_d8ef1a0c3ada92f1ac801aedf2a989c2.pdf |
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