In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies
The implantation of oligodendrocyte precursor cells may be a useful therapeutic strategy for targeting remyelination. However, it is yet to be established how these cells behave after implantation and whether they retain the capacity to proliferate or differentiate into myelin-forming oligodendrocyt...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2073-4409/12/11/1515 |
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| author | Ulises Gómez-Pinedo Jordi A. Matías-Guiu Denise Ojeda-Hernandez Sarah de la Fuente-Martin Ola Mohamed-Fathy Kamal Maria Soledad Benito-Martin Belen Selma-Calvo Paloma Montero-Escribano Jorge Matías-Guiu |
| author_facet | Ulises Gómez-Pinedo Jordi A. Matías-Guiu Denise Ojeda-Hernandez Sarah de la Fuente-Martin Ola Mohamed-Fathy Kamal Maria Soledad Benito-Martin Belen Selma-Calvo Paloma Montero-Escribano Jorge Matías-Guiu |
| author_sort | Ulises Gómez-Pinedo |
| collection | DOAJ |
| description | The implantation of oligodendrocyte precursor cells may be a useful therapeutic strategy for targeting remyelination. However, it is yet to be established how these cells behave after implantation and whether they retain the capacity to proliferate or differentiate into myelin-forming oligodendrocytes. One essential issue is the creation of administration protocols and determining which factors need to be well established. There is controversy around whether these cells may be implanted simultaneously with corticosteroid treatment, which is widely used in many clinical situations. This study assesses the influence of corticosteroids on the capacity for proliferation and differentiation and the survival of human oligodendroglioma cells. Our findings show that corticosteroids reduce the capacity of these cells to proliferate and to differentiate into oligodendrocytes and decrease cell survival. Thus, their effect does not favour remyelination; this is consistent with the results of studies with rodent cells. In conclusion, protocols for the administration of oligodendrocyte lineage cells with the aim of repopulating oligodendroglial niches or repairing demyelinated axons should not include corticosteroids, given the evidence that the effects of these drugs may undermine the objectives of cell transplantation. |
| first_indexed | 2024-03-11T03:09:33Z |
| format | Article |
| id | doaj.art-68b49f85671e47a0bb366570e91442d3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T03:09:33Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-68b49f85671e47a0bb366570e91442d32023-11-18T07:41:07ZengMDPI AGCells2073-44092023-05-011211151510.3390/cells12111515In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell TherapiesUlises Gómez-Pinedo0Jordi A. Matías-Guiu1Denise Ojeda-Hernandez2Sarah de la Fuente-Martin3Ola Mohamed-Fathy Kamal4Maria Soledad Benito-Martin5Belen Selma-Calvo6Paloma Montero-Escribano7Jorge Matías-Guiu8Laboratory of Neurobiology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Neurology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Neurology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, IdISSC, Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainThe implantation of oligodendrocyte precursor cells may be a useful therapeutic strategy for targeting remyelination. However, it is yet to be established how these cells behave after implantation and whether they retain the capacity to proliferate or differentiate into myelin-forming oligodendrocytes. One essential issue is the creation of administration protocols and determining which factors need to be well established. There is controversy around whether these cells may be implanted simultaneously with corticosteroid treatment, which is widely used in many clinical situations. This study assesses the influence of corticosteroids on the capacity for proliferation and differentiation and the survival of human oligodendroglioma cells. Our findings show that corticosteroids reduce the capacity of these cells to proliferate and to differentiate into oligodendrocytes and decrease cell survival. Thus, their effect does not favour remyelination; this is consistent with the results of studies with rodent cells. In conclusion, protocols for the administration of oligodendrocyte lineage cells with the aim of repopulating oligodendroglial niches or repairing demyelinated axons should not include corticosteroids, given the evidence that the effects of these drugs may undermine the objectives of cell transplantation.https://www.mdpi.com/2073-4409/12/11/1515multiple sclerosisHOG cellsoligodendrocyte precursor cellsoligodendrocytescorticosteroidsdemyelination |
| spellingShingle | Ulises Gómez-Pinedo Jordi A. Matías-Guiu Denise Ojeda-Hernandez Sarah de la Fuente-Martin Ola Mohamed-Fathy Kamal Maria Soledad Benito-Martin Belen Selma-Calvo Paloma Montero-Escribano Jorge Matías-Guiu In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies Cells multiple sclerosis HOG cells oligodendrocyte precursor cells oligodendrocytes corticosteroids demyelination |
| title | In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies |
| title_full | In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies |
| title_fullStr | In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies |
| title_full_unstemmed | In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies |
| title_short | In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies |
| title_sort | in vitro effects of methylprednisolone over oligodendroglial cells foresight to future cell therapies |
| topic | multiple sclerosis HOG cells oligodendrocyte precursor cells oligodendrocytes corticosteroids demyelination |
| url | https://www.mdpi.com/2073-4409/12/11/1515 |
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