The significance of MGMT protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapy
The aim of this article was to investigate the correlation between expression of O6-methylguanine-DNA methyltransferase (MGMT) in osteosarcoma and the curative effect of alkylating agent (Cis-diaminodichloroplatinum, CDDP). 42 male patients and 34 female patients with a median age of 17 years (9 to...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2011-05-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2585 |
| _version_ | 1797260824807473152 |
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| author | Qiu Cui Dingfeng Li Cheng Liu Jun Guo Shubin Liu Yaosheng Liu Xiaohong Wang Yanjun Zeng |
| author_facet | Qiu Cui Dingfeng Li Cheng Liu Jun Guo Shubin Liu Yaosheng Liu Xiaohong Wang Yanjun Zeng |
| author_sort | Qiu Cui |
| collection | DOAJ |
| description |
The aim of this article was to investigate the correlation between expression of O6-methylguanine-DNA methyltransferase (MGMT) in osteosarcoma and the curative effect of alkylating agent (Cis-diaminodichloroplatinum, CDDP). 42 male patients and 34 female patients with a median age of 17 years (9 to 43 years) were eligible for this study. According to histopathological types, there were 3 cases of telangiectatic osteogenic sarcoma, 22 cases of osteoblastic, 11 cases of chondroblastic and 16 cases of fibroblastic sarcoma. Immunohistochemical method was used to detect the expression of MGMT protein. The correlations between MGMT expression and the curative effect of CDDP on osteosarcoma have been investigated. It was shown by immunohistochemical staining that among 76 osteosarcoma biopsy specimens, 52 (68%) cases were positive, 27 (35%) cases were weak positive, 18 (24%), cases were moderate positive, and 7 (9%) cases were strong positive. There were no significant differences in MGMT expression among different pathological types of tumors (p>0.5). After CDDP chemotherapy, among pathologic specimens in which MGMT expression was positive, necrosis rates were as follows: grade I, 5 cases (38%); grade II, 7 cases (25%); grade III, 15 cases (21%); grade IV, 2 cases (23%). Osteosarcoma necrosis rate was low when the expression of MGMT protein was positive, whereas necrosis rate was high when there was a low level of MGMT expression (p<0.01). There was a significant negative correlation between the level of MGMT expression in osteosarcoma tissue and osteosarcoma necrosis rate after cisplatin chemotherapy.
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| first_indexed | 2024-04-24T23:31:28Z |
| format | Article |
| id | doaj.art-68b592d2f52b4bf39263705236b2b9a6 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:31:28Z |
| publishDate | 2011-05-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-68b592d2f52b4bf39263705236b2b9a62024-03-15T14:34:58ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2011-05-0111210.17305/bjbms.2011.2585345The significance of MGMT protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapyQiu Cui0Dingfeng Li1Cheng Liu2Jun Guo3Shubin Liu4Yaosheng Liu5Xiaohong Wang6Yanjun Zeng7Department of Orthopedics, the Affiliated Hospital of Academy of Military Medical Sciences, PLA 307th HospitalDepartment of Orthopedics, the Affiliated Hospital of Academy of Military Medical Sciences, PLA 307th HospitalDepartment of Orthopedics, the Affiliated Hospital of Academy of Military Medical Sciences, PLA 307th HospitalDepartment of Orthopedics, the Affiliated Hospital of Academy of Military Medical Sciences, PLA 307th HospitalDepartment of Orthopedics, the Affiliated Hospital of Academy of Military Medical Sciences, PLA 307th HospitalDepartment of Orthopedics, the Affiliated Hospital of Academy of Military Medical Sciences, PLA 307th HospitalDepartment of Orthopedics, the Affiliated Hospital of Academy of Military Medical Sciences, PLA 307th HospitalBiomechanics and Medical Information Institute, Beijing University of Technology The aim of this article was to investigate the correlation between expression of O6-methylguanine-DNA methyltransferase (MGMT) in osteosarcoma and the curative effect of alkylating agent (Cis-diaminodichloroplatinum, CDDP). 42 male patients and 34 female patients with a median age of 17 years (9 to 43 years) were eligible for this study. According to histopathological types, there were 3 cases of telangiectatic osteogenic sarcoma, 22 cases of osteoblastic, 11 cases of chondroblastic and 16 cases of fibroblastic sarcoma. Immunohistochemical method was used to detect the expression of MGMT protein. The correlations between MGMT expression and the curative effect of CDDP on osteosarcoma have been investigated. It was shown by immunohistochemical staining that among 76 osteosarcoma biopsy specimens, 52 (68%) cases were positive, 27 (35%) cases were weak positive, 18 (24%), cases were moderate positive, and 7 (9%) cases were strong positive. There were no significant differences in MGMT expression among different pathological types of tumors (p>0.5). After CDDP chemotherapy, among pathologic specimens in which MGMT expression was positive, necrosis rates were as follows: grade I, 5 cases (38%); grade II, 7 cases (25%); grade III, 15 cases (21%); grade IV, 2 cases (23%). Osteosarcoma necrosis rate was low when the expression of MGMT protein was positive, whereas necrosis rate was high when there was a low level of MGMT expression (p<0.01). There was a significant negative correlation between the level of MGMT expression in osteosarcoma tissue and osteosarcoma necrosis rate after cisplatin chemotherapy. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2585osteosarcomaMGMT proteinCDDP |
| spellingShingle | Qiu Cui Dingfeng Li Cheng Liu Jun Guo Shubin Liu Yaosheng Liu Xiaohong Wang Yanjun Zeng The significance of MGMT protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapy Biomolecules & Biomedicine osteosarcoma MGMT protein CDDP |
| title | The significance of MGMT protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapy |
| title_full | The significance of MGMT protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapy |
| title_fullStr | The significance of MGMT protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapy |
| title_full_unstemmed | The significance of MGMT protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapy |
| title_short | The significance of MGMT protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapy |
| title_sort | significance of mgmt protein detection in evaluation of osteosarcoma necrosis rate after cisplatin chemotherapy |
| topic | osteosarcoma MGMT protein CDDP |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2585 |
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