Encapsulating Anti-Parasite Benzimidazole Drugs into Lipid-Coated Calcium Phosphate Nanoparticles to Efficiently Induce Skin Cancer Cell Apoptosis
Benzimidazole (BMZ) family of anti-worm drugs has been now repurposed as anti-cancer drugs. However, offering a general reformulation method for these drugs is essential due to their hydrophobicity and low aqueous solubility. In this work, we developed a general approach to load typical BMZ drugs as...
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| Format: | Article |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Nanotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnano.2021.693837/full |
| _version_ | 1831591687794720768 |
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| author | Fatemeh Movahedi Wenyi Gu Christiane Pienna Soares Zhi Ping Xu |
| author_facet | Fatemeh Movahedi Wenyi Gu Christiane Pienna Soares Zhi Ping Xu |
| author_sort | Fatemeh Movahedi |
| collection | DOAJ |
| description | Benzimidazole (BMZ) family of anti-worm drugs has been now repurposed as anti-cancer drugs. However, offering a general reformulation method for these drugs is essential due to their hydrophobicity and low aqueous solubility. In this work, we developed a general approach to load typical BMZ drugs as tiny nanocrystals within lipid-coated calcium phosphate (LCP) nanoparticles. BMZ drug-loaded LCP nanoparticles increased their solubility in PBS by 100–200% and significantly enhanced the anti-cancer efficacy in the treatment of B16F0 melanoma cells. These drug-LCP nanoparticles induced much more cancer cell apoptosis, generated much more reactive oxygen species (ROS) and inhibited Bcl-2 expression of cancer cells. Moreover, BMZ drug-loaded LCP nanoparticles caused morphological change and extension disruption of cancer cells, and significantly reduced migration activity, representing high possibility for inhibition of tumor dissemination and metastasis. Very advantageously, BMZ drug-loaded LCP nanoparticles did not show any obvious toxicity, Bcl-2 inhibition and morphological changes in HEK293T healthy cells. In conclusion, BMZ drug-incorporated LCP nanoformulations may be a valuable nanomedicine that is able to inhibit primary tumors and prevent tumor dissemination with minimum side effects on healthy cells and tissues. |
| first_indexed | 2024-12-18T01:29:41Z |
| format | Article |
| id | doaj.art-68b5aeef035f4dcdbab0a4508fa6801a |
| institution | Directory Open Access Journal |
| issn | 2673-3013 |
| language | English |
| last_indexed | 2024-12-18T01:29:41Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nanotechnology |
| spelling | doaj.art-68b5aeef035f4dcdbab0a4508fa6801a2022-12-21T21:25:38ZengFrontiers Media S.A.Frontiers in Nanotechnology2673-30132021-06-01310.3389/fnano.2021.693837693837Encapsulating Anti-Parasite Benzimidazole Drugs into Lipid-Coated Calcium Phosphate Nanoparticles to Efficiently Induce Skin Cancer Cell ApoptosisFatemeh Movahedi0Wenyi Gu1Christiane Pienna Soares2Zhi Ping Xu3Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St Lucia, QLD, AustraliaAustralian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St Lucia, QLD, AustraliaDepartment of Clinical Analysis, School of Pharmaceutical Sciences, Universidade Estadual Paulista (UNESP), Araraquara, BrazilAustralian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St Lucia, QLD, AustraliaBenzimidazole (BMZ) family of anti-worm drugs has been now repurposed as anti-cancer drugs. However, offering a general reformulation method for these drugs is essential due to their hydrophobicity and low aqueous solubility. In this work, we developed a general approach to load typical BMZ drugs as tiny nanocrystals within lipid-coated calcium phosphate (LCP) nanoparticles. BMZ drug-loaded LCP nanoparticles increased their solubility in PBS by 100–200% and significantly enhanced the anti-cancer efficacy in the treatment of B16F0 melanoma cells. These drug-LCP nanoparticles induced much more cancer cell apoptosis, generated much more reactive oxygen species (ROS) and inhibited Bcl-2 expression of cancer cells. Moreover, BMZ drug-loaded LCP nanoparticles caused morphological change and extension disruption of cancer cells, and significantly reduced migration activity, representing high possibility for inhibition of tumor dissemination and metastasis. Very advantageously, BMZ drug-loaded LCP nanoparticles did not show any obvious toxicity, Bcl-2 inhibition and morphological changes in HEK293T healthy cells. In conclusion, BMZ drug-incorporated LCP nanoformulations may be a valuable nanomedicine that is able to inhibit primary tumors and prevent tumor dissemination with minimum side effects on healthy cells and tissues.https://www.frontiersin.org/articles/10.3389/fnano.2021.693837/fullbenzimidazole family of anti-worm drugslipid-coated calcium phosphate nanoparticlesenhanced solubilityenhanced anti-cancer toxicityinhibition of cancer dissemination |
| spellingShingle | Fatemeh Movahedi Wenyi Gu Christiane Pienna Soares Zhi Ping Xu Encapsulating Anti-Parasite Benzimidazole Drugs into Lipid-Coated Calcium Phosphate Nanoparticles to Efficiently Induce Skin Cancer Cell Apoptosis Frontiers in Nanotechnology benzimidazole family of anti-worm drugs lipid-coated calcium phosphate nanoparticles enhanced solubility enhanced anti-cancer toxicity inhibition of cancer dissemination |
| title | Encapsulating Anti-Parasite Benzimidazole Drugs into Lipid-Coated Calcium Phosphate Nanoparticles to Efficiently Induce Skin Cancer Cell Apoptosis |
| title_full | Encapsulating Anti-Parasite Benzimidazole Drugs into Lipid-Coated Calcium Phosphate Nanoparticles to Efficiently Induce Skin Cancer Cell Apoptosis |
| title_fullStr | Encapsulating Anti-Parasite Benzimidazole Drugs into Lipid-Coated Calcium Phosphate Nanoparticles to Efficiently Induce Skin Cancer Cell Apoptosis |
| title_full_unstemmed | Encapsulating Anti-Parasite Benzimidazole Drugs into Lipid-Coated Calcium Phosphate Nanoparticles to Efficiently Induce Skin Cancer Cell Apoptosis |
| title_short | Encapsulating Anti-Parasite Benzimidazole Drugs into Lipid-Coated Calcium Phosphate Nanoparticles to Efficiently Induce Skin Cancer Cell Apoptosis |
| title_sort | encapsulating anti parasite benzimidazole drugs into lipid coated calcium phosphate nanoparticles to efficiently induce skin cancer cell apoptosis |
| topic | benzimidazole family of anti-worm drugs lipid-coated calcium phosphate nanoparticles enhanced solubility enhanced anti-cancer toxicity inhibition of cancer dissemination |
| url | https://www.frontiersin.org/articles/10.3389/fnano.2021.693837/full |
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