Homeostasis of metabolites in Escherichia coli on transition from anaerobic to aerobic conditions and the transient secretion of pyruvate
We have developed a method for rapid quenching of samples taken from chemostat cultures of Escherichia coli that gives reproducible and reliable measurements of extracellular and intracellular metabolites by 1H NMR and have applied it to study the major central metabolites during the transition from...
Main Authors: | Nur Adeela Yasid, Matthew D. Rolfe, Jeffrey Green, Mike P. Williamson |
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Format: | Article |
Language: | English |
Published: |
The Royal Society
2016-01-01
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Series: | Royal Society Open Science |
Subjects: | |
Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160187 |
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