Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil
The keratin degrading bacterial strain KRD1 was isolated from poultry farm soil by using enrichment technology. The strain was identified as Bacillus olironius on the basis of biochemical and 16s rRNA sequencing. The keratinase production was maximum in keratin medium under optimum condition (pH 7.5...
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Format: | Article |
Language: | English |
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Journal of Pure and Applied Microbiology
2017-06-01
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Series: | Journal of Pure and Applied Microbiology |
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Online Access: | https://microbiologyjournal.org/optimization-of-keratinase-production-by-bacillus-olironius-isolated-from-poultry-farm-soil/ |
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author | Deepak Parashar Divya Bhatia Deepak Kumar Malik |
author_facet | Deepak Parashar Divya Bhatia Deepak Kumar Malik |
author_sort | Deepak Parashar |
collection | DOAJ |
description | The keratin degrading bacterial strain KRD1 was isolated from poultry farm soil by using enrichment technology. The strain was identified as Bacillus olironius on the basis of biochemical and 16s rRNA sequencing. The keratinase production was maximum in keratin medium under optimum condition (pH 7.5, time 96 h; temperature 37 °C; and 5 % inoculums). The KRD1 keratinase activity decreased by the addition of carbon sources and strain KRD1 produce high keratinase in the presence of 0.1 % yeast extract. Therefore enzymatic hydrolysis of feather wastes could be a safe method for recycling of keratin materials. |
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format | Article |
id | doaj.art-68b862785ae54ba89f89d5bf8f4d881d |
institution | Directory Open Access Journal |
issn | 0973-7510 2581-690X |
language | English |
last_indexed | 2024-12-17T23:08:56Z |
publishDate | 2017-06-01 |
publisher | Journal of Pure and Applied Microbiology |
record_format | Article |
series | Journal of Pure and Applied Microbiology |
spelling | doaj.art-68b862785ae54ba89f89d5bf8f4d881d2022-12-21T21:29:11ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-06-011121129113410.22207/JPAM.11.2.58Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm SoilDeepak Parashar0Divya Bhatia1Deepak Kumar Malik2Department of Biotechnology, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra - 136 119, Haryana, India.Department of Biotechnology, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra - 136 119, Haryana, India.Department of Biotechnology, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra - 136 119, Haryana, India.The keratin degrading bacterial strain KRD1 was isolated from poultry farm soil by using enrichment technology. The strain was identified as Bacillus olironius on the basis of biochemical and 16s rRNA sequencing. The keratinase production was maximum in keratin medium under optimum condition (pH 7.5, time 96 h; temperature 37 °C; and 5 % inoculums). The KRD1 keratinase activity decreased by the addition of carbon sources and strain KRD1 produce high keratinase in the presence of 0.1 % yeast extract. Therefore enzymatic hydrolysis of feather wastes could be a safe method for recycling of keratin materials.https://microbiologyjournal.org/optimization-of-keratinase-production-by-bacillus-olironius-isolated-from-poultry-farm-soil/bacillus olironiuskeratenasepoultry soil |
spellingShingle | Deepak Parashar Divya Bhatia Deepak Kumar Malik Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil Journal of Pure and Applied Microbiology bacillus olironius keratenase poultry soil |
title | Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil |
title_full | Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil |
title_fullStr | Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil |
title_full_unstemmed | Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil |
title_short | Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil |
title_sort | optimization of keratinase production by bacillus olironius isolated from poultry farm soil |
topic | bacillus olironius keratenase poultry soil |
url | https://microbiologyjournal.org/optimization-of-keratinase-production-by-bacillus-olironius-isolated-from-poultry-farm-soil/ |
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