Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil

The keratin degrading bacterial strain KRD1 was isolated from poultry farm soil by using enrichment technology. The strain was identified as Bacillus olironius on the basis of biochemical and 16s rRNA sequencing. The keratinase production was maximum in keratin medium under optimum condition (pH 7.5...

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Main Authors: Deepak Parashar, Divya Bhatia, Deepak Kumar Malik
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2017-06-01
Series:Journal of Pure and Applied Microbiology
Subjects:
Online Access:https://microbiologyjournal.org/optimization-of-keratinase-production-by-bacillus-olironius-isolated-from-poultry-farm-soil/
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author Deepak Parashar
Divya Bhatia
Deepak Kumar Malik
author_facet Deepak Parashar
Divya Bhatia
Deepak Kumar Malik
author_sort Deepak Parashar
collection DOAJ
description The keratin degrading bacterial strain KRD1 was isolated from poultry farm soil by using enrichment technology. The strain was identified as Bacillus olironius on the basis of biochemical and 16s rRNA sequencing. The keratinase production was maximum in keratin medium under optimum condition (pH 7.5, time 96 h; temperature 37 °C; and 5 % inoculums). The KRD1 keratinase activity decreased by the addition of carbon sources and strain KRD1 produce high keratinase in the presence of 0.1 % yeast extract. Therefore enzymatic hydrolysis of feather wastes could be a safe method for recycling of keratin materials.
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spelling doaj.art-68b862785ae54ba89f89d5bf8f4d881d2022-12-21T21:29:11ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-06-011121129113410.22207/JPAM.11.2.58Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm SoilDeepak Parashar0Divya Bhatia1Deepak Kumar Malik2Department of Biotechnology, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra - 136 119, Haryana, India.Department of Biotechnology, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra - 136 119, Haryana, India.Department of Biotechnology, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra - 136 119, Haryana, India.The keratin degrading bacterial strain KRD1 was isolated from poultry farm soil by using enrichment technology. The strain was identified as Bacillus olironius on the basis of biochemical and 16s rRNA sequencing. The keratinase production was maximum in keratin medium under optimum condition (pH 7.5, time 96 h; temperature 37 °C; and 5 % inoculums). The KRD1 keratinase activity decreased by the addition of carbon sources and strain KRD1 produce high keratinase in the presence of 0.1 % yeast extract. Therefore enzymatic hydrolysis of feather wastes could be a safe method for recycling of keratin materials.https://microbiologyjournal.org/optimization-of-keratinase-production-by-bacillus-olironius-isolated-from-poultry-farm-soil/bacillus olironiuskeratenasepoultry soil
spellingShingle Deepak Parashar
Divya Bhatia
Deepak Kumar Malik
Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil
Journal of Pure and Applied Microbiology
bacillus olironius
keratenase
poultry soil
title Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil
title_full Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil
title_fullStr Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil
title_full_unstemmed Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil
title_short Optimization of Keratinase Production by Bacillus olironius Isolated from Poultry Farm Soil
title_sort optimization of keratinase production by bacillus olironius isolated from poultry farm soil
topic bacillus olironius
keratenase
poultry soil
url https://microbiologyjournal.org/optimization-of-keratinase-production-by-bacillus-olironius-isolated-from-poultry-farm-soil/
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