Age-Dependent Changes in the mRNA Levels of Neuropeptide Y, Proopiomelanocortin, and Corticotropin-Releasing Factor in the Hypothalamus in Growing Broiler Chicks
Lines of evidence suggest that hypothalamic neuropeptides have a physiological role in the regulation of food intake in mammals. However, little is known about the age-dependent change of hypothalamic neuropeptides in chicks. In this study, we investigated the age-dependent changes in hypothalamic...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2013-10-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/50/4/50_0120188/_pdf/-char/en |
| _version_ | 1797816181641445376 |
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| author | Takaoki Saneyasu Kiwako Nakanishi Hiroyuki Atsuta Atsushi Ikura Hiroshi Kamisoyama Shin Hasegawa Kazuhisa Honda |
| author_facet | Takaoki Saneyasu Kiwako Nakanishi Hiroyuki Atsuta Atsushi Ikura Hiroshi Kamisoyama Shin Hasegawa Kazuhisa Honda |
| author_sort | Takaoki Saneyasu |
| collection | DOAJ |
| description | Lines of evidence suggest that hypothalamic neuropeptides have a physiological role in the regulation of food intake in mammals. However, little is known about the age-dependent change of hypothalamic neuropeptides in chicks. In this study, we investigated the age-dependent changes in hypothalamic mRNA levels of neuropeptide Y (NPY, an orexigenic neuropeptide), proopiomelanocortin (POMC, the precursor of anorexigenic neuropeptides), and corticotropin-releasing factor (CRF, a possible mediator in the POMC-induced anorexigenic pathway) in growing broiler chicks. The weights of body and abdominal adipose tissue of chicks were measured at 7, 14, 21, and 28 days of age, and the hypothalamic mRNA levels of NPY, POMC, and CRF were analyzed by real-time PCR. Hypothalamic NPY mRNA levels at 14 and 28 days of age were significantly (P<0.05) lower than that at 7 days of age. Hypothalamic POMC mRNA level at 28 days of age was significantly lower than that at 7 days of age. Hypothalamic CRF mRNA level was not affected throughout the experimental period. Abdominal adipose tissue weight was significantly increased after 14 days of age in chicks. Our findings demonstrated that the expressions of NPY and POMC are dependent on age in boiler chicken hypothalamus. |
| first_indexed | 2024-03-13T08:33:57Z |
| format | Article |
| id | doaj.art-68bc5bd53620491c8d54c6300a494c9d |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:33:57Z |
| publishDate | 2013-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-68bc5bd53620491c8d54c6300a494c9d2023-05-31T01:28:36ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862013-10-0150436436910.2141/jpsa.0120188jpsaAge-Dependent Changes in the mRNA Levels of Neuropeptide Y, Proopiomelanocortin, and Corticotropin-Releasing Factor in the Hypothalamus in Growing Broiler ChicksTakaoki Saneyasu0Kiwako Nakanishi1Hiroyuki Atsuta2Atsushi Ikura3Hiroshi Kamisoyama4Shin Hasegawa5Kazuhisa Honda6Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, JapanDepartment of Bioresource Science, Graduate School of Agricultural Science, Kobe University, JapanDepartment of Bioresource Science, Graduate School of Agricultural Science, Kobe University, JapanDepartment of Bioresource Science, Graduate School of Agricultural Science, Kobe University, JapanDepartment of Bioresource Science, Graduate School of Agricultural Science, Kobe University, JapanDepartment of Bioresource Science, Graduate School of Agricultural Science, Kobe University, JapanDepartment of Bioresource Science, Graduate School of Agricultural Science, Kobe University, JapanLines of evidence suggest that hypothalamic neuropeptides have a physiological role in the regulation of food intake in mammals. However, little is known about the age-dependent change of hypothalamic neuropeptides in chicks. In this study, we investigated the age-dependent changes in hypothalamic mRNA levels of neuropeptide Y (NPY, an orexigenic neuropeptide), proopiomelanocortin (POMC, the precursor of anorexigenic neuropeptides), and corticotropin-releasing factor (CRF, a possible mediator in the POMC-induced anorexigenic pathway) in growing broiler chicks. The weights of body and abdominal adipose tissue of chicks were measured at 7, 14, 21, and 28 days of age, and the hypothalamic mRNA levels of NPY, POMC, and CRF were analyzed by real-time PCR. Hypothalamic NPY mRNA levels at 14 and 28 days of age were significantly (P<0.05) lower than that at 7 days of age. Hypothalamic POMC mRNA level at 28 days of age was significantly lower than that at 7 days of age. Hypothalamic CRF mRNA level was not affected throughout the experimental period. Abdominal adipose tissue weight was significantly increased after 14 days of age in chicks. Our findings demonstrated that the expressions of NPY and POMC are dependent on age in boiler chicken hypothalamus.https://www.jstage.jst.go.jp/article/jpsa/50/4/50_0120188/_pdf/-char/enappetitecrfhypothalamusnpypomc |
| spellingShingle | Takaoki Saneyasu Kiwako Nakanishi Hiroyuki Atsuta Atsushi Ikura Hiroshi Kamisoyama Shin Hasegawa Kazuhisa Honda Age-Dependent Changes in the mRNA Levels of Neuropeptide Y, Proopiomelanocortin, and Corticotropin-Releasing Factor in the Hypothalamus in Growing Broiler Chicks The Journal of Poultry Science appetite crf hypothalamus npy pomc |
| title | Age-Dependent Changes in the mRNA Levels of Neuropeptide Y, Proopiomelanocortin, and Corticotropin-Releasing Factor in the Hypothalamus in Growing Broiler Chicks |
| title_full | Age-Dependent Changes in the mRNA Levels of Neuropeptide Y, Proopiomelanocortin, and Corticotropin-Releasing Factor in the Hypothalamus in Growing Broiler Chicks |
| title_fullStr | Age-Dependent Changes in the mRNA Levels of Neuropeptide Y, Proopiomelanocortin, and Corticotropin-Releasing Factor in the Hypothalamus in Growing Broiler Chicks |
| title_full_unstemmed | Age-Dependent Changes in the mRNA Levels of Neuropeptide Y, Proopiomelanocortin, and Corticotropin-Releasing Factor in the Hypothalamus in Growing Broiler Chicks |
| title_short | Age-Dependent Changes in the mRNA Levels of Neuropeptide Y, Proopiomelanocortin, and Corticotropin-Releasing Factor in the Hypothalamus in Growing Broiler Chicks |
| title_sort | age dependent changes in the mrna levels of neuropeptide y proopiomelanocortin and corticotropin releasing factor in the hypothalamus in growing broiler chicks |
| topic | appetite crf hypothalamus npy pomc |
| url | https://www.jstage.jst.go.jp/article/jpsa/50/4/50_0120188/_pdf/-char/en |
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