In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions.
Stressosomes are signal-sensing and integration hubs identified in many bacteria. At present, the role of the stressosome has only been investigated in Gram-positive bacteria. This work represents the first in vivo characterisation of the stressosome in a Gram-negative bacterium, Vibrio vulnificus....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Microbial Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266651742300007X |
| _version_ | 1797800976883646464 |
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| author | Laura Cutugno Borja Khatabi Soliman Tamayo Piet N.L. Lens Conor O'Byrne Jan Pané-Farré Aoife Boyd |
| author_facet | Laura Cutugno Borja Khatabi Soliman Tamayo Piet N.L. Lens Conor O'Byrne Jan Pané-Farré Aoife Boyd |
| author_sort | Laura Cutugno |
| collection | DOAJ |
| description | Stressosomes are signal-sensing and integration hubs identified in many bacteria. At present, the role of the stressosome has only been investigated in Gram-positive bacteria. This work represents the first in vivo characterisation of the stressosome in a Gram-negative bacterium, Vibrio vulnificus. Previous in vitro characterisation of the complex has led to the hypothesis of a complex involved in iron metabolism and control of c-di-GMP levels. We demonstrate that the stressosome is probably involved in reshaping the glucose metabolism in Fe- and nutrient-limited conditions and mutations of the locus affect the activation of the glyoxylate shunt. Moreover, we show that the stressosome is needed for the transcription of fleQ and to promote motility, consistent with the hypothesis that the stressosome is involved in regulating c-di-GMP. This report highlights the potential role of the stressosome in a Gram-negative bacterium, with implications for the metabolism and motility of this pathogen. |
| first_indexed | 2024-03-13T04:43:24Z |
| format | Article |
| id | doaj.art-68bcd89b9976422795c19807f83b131f |
| institution | Directory Open Access Journal |
| issn | 2666-5174 |
| language | English |
| last_indexed | 2024-03-13T04:43:24Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj.art-68bcd89b9976422795c19807f83b131f2023-06-19T04:29:42ZengElsevierCurrent Research in Microbial Sciences2666-51742023-01-014100186In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions.Laura Cutugno0Borja Khatabi Soliman Tamayo1Piet N.L. Lens2Conor O'Byrne3Jan Pané-Farré4Aoife Boyd5School of Natural Sciences, University of Galway, IrelandSchool of Biological and Chemical Sciences, University of Galway, IrelandSchool of Biological and Chemical Sciences, University of Galway, IrelandSchool of Biological and Chemical Sciences, University of Galway, IrelandCentre for synthetic Microbiology (SYNMIKRO) & Department of Chemistry, Philipps-University Marburg, GermanySchool of Natural Sciences, University of Galway, Ireland; Corresponding author.Stressosomes are signal-sensing and integration hubs identified in many bacteria. At present, the role of the stressosome has only been investigated in Gram-positive bacteria. This work represents the first in vivo characterisation of the stressosome in a Gram-negative bacterium, Vibrio vulnificus. Previous in vitro characterisation of the complex has led to the hypothesis of a complex involved in iron metabolism and control of c-di-GMP levels. We demonstrate that the stressosome is probably involved in reshaping the glucose metabolism in Fe- and nutrient-limited conditions and mutations of the locus affect the activation of the glyoxylate shunt. Moreover, we show that the stressosome is needed for the transcription of fleQ and to promote motility, consistent with the hypothesis that the stressosome is involved in regulating c-di-GMP. This report highlights the potential role of the stressosome in a Gram-negative bacterium, with implications for the metabolism and motility of this pathogen.http://www.sciencedirect.com/science/article/pii/S266651742300007XVibrio vulnificusStress responseStressosomeIron metabolismVirulenceMotility |
| spellingShingle | Laura Cutugno Borja Khatabi Soliman Tamayo Piet N.L. Lens Conor O'Byrne Jan Pané-Farré Aoife Boyd In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. Current Research in Microbial Sciences Vibrio vulnificus Stress response Stressosome Iron metabolism Virulence Motility |
| title | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
| title_full | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
| title_fullStr | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
| title_full_unstemmed | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
| title_short | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
| title_sort | in vivo characterisation of the vibrio vulnificus stressosome a complex involved in reshaping glucose metabolism and motility regulation in nutrient and iron limited growth conditions |
| topic | Vibrio vulnificus Stress response Stressosome Iron metabolism Virulence Motility |
| url | http://www.sciencedirect.com/science/article/pii/S266651742300007X |
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