MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1
MicroRNA-29a (miR-29a) has been extensively studied in tumor biology and fibrotic diseases, but little is known about its functional roles in vascular smooth muscle cell (VSMC) differentiation from embryonic stem cells (ESCs). Using well-established VSMC differentiation models, we have observed that...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-09-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350611630099X |
| _version_ | 1818066437759041536 |
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| author | Min Jin Yutao Wu Yanwei Wang Danqing Yu Mei Yang Feng Yang Chun Feng Ting Chen |
| author_facet | Min Jin Yutao Wu Yanwei Wang Danqing Yu Mei Yang Feng Yang Chun Feng Ting Chen |
| author_sort | Min Jin |
| collection | DOAJ |
| description | MicroRNA-29a (miR-29a) has been extensively studied in tumor biology and fibrotic diseases, but little is known about its functional roles in vascular smooth muscle cell (VSMC) differentiation from embryonic stem cells (ESCs). Using well-established VSMC differentiation models, we have observed that miR-29a induces VSMC differentiation from mouse ESCs by negatively regulating YY1, a transcription factor that inhibits muscle cell differentiation and muscle-specific gene expression. Moreover, gene expression levels of three VSMC specific transcriptional factors were up-regulated by miR-29a over-expression, but down-regulated by miR-29a inhibition or YY1 over-expression. Taken together, our data demonstrate that miR-29a and its target gene, YY1, play a regulatory role in VSMC differentiation from ESCs in vitro and in vivo. |
| first_indexed | 2024-12-10T15:07:47Z |
| format | Article |
| id | doaj.art-68bd1c38e7eb4ce98a76afe068490809 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-12-10T15:07:47Z |
| publishDate | 2016-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-68bd1c38e7eb4ce98a76afe0684908092022-12-22T01:44:00ZengElsevierStem Cell Research1873-50611876-77532016-09-0117227728410.1016/j.scr.2016.07.011MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1Min Jin0Yutao Wu1Yanwei Wang2Danqing Yu3Mei Yang4Feng Yang5Chun Feng6Ting Chen7Division of Reproductive Medicine & Infertility, The Second Affiliated Hospital, School of Medicine, Zhejiang University, 88#, Jiefang Rd., Hangzhou, Zhejiang 310009, PR ChinaDepartment of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR ChinaDepartment of Cardiology, Ningbo Medical Treatment Center Lihuili Hospital, Ningbo 315000, PR ChinaDivision of Reproductive Medicine & Infertility, The Second Affiliated Hospital, School of Medicine, Zhejiang University, 88#, Jiefang Rd., Hangzhou, Zhejiang 310009, PR ChinaDepartment of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR ChinaDepartment of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR ChinaDivision of Reproductive Medicine & Infertility, The Second Affiliated Hospital, School of Medicine, Zhejiang University, 88#, Jiefang Rd., Hangzhou, Zhejiang 310009, PR ChinaDepartment of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR ChinaMicroRNA-29a (miR-29a) has been extensively studied in tumor biology and fibrotic diseases, but little is known about its functional roles in vascular smooth muscle cell (VSMC) differentiation from embryonic stem cells (ESCs). Using well-established VSMC differentiation models, we have observed that miR-29a induces VSMC differentiation from mouse ESCs by negatively regulating YY1, a transcription factor that inhibits muscle cell differentiation and muscle-specific gene expression. Moreover, gene expression levels of three VSMC specific transcriptional factors were up-regulated by miR-29a over-expression, but down-regulated by miR-29a inhibition or YY1 over-expression. Taken together, our data demonstrate that miR-29a and its target gene, YY1, play a regulatory role in VSMC differentiation from ESCs in vitro and in vivo.http://www.sciencedirect.com/science/article/pii/S187350611630099X |
| spellingShingle | Min Jin Yutao Wu Yanwei Wang Danqing Yu Mei Yang Feng Yang Chun Feng Ting Chen MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1 Stem Cell Research |
| title | MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1 |
| title_full | MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1 |
| title_fullStr | MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1 |
| title_full_unstemmed | MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1 |
| title_short | MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1 |
| title_sort | microrna 29a promotes smooth muscle cell differentiation from stem cells by targeting yy1 |
| url | http://www.sciencedirect.com/science/article/pii/S187350611630099X |
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