Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis
Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/7/755 |
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| author | Rima Budvytyte Akvile Milasiute Dalius Vitkus Kestutis Strupas Aiste Gulla Ieva Sakinyte Julija Razumiene |
| author_facet | Rima Budvytyte Akvile Milasiute Dalius Vitkus Kestutis Strupas Aiste Gulla Ieva Sakinyte Julija Razumiene |
| author_sort | Rima Budvytyte |
| collection | DOAJ |
| description | Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity. |
| first_indexed | 2024-03-09T04:44:56Z |
| format | Article |
| id | doaj.art-68bf700891074b658839e70a62fbe4a2 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T04:44:56Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-68bf700891074b658839e70a62fbe4a22023-12-03T13:16:58ZengMDPI AGBiomedicines2227-90592021-06-019775510.3390/biomedicines9070755Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute PancreatitisRima Budvytyte0Akvile Milasiute1Dalius Vitkus2Kestutis Strupas3Aiste Gulla4Ieva Sakinyte5Julija Razumiene6Life Sciences Center, Institute of Biochemistry, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaLife Sciences Center, Institute of Biochemistry, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaInstitute of Biomedical Sciences, Faculty of Medicine, Vilnius University, M.K.Ciurlionio St. 21, LT-03101 Vilnius, LithuaniaInstitute of Clinical Medicine, Faculty of Medicine, Vilnius University, M.K.Ciurlionio St. 21, LT-03101 Vilnius, LithuaniaInstitute of Clinical Medicine, Faculty of Medicine, Vilnius University, M.K.Ciurlionio St. 21, LT-03101 Vilnius, LithuaniaLife Sciences Center, Institute of Biochemistry, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaLife Sciences Center, Institute of Biochemistry, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaExtracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.https://www.mdpi.com/2227-9059/9/7/755HSP70 and HSP90severity of acute pancreatitistethered bilayer lipid membraneselectrochemical impedance spectroscopyurine |
| spellingShingle | Rima Budvytyte Akvile Milasiute Dalius Vitkus Kestutis Strupas Aiste Gulla Ieva Sakinyte Julija Razumiene Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis Biomedicines HSP70 and HSP90 severity of acute pancreatitis tethered bilayer lipid membranes electrochemical impedance spectroscopy urine |
| title | Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis |
| title_full | Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis |
| title_fullStr | Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis |
| title_full_unstemmed | Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis |
| title_short | Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis |
| title_sort | tethered lipid membranes as a nanoscale arrangement towards non invasive analysis of acute pancreatitis |
| topic | HSP70 and HSP90 severity of acute pancreatitis tethered bilayer lipid membranes electrochemical impedance spectroscopy urine |
| url | https://www.mdpi.com/2227-9059/9/7/755 |
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