Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq
The target of this study was to study the natural phytochemical components of the head (capsule) of Cynara scolymus cultivated in Iraq. The head (capsule) of plant was extracted by maceration in70% ethanol for 72 hours, and fractioned by hexane, chloroform and ethyl acetate. Preliminary qualitative...
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| Format: | Article |
| Language: | English |
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College of Pharmacy University of Baghdad
2020-12-01
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| Series: | Iraqi Journal of Pharmaceutical Sciences |
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| Online Access: | https://www.bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1076 |
| _version_ | 1797317320100544512 |
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| author | Zaineb H. Ajeel Maha N. Hamad |
| author_facet | Zaineb H. Ajeel Maha N. Hamad |
| author_sort | Zaineb H. Ajeel |
| collection | DOAJ |
| description |
The target of this study was to study the natural phytochemical components of the head (capsule) of Cynara scolymus cultivated in Iraq. The head (capsule) of plant was extracted by maceration in70% ethanol for 72 hours, and fractioned by hexane, chloroform and ethyl acetate. Preliminary qualitative phytochemical screening was performed on the ethyl acetate fraction for capsule was revealed the presence of flavonoid and aromatic acids. These were examined by (high -performance liquid chromatography) (HPLC diodarray), (high- performance thin-layer chromatography)(HPTLC).
Flavonoids were isolated by preparative layer chromatography and aromatic acid was isolated by preparative high-performance liquid chromatography HPLC from the ethyl acetate fraction of capsule.
Then identified by High Performance Thin Layer Chromatography HPTLC, High performance liquid chromatography HPLC diode array , ultraviolet diode array UV-diode array and Liquid Chromatography /Mass Spectroscopy LC/MS. The chloroform fraction from the capsule was evaluated by Gas Chromatography//Mass Spectrometer(GC/MS). The different chromatographic and spectroscopic techniques revealed the presence of luteolin, apigenin and cinnamic acid in capsule of Cynara scolymus, also 9-octadecanoic acid (oleic acid), Oxalic acid, allyl tetradecyl ester, limonene, in chloroform of Cynara scolymus.
The results of the current study proved the presence of luleolin, apigenin, and cinnamic acid in the ethyl acetate fraction of Cynara scolymus capsule.
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| first_indexed | 2024-03-08T03:33:12Z |
| format | Article |
| id | doaj.art-68c16492c8ea49089f38981f45b6b554 |
| institution | Directory Open Access Journal |
| issn | 1683-3597 2521-3512 |
| language | English |
| last_indexed | 2024-03-08T03:33:12Z |
| publishDate | 2020-12-01 |
| publisher | College of Pharmacy University of Baghdad |
| record_format | Article |
| series | Iraqi Journal of Pharmaceutical Sciences |
| spelling | doaj.art-68c16492c8ea49089f38981f45b6b5542024-02-10T10:57:56ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences1683-35972521-35122020-12-0129210.31351/vol29iss2pp202-213Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraqZaineb H. Ajeel0Maha N. HamadWassit The target of this study was to study the natural phytochemical components of the head (capsule) of Cynara scolymus cultivated in Iraq. The head (capsule) of plant was extracted by maceration in70% ethanol for 72 hours, and fractioned by hexane, chloroform and ethyl acetate. Preliminary qualitative phytochemical screening was performed on the ethyl acetate fraction for capsule was revealed the presence of flavonoid and aromatic acids. These were examined by (high -performance liquid chromatography) (HPLC diodarray), (high- performance thin-layer chromatography)(HPTLC). Flavonoids were isolated by preparative layer chromatography and aromatic acid was isolated by preparative high-performance liquid chromatography HPLC from the ethyl acetate fraction of capsule. Then identified by High Performance Thin Layer Chromatography HPTLC, High performance liquid chromatography HPLC diode array , ultraviolet diode array UV-diode array and Liquid Chromatography /Mass Spectroscopy LC/MS. The chloroform fraction from the capsule was evaluated by Gas Chromatography//Mass Spectrometer(GC/MS). The different chromatographic and spectroscopic techniques revealed the presence of luteolin, apigenin and cinnamic acid in capsule of Cynara scolymus, also 9-octadecanoic acid (oleic acid), Oxalic acid, allyl tetradecyl ester, limonene, in chloroform of Cynara scolymus. The results of the current study proved the presence of luleolin, apigenin, and cinnamic acid in the ethyl acetate fraction of Cynara scolymus capsule. https://www.bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1076Cynara scolymus, Flavonoids, aromatic acid, ,High-performance liquid chromatography, High-performance thin-layer chromatography and G.C/MS |
| spellingShingle | Zaineb H. Ajeel Maha N. Hamad Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq Iraqi Journal of Pharmaceutical Sciences Cynara scolymus, Flavonoids, aromatic acid, ,High-performance liquid chromatography, High-performance thin-layer chromatography and G.C/MS |
| title | Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq |
| title_full | Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq |
| title_fullStr | Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq |
| title_full_unstemmed | Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq |
| title_short | Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq |
| title_sort | detection and isolation of flavonoid and aromatic acid from cynara scolymus different parts cultivated in iraq |
| topic | Cynara scolymus, Flavonoids, aromatic acid, ,High-performance liquid chromatography, High-performance thin-layer chromatography and G.C/MS |
| url | https://www.bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1076 |
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