Protective and Therapeutic Effect of Platelet-Rich Plasma on Experimental Cisplatin Ototoxicity
Objective:Cisplatin is a chemotherapeutic agent with an ototoxic effect that is frequently used in head and neck cancers. There are studies in the literature conducted with various antioxidant substances to protect and/or prevent ototoxicity. This study aims to investigate whether platelet-rich plas...
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| Format: | Article |
| Language: | English |
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Galenos Yayincilik
2023-06-01
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| Series: | Turkish Archives of Otorhinolaryngology |
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http://turkarchotolaryngol.net/archives/archive-detail/article-preview/protective-and-therapeutic-effect-of-platelet-rich/62114
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| _version_ | 1797682193553686528 |
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| author | Bahadır Gözaydın Asude Ünal Doğukan Özdemir Ayşe Çeçen Mustafa Bakırtaş |
| author_facet | Bahadır Gözaydın Asude Ünal Doğukan Özdemir Ayşe Çeçen Mustafa Bakırtaş |
| author_sort | Bahadır Gözaydın |
| collection | DOAJ |
| description | Objective:Cisplatin is a chemotherapeutic agent with an ototoxic effect that is frequently used in head and neck cancers. There are studies in the literature conducted with various antioxidant substances to protect and/or prevent ototoxicity. This study aims to investigate whether platelet-rich plasma (PRP) has a protective and therapeutic effect on cisplatin ototoxicity.Methods:A total of 40 Sprague Dawley albino rats were divided into six groups as control group (n=6), PRP-only group (n=6), cisplatin group (n=6), cisplatin + PRP group (n=6), PRP + cisplatin group (n=6), and donor group (n=10). At the beginning of the study and the 8th day, they were tested with distortion product otoacoustic emission (DPOAE). Assessment of DPOAE results was based on the signal-to-noise ratio in 2000, 3000, 4000, 6000, and 8000 Hz frequency bands. On the 8th day, the rats were sacrificed. For histological examinations, the temporal bones were dissected and fixed. After hematoxylin and eosin staining, the tissues were evaluated by light microscopy.Results:In the DPOAE tests performed on the 0th and 8th days of the cisplatin group, it was observed that cisplatin caused hearing loss in the rat ears. It was determined that the cisplatin group at 2000 Hz, 3000 Hz and 4000 Hz had a significant decrease in hearing compared to all groups (p<0.015), while the cisplatin group at 6000 and 8000 Hz was characterized by hearing loss at a higher rate than all groups (p<0.001). At the end of the study, negative effects of cisplatin on both cellular dimensions (cytoplasmic vacuolization, cell degeneration, dilatation, apoptotic cells, nerve degeneration) and hearing function were observed. No protective or therapeutic effect of PRP on cisplatin ototoxicity was observed.Conclusion:Our study showed that platelet-rich plasma did not have a significant effect in the treatment of hearing loss due to cisplatin ototoxicity and in preventing hearing loss in rats. |
| first_indexed | 2024-03-11T23:56:01Z |
| format | Article |
| id | doaj.art-68ca0faf733c4ff7836283ddb7ff3e12 |
| institution | Directory Open Access Journal |
| issn | 2667-7474 |
| language | English |
| last_indexed | 2024-03-11T23:56:01Z |
| publishDate | 2023-06-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Turkish Archives of Otorhinolaryngology |
| spelling | doaj.art-68ca0faf733c4ff7836283ddb7ff3e122023-09-18T12:46:13ZengGalenos YayincilikTurkish Archives of Otorhinolaryngology2667-74742023-06-01612758210.4274/tao.2023.2022-7-913049054Protective and Therapeutic Effect of Platelet-Rich Plasma on Experimental Cisplatin OtotoxicityBahadır Gözaydın0Asude Ünal1Doğukan Özdemir2Ayşe Çeçen3Mustafa Bakırtaş4 Clinic of Otorhinolaryngology, Ünye State Hospital, Ordu, Turkey Department of Otorhinolaryngology, University of Health Sciences, Samsun Training and Research Hospital, Samsun, Turkey Department of Otorhinolaryngology, Samsun University Faculty of Medicine, Samsun, Turkey Department of Otorhinolaryngology, Samsun University Faculty of Medicine, Samsun, Turkey Department of Pathology, University of Health Sciences, Samsun Training and Research Hospital, Samsun, Turkey Objective:Cisplatin is a chemotherapeutic agent with an ototoxic effect that is frequently used in head and neck cancers. There are studies in the literature conducted with various antioxidant substances to protect and/or prevent ototoxicity. This study aims to investigate whether platelet-rich plasma (PRP) has a protective and therapeutic effect on cisplatin ototoxicity.Methods:A total of 40 Sprague Dawley albino rats were divided into six groups as control group (n=6), PRP-only group (n=6), cisplatin group (n=6), cisplatin + PRP group (n=6), PRP + cisplatin group (n=6), and donor group (n=10). At the beginning of the study and the 8th day, they were tested with distortion product otoacoustic emission (DPOAE). Assessment of DPOAE results was based on the signal-to-noise ratio in 2000, 3000, 4000, 6000, and 8000 Hz frequency bands. On the 8th day, the rats were sacrificed. For histological examinations, the temporal bones were dissected and fixed. After hematoxylin and eosin staining, the tissues were evaluated by light microscopy.Results:In the DPOAE tests performed on the 0th and 8th days of the cisplatin group, it was observed that cisplatin caused hearing loss in the rat ears. It was determined that the cisplatin group at 2000 Hz, 3000 Hz and 4000 Hz had a significant decrease in hearing compared to all groups (p<0.015), while the cisplatin group at 6000 and 8000 Hz was characterized by hearing loss at a higher rate than all groups (p<0.001). At the end of the study, negative effects of cisplatin on both cellular dimensions (cytoplasmic vacuolization, cell degeneration, dilatation, apoptotic cells, nerve degeneration) and hearing function were observed. No protective or therapeutic effect of PRP on cisplatin ototoxicity was observed.Conclusion:Our study showed that platelet-rich plasma did not have a significant effect in the treatment of hearing loss due to cisplatin ototoxicity and in preventing hearing loss in rats. http://turkarchotolaryngol.net/archives/archive-detail/article-preview/protective-and-therapeutic-effect-of-platelet-rich/62114 ototoxicityhearing losscisplatinotoacoustic emissionsplatelet-rich plasmaanimal experimentation |
| spellingShingle | Bahadır Gözaydın Asude Ünal Doğukan Özdemir Ayşe Çeçen Mustafa Bakırtaş Protective and Therapeutic Effect of Platelet-Rich Plasma on Experimental Cisplatin Ototoxicity Turkish Archives of Otorhinolaryngology ototoxicity hearing loss cisplatin otoacoustic emissions platelet-rich plasma animal experimentation |
| title | Protective and Therapeutic Effect of Platelet-Rich Plasma on Experimental Cisplatin Ototoxicity |
| title_full | Protective and Therapeutic Effect of Platelet-Rich Plasma on Experimental Cisplatin Ototoxicity |
| title_fullStr | Protective and Therapeutic Effect of Platelet-Rich Plasma on Experimental Cisplatin Ototoxicity |
| title_full_unstemmed | Protective and Therapeutic Effect of Platelet-Rich Plasma on Experimental Cisplatin Ototoxicity |
| title_short | Protective and Therapeutic Effect of Platelet-Rich Plasma on Experimental Cisplatin Ototoxicity |
| title_sort | protective and therapeutic effect of platelet rich plasma on experimental cisplatin ototoxicity |
| topic | ototoxicity hearing loss cisplatin otoacoustic emissions platelet-rich plasma animal experimentation |
| url |
http://turkarchotolaryngol.net/archives/archive-detail/article-preview/protective-and-therapeutic-effect-of-platelet-rich/62114
|
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