Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose
Abstract Background Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial fermenters. However, the reduction of enzyme production caused by carbon ca...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | Biotechnology for Biofuels |
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| Online Access: | https://doi.org/10.1186/s13068-021-01887-0 |
| _version_ | 1818554561455980544 |
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| author | Nozomu Shibata Hiroshi Kakeshita Kazuaki Igarashi Yasushi Takimura Yosuke Shida Wataru Ogasawara Tohru Koda Tomohisa Hasunuma Akihiko Kondo |
| author_facet | Nozomu Shibata Hiroshi Kakeshita Kazuaki Igarashi Yasushi Takimura Yosuke Shida Wataru Ogasawara Tohru Koda Tomohisa Hasunuma Akihiko Kondo |
| author_sort | Nozomu Shibata |
| collection | DOAJ |
| description | Abstract Background Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial fermenters. However, the reduction of enzyme production caused by carbon catabolite repression (CCR) has long been a problem. Disruption of a typical transcriptional regulator, Cre1, does not sufficiently suppress this reduction in the presence of glucose. Results We found that deletion of an α-tubulin (tubB) in T. reesei enhanced both the amount and rate of secretory protein production. Also, the tubulin-disrupted (ΔtubB) strain had high enzyme production and the same enzyme profile even if the strain was cultured in a glucose-containing medium. From transcriptome analysis, the ΔtubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the ΔtubB strain. These results suggested that the ΔtubB strain was released from CCR. Conclusion Trichoderma reesei α-tubulin is involved in the transcription of cellulase and hemicellulase genes, as well as in CCR. This is the first report of overcoming CCR by disrupting α-tubulin gene in T. reesei. The disruption of α-tubulin is a promising approach for creating next-generation enzyme-producing strains of T. reesei. |
| first_indexed | 2024-12-12T09:41:26Z |
| format | Article |
| id | doaj.art-68cd2c8ac3524248819ef3b41e891306 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T09:41:26Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-68cd2c8ac3524248819ef3b41e8913062022-12-22T00:28:33ZengBMCBiotechnology for Biofuels1754-68342021-02-0114111610.1186/s13068-021-01887-0Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucoseNozomu Shibata0Hiroshi Kakeshita1Kazuaki Igarashi2Yasushi Takimura3Yosuke Shida4Wataru Ogasawara5Tohru Koda6Tomohisa Hasunuma7Akihiko Kondo8Biological Science Research, Kao CorporationBiological Science Research, Kao CorporationBiological Science Research, Kao CorporationBiological Science Research, Kao CorporationDepartment of Bioengineering, Nagaoka University of TechnologyDepartment of Bioengineering, Nagaoka University of TechnologyGraduate School of Science, Technology and Innovation, Kobe UniversityGraduate School of Science, Technology and Innovation, Kobe UniversityGraduate School of Science, Technology and Innovation, Kobe UniversityAbstract Background Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial fermenters. However, the reduction of enzyme production caused by carbon catabolite repression (CCR) has long been a problem. Disruption of a typical transcriptional regulator, Cre1, does not sufficiently suppress this reduction in the presence of glucose. Results We found that deletion of an α-tubulin (tubB) in T. reesei enhanced both the amount and rate of secretory protein production. Also, the tubulin-disrupted (ΔtubB) strain had high enzyme production and the same enzyme profile even if the strain was cultured in a glucose-containing medium. From transcriptome analysis, the ΔtubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the ΔtubB strain. These results suggested that the ΔtubB strain was released from CCR. Conclusion Trichoderma reesei α-tubulin is involved in the transcription of cellulase and hemicellulase genes, as well as in CCR. This is the first report of overcoming CCR by disrupting α-tubulin gene in T. reesei. The disruption of α-tubulin is a promising approach for creating next-generation enzyme-producing strains of T. reesei.https://doi.org/10.1186/s13068-021-01887-0Trichoderma reeseiBiomass saccharification enzymeCellulaseHemicellulaseCarbon catabolite repressionGlucose resistant |
| spellingShingle | Nozomu Shibata Hiroshi Kakeshita Kazuaki Igarashi Yasushi Takimura Yosuke Shida Wataru Ogasawara Tohru Koda Tomohisa Hasunuma Akihiko Kondo Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose Biotechnology for Biofuels Trichoderma reesei Biomass saccharification enzyme Cellulase Hemicellulase Carbon catabolite repression Glucose resistant |
| title | Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose |
| title_full | Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose |
| title_fullStr | Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose |
| title_full_unstemmed | Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose |
| title_short | Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose |
| title_sort | disruption of alpha tubulin releases carbon catabolite repression and enhances enzyme production in trichoderma reesei even in the presence of glucose |
| topic | Trichoderma reesei Biomass saccharification enzyme Cellulase Hemicellulase Carbon catabolite repression Glucose resistant |
| url | https://doi.org/10.1186/s13068-021-01887-0 |
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