Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty
Diabetic retinopathy (DR) is multifactorial by its origin, involving many cytokines and growth factors. Studies of cytokine levels in biological fluids seem to be relevant for an in-depth understanding of the disease pathogenesis. The purpose of the work was a comparative analysis of 45 cytokines at...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2020-04-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/1802 |
| _version_ | 1797197887708332032 |
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| author | V. V. Neroev O. V. Zaytseva N. V. Balatskaya A. A. Lazutova |
| author_facet | V. V. Neroev O. V. Zaytseva N. V. Balatskaya A. A. Lazutova |
| author_sort | V. V. Neroev |
| collection | DOAJ |
| description | Diabetic retinopathy (DR) is multifactorial by its origin, involving many cytokines and growth factors. Studies of cytokine levels in biological fluids seem to be relevant for an in-depth understanding of the disease pathogenesis. The purpose of the work was a comparative analysis of 45 cytokines at systemic (blood serum (BS)) and local (vitreous humor (VH)) levels in the patients with complicated proliferative DR, showing various features of the clinical pattern. The content of cytokines was tested in 53 samples of BS and 32 samples of VH in 53 patients with type 1 and type 2 diabetes mellitus with severe proliferative DR. We used the multiplex analysis technique by means of xMAP platform and Luminex xPONENT 3.1 program using 45-plex sets (Procarta Plex «eBioscience», Austria). 25 cytokines were detected at significant amounts in BS test samples, and 27 cytokines were revealed in VH specimens. Sensitivity limits of the test system allowed to find significantly higher levels of 7 cytokines (IL-6, IL-8, IP-10, MCP-1, HGF, LIF and VEGF-A) in VH samples, than in the BS, thus indicating to their local intraocular production. Correlations between the contents of VEGF-A growth factor and amounts of cytokines, including those involved in inflammatory reactions, are shown in VH, thus presuming the interrelation of pathogenetic components, i.e., inflammation and neoangiogenesis. The features of intraocular cytokine content were determined for various manifestations of diabetic ocular changes. Hemophthalmus has been shown to be associated with increased IL-8 and IP-10; iris rubeosis, with increase in LIF; proliferative DR activity was associated with higher MCP-1 levels, and extremely severe changes were related to increase in IL-6 and EGF. Testing of cytokines in biological fluids is informative when studying the mechanisms of inflammation, neoangiogenesis, and protective responses in pathogenesis of diabetic retinopathy. |
| first_indexed | 2024-03-08T05:48:45Z |
| format | Article |
| id | doaj.art-68ce7e7cddf7495e96315de301179999 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:51:06Z |
| publishDate | 2020-04-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-68ce7e7cddf7495e96315de3011799992024-04-22T13:07:44ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2020-04-0122230131010.15789/1563-0625-LAS-18021209Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopatyV. V. Neroev0O. V. Zaytseva1N. V. Balatskaya2A. A. Lazutova3Helmholtz National Medical Research Center of Eye DiseasesHelmholtz National Medical Research Center of Eye DiseasesHelmholtz National Medical Research Center of Eye DiseasesHelmholtz National Medical Research Center of Eye DiseasesDiabetic retinopathy (DR) is multifactorial by its origin, involving many cytokines and growth factors. Studies of cytokine levels in biological fluids seem to be relevant for an in-depth understanding of the disease pathogenesis. The purpose of the work was a comparative analysis of 45 cytokines at systemic (blood serum (BS)) and local (vitreous humor (VH)) levels in the patients with complicated proliferative DR, showing various features of the clinical pattern. The content of cytokines was tested in 53 samples of BS and 32 samples of VH in 53 patients with type 1 and type 2 diabetes mellitus with severe proliferative DR. We used the multiplex analysis technique by means of xMAP platform and Luminex xPONENT 3.1 program using 45-plex sets (Procarta Plex «eBioscience», Austria). 25 cytokines were detected at significant amounts in BS test samples, and 27 cytokines were revealed in VH specimens. Sensitivity limits of the test system allowed to find significantly higher levels of 7 cytokines (IL-6, IL-8, IP-10, MCP-1, HGF, LIF and VEGF-A) in VH samples, than in the BS, thus indicating to their local intraocular production. Correlations between the contents of VEGF-A growth factor and amounts of cytokines, including those involved in inflammatory reactions, are shown in VH, thus presuming the interrelation of pathogenetic components, i.e., inflammation and neoangiogenesis. The features of intraocular cytokine content were determined for various manifestations of diabetic ocular changes. Hemophthalmus has been shown to be associated with increased IL-8 and IP-10; iris rubeosis, with increase in LIF; proliferative DR activity was associated with higher MCP-1 levels, and extremely severe changes were related to increase in IL-6 and EGF. Testing of cytokines in biological fluids is informative when studying the mechanisms of inflammation, neoangiogenesis, and protective responses in pathogenesis of diabetic retinopathy.https://www.mimmun.ru/mimmun/article/view/1802diabetes mellitusdiabetic retinopathytractional retinal detachmenthemophthalmusiris rubeosispathogenesiscytokinesvitreous humorblood serum |
| spellingShingle | V. V. Neroev O. V. Zaytseva N. V. Balatskaya A. A. Lazutova Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty Медицинская иммунология diabetes mellitus diabetic retinopathy tractional retinal detachment hemophthalmus iris rubeosis pathogenesis cytokines vitreous humor blood serum |
| title | Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty |
| title_full | Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty |
| title_fullStr | Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty |
| title_full_unstemmed | Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty |
| title_short | Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty |
| title_sort | local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty |
| topic | diabetes mellitus diabetic retinopathy tractional retinal detachment hemophthalmus iris rubeosis pathogenesis cytokines vitreous humor blood serum |
| url | https://www.mimmun.ru/mimmun/article/view/1802 |
| work_keys_str_mv | AT vvneroev localandsystemicproductionof45cytokinesincomplicatedproliferativediabeticretinopaty AT ovzaytseva localandsystemicproductionof45cytokinesincomplicatedproliferativediabeticretinopaty AT nvbalatskaya localandsystemicproductionof45cytokinesincomplicatedproliferativediabeticretinopaty AT aalazutova localandsystemicproductionof45cytokinesincomplicatedproliferativediabeticretinopaty |