Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma
IntroductionOur previous studies have demonstrated that tumor-infiltrating lymphocytes (TILs), including normal B cells, T cells, and natural killer (NK) cells, in diffuse large B-cell lymphoma (DLBCL) have a significantly favorable impact on the clinical outcomes of patients treated with standard c...
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| Format: | Article |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1335689/full |
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| author | Tiantian Yu Zijun Y. Xu-Monette Zijun Y. Xu-Monette Anand Lagoo Wen Shuai Bangchen Wang Jadee Neff Luis F. Carrillo Eric D. Carlsen Eric D. Carlsen Sergio Pina-Oviedo Ken H. Young Ken H. Young |
| author_facet | Tiantian Yu Zijun Y. Xu-Monette Zijun Y. Xu-Monette Anand Lagoo Wen Shuai Bangchen Wang Jadee Neff Luis F. Carrillo Eric D. Carlsen Eric D. Carlsen Sergio Pina-Oviedo Ken H. Young Ken H. Young |
| author_sort | Tiantian Yu |
| collection | DOAJ |
| description | IntroductionOur previous studies have demonstrated that tumor-infiltrating lymphocytes (TILs), including normal B cells, T cells, and natural killer (NK) cells, in diffuse large B-cell lymphoma (DLBCL) have a significantly favorable impact on the clinical outcomes of patients treated with standard chemoimmunotherapy. In this study, to gain a full overview of the tumor immune microenvironment (TIME), we assembled a flow cytometry cohort of 102 patients diagnosed with DLBCL at the Duke University Medical Center.MethodsWe collected diagnostic flow cytometry data, including the proportion of T cells, abnormal B cells, normal B cells, plasma cells, NK cells, monocytes, and granulocytes in fresh biopsy tissues at clinical presentation, and analyzed the correlations with patient survival and between different cell populations.ResultsWe found that low T cell percentages in all viable cells and low ratios of T cells to abnormal B cells correlated with significantly poorer survival, whereas higher percentages of normal B cells among total B cells (or high ratios of normal B cells to abnormal B cells) and high percentages of NK cells among all viable cells correlated with significantly better survival in patients with DLBCL. After excluding a small number of patients with low T cell percentages, the normal B cell percentage among all B cells, but not T cell percentage among all cells, continued to show a remarkable prognostic effect. Data showed significant positive correlations between T cells and normal B cells, and between granulocytes and monocytes. Furthermore, we constructed a prognostic model based on clinical and flow cytometry factors, which divided the DLBCL cohort into two equal groups with remarkable differences in patient survival and treatment response.SummaryTILs, including normal B cells, T cells, and NK cells, are associated with favorable clinical outcomes in DLBCL, and flow cytometry capable of quantifying the TIME may have additional clinical utility for prognostication. |
| first_indexed | 2024-03-08T10:14:37Z |
| format | Article |
| id | doaj.art-68d6d3bcfc1a4a6291308b1158b27404 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-08T10:14:37Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-68d6d3bcfc1a4a6291308b1158b274042024-01-29T04:28:18ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-01-011510.3389/fimmu.2024.13356891335689Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphomaTiantian Yu0Zijun Y. Xu-Monette1Zijun Y. Xu-Monette2Anand Lagoo3Wen Shuai4Bangchen Wang5Jadee Neff6Luis F. Carrillo7Eric D. Carlsen8Eric D. Carlsen9Sergio Pina-Oviedo10Ken H. Young11Ken H. Young12Hematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesHematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesDuke University Cancer Institute, Durham, NC, United StatesHematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesHematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesDepartment of Pathology, Duke University Medical Center, Durham, NC, United StatesHematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesHematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesHematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesDuke University Cancer Institute, Durham, NC, United StatesHematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesHematopathology Division and Department of Pathology, Duke University Medical Center, Durham, NC, United StatesDuke University Cancer Institute, Durham, NC, United StatesIntroductionOur previous studies have demonstrated that tumor-infiltrating lymphocytes (TILs), including normal B cells, T cells, and natural killer (NK) cells, in diffuse large B-cell lymphoma (DLBCL) have a significantly favorable impact on the clinical outcomes of patients treated with standard chemoimmunotherapy. In this study, to gain a full overview of the tumor immune microenvironment (TIME), we assembled a flow cytometry cohort of 102 patients diagnosed with DLBCL at the Duke University Medical Center.MethodsWe collected diagnostic flow cytometry data, including the proportion of T cells, abnormal B cells, normal B cells, plasma cells, NK cells, monocytes, and granulocytes in fresh biopsy tissues at clinical presentation, and analyzed the correlations with patient survival and between different cell populations.ResultsWe found that low T cell percentages in all viable cells and low ratios of T cells to abnormal B cells correlated with significantly poorer survival, whereas higher percentages of normal B cells among total B cells (or high ratios of normal B cells to abnormal B cells) and high percentages of NK cells among all viable cells correlated with significantly better survival in patients with DLBCL. After excluding a small number of patients with low T cell percentages, the normal B cell percentage among all B cells, but not T cell percentage among all cells, continued to show a remarkable prognostic effect. Data showed significant positive correlations between T cells and normal B cells, and between granulocytes and monocytes. Furthermore, we constructed a prognostic model based on clinical and flow cytometry factors, which divided the DLBCL cohort into two equal groups with remarkable differences in patient survival and treatment response.SummaryTILs, including normal B cells, T cells, and NK cells, are associated with favorable clinical outcomes in DLBCL, and flow cytometry capable of quantifying the TIME may have additional clinical utility for prognostication.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1335689/fulltumor-infiltrating lymphocytesnormal B cellsTILDLBCLprognosismicroenvironment |
| spellingShingle | Tiantian Yu Zijun Y. Xu-Monette Zijun Y. Xu-Monette Anand Lagoo Wen Shuai Bangchen Wang Jadee Neff Luis F. Carrillo Eric D. Carlsen Eric D. Carlsen Sergio Pina-Oviedo Ken H. Young Ken H. Young Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma Frontiers in Immunology tumor-infiltrating lymphocytes normal B cells TIL DLBCL prognosis microenvironment |
| title | Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma |
| title_full | Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma |
| title_fullStr | Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma |
| title_full_unstemmed | Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma |
| title_short | Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma |
| title_sort | flow cytometry quantification of tumor infiltrating lymphocytes to predict the survival of patients with diffuse large b cell lymphoma |
| topic | tumor-infiltrating lymphocytes normal B cells TIL DLBCL prognosis microenvironment |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1335689/full |
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