Effect of new polyherbal formulations DF1911, DF2112 and DF2813 on CFA induced inflammation in rat model
Abstract Background Aim of the present study was to evaluate anti-inflammatory activity of newly developed polyherbal formulations DF1911, DF2112 and DF2813. These newly developed formulations are modifications of Dashamoola, a well known Ayurvedic formulation, along with addition of new plants. Met...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-04-01
|
| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1711-6 |
| _version_ | 1818049338022035456 |
|---|---|
| author | Bhagyashri Nagarkar Suresh Jagtap |
| author_facet | Bhagyashri Nagarkar Suresh Jagtap |
| author_sort | Bhagyashri Nagarkar |
| collection | DOAJ |
| description | Abstract Background Aim of the present study was to evaluate anti-inflammatory activity of newly developed polyherbal formulations DF1911, DF2112 and DF2813. These newly developed formulations are modifications of Dashamoola, a well known Ayurvedic formulation, along with addition of new plants. Methods Complete Freund’s adjuvant (CFA) induced inflammation in rat was used as an experimental model. Effects of the treatment in rats were monitored by physiological and biochemical parameters, histopathology and through gene expression studies. Results Diclofenac sodium showed maximum percentage inhibition (56.8 ± 3.5%) of paw edema followed by Dashamoola Kwatha (19.9 ± 1.8%). Among test formulations treated groups, DF1911 at 250 mg/kg bw (48.2 ± 5.4%, p < 0.001) and DF2112 at 250 mg/kg bw (49.9 ± 3.5%, p < 0.001) showed significant and maximum increase in percentage inhibition of paw edema as compared to Dashamoola Kwatha. Hematological alterations in CFA rats were normalized after treatment with test formulations. Results of serum markers and histopathological observations also supported the activity of formulations. Increased MDA levels in liver tissue of CFA injected animals significantly (p < 0.05) decreased by Diclofenac sodium and test formulation treated groups. DF1911, DF2112 and DF2813 showed down-regulation of IL1-β (~6.4-fold, ~5.2-fold and ~7.6-fold), IL-6 (~1.1-fold, ~1.6-fold and ~1.9-fold), TNF-α (~2.0-fold, ~4.6-fold and ~3.5-fold), and iNOS (~1.2-fold, ~1.8-fold and ~1.1-fold) in inflamed paw tissue compared to negative control group, respectively. Conclusions The anti-inflammatory effects of DF1911 and DF2112 in rats were significantly higher than the Dashamoola Kwatha and are comparable to Diclofenac sodium. |
| first_indexed | 2024-12-10T10:35:59Z |
| format | Article |
| id | doaj.art-68d8c81717a04cd8985b02bd1dbc0429 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-10T10:35:59Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-68d8c81717a04cd8985b02bd1dbc04292022-12-22T01:52:26ZengBMCBMC Complementary and Alternative Medicine1472-68822017-04-0117111210.1186/s12906-017-1711-6Effect of new polyherbal formulations DF1911, DF2112 and DF2813 on CFA induced inflammation in rat modelBhagyashri Nagarkar0Suresh Jagtap1Department of Herbal Medicine, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth Deemed UniversityDepartment of Herbal Medicine, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth Deemed UniversityAbstract Background Aim of the present study was to evaluate anti-inflammatory activity of newly developed polyherbal formulations DF1911, DF2112 and DF2813. These newly developed formulations are modifications of Dashamoola, a well known Ayurvedic formulation, along with addition of new plants. Methods Complete Freund’s adjuvant (CFA) induced inflammation in rat was used as an experimental model. Effects of the treatment in rats were monitored by physiological and biochemical parameters, histopathology and through gene expression studies. Results Diclofenac sodium showed maximum percentage inhibition (56.8 ± 3.5%) of paw edema followed by Dashamoola Kwatha (19.9 ± 1.8%). Among test formulations treated groups, DF1911 at 250 mg/kg bw (48.2 ± 5.4%, p < 0.001) and DF2112 at 250 mg/kg bw (49.9 ± 3.5%, p < 0.001) showed significant and maximum increase in percentage inhibition of paw edema as compared to Dashamoola Kwatha. Hematological alterations in CFA rats were normalized after treatment with test formulations. Results of serum markers and histopathological observations also supported the activity of formulations. Increased MDA levels in liver tissue of CFA injected animals significantly (p < 0.05) decreased by Diclofenac sodium and test formulation treated groups. DF1911, DF2112 and DF2813 showed down-regulation of IL1-β (~6.4-fold, ~5.2-fold and ~7.6-fold), IL-6 (~1.1-fold, ~1.6-fold and ~1.9-fold), TNF-α (~2.0-fold, ~4.6-fold and ~3.5-fold), and iNOS (~1.2-fold, ~1.8-fold and ~1.1-fold) in inflamed paw tissue compared to negative control group, respectively. Conclusions The anti-inflammatory effects of DF1911 and DF2112 in rats were significantly higher than the Dashamoola Kwatha and are comparable to Diclofenac sodium.http://link.springer.com/article/10.1186/s12906-017-1711-6Anti-inflammatoryNew poly-herbal formulationsDashamoola KwathaComplete Freund’s Adjuvant |
| spellingShingle | Bhagyashri Nagarkar Suresh Jagtap Effect of new polyherbal formulations DF1911, DF2112 and DF2813 on CFA induced inflammation in rat model BMC Complementary and Alternative Medicine Anti-inflammatory New poly-herbal formulations Dashamoola Kwatha Complete Freund’s Adjuvant |
| title | Effect of new polyherbal formulations DF1911, DF2112 and DF2813 on CFA induced inflammation in rat model |
| title_full | Effect of new polyherbal formulations DF1911, DF2112 and DF2813 on CFA induced inflammation in rat model |
| title_fullStr | Effect of new polyherbal formulations DF1911, DF2112 and DF2813 on CFA induced inflammation in rat model |
| title_full_unstemmed | Effect of new polyherbal formulations DF1911, DF2112 and DF2813 on CFA induced inflammation in rat model |
| title_short | Effect of new polyherbal formulations DF1911, DF2112 and DF2813 on CFA induced inflammation in rat model |
| title_sort | effect of new polyherbal formulations df1911 df2112 and df2813 on cfa induced inflammation in rat model |
| topic | Anti-inflammatory New poly-herbal formulations Dashamoola Kwatha Complete Freund’s Adjuvant |
| url | http://link.springer.com/article/10.1186/s12906-017-1711-6 |
| work_keys_str_mv | AT bhagyashrinagarkar effectofnewpolyherbalformulationsdf1911df2112anddf2813oncfainducedinflammationinratmodel AT sureshjagtap effectofnewpolyherbalformulationsdf1911df2112anddf2813oncfainducedinflammationinratmodel |