Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.)
In the present investigation it was studied the effect of oxidative stress on root of red beet. The degree of stress exposure was assessed at various levels: in tissues of roots (conductometric method), on isolated vacuoles (time-lapse video recording), and transport activity of the vacuolar membran...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
"Vikol publishing" ST Kolesnichenko V.V.
2014-12-01
|
| Series: | Journal of Stress Physiology & Biochemistry |
| Subjects: | |
| Online Access: | http://www.jspb.ru/issues/2014/N4/JSPB_2014_4_05-12.pdf |
| _version_ | 1818834687177523200 |
|---|---|
| author | E.V. Kolesnikova N.V. Ozolina V.N. Nurminsky I.S. Nesterkina L.A. Sitneva T.I. Lapteva |
| author_facet | E.V. Kolesnikova N.V. Ozolina V.N. Nurminsky I.S. Nesterkina L.A. Sitneva T.I. Lapteva |
| author_sort | E.V. Kolesnikova |
| collection | DOAJ |
| description | In the present investigation it was studied the effect of oxidative stress on root of red beet. The degree of stress exposure was assessed at various levels: in tissues of roots (conductometric method), on isolated vacuoles (time-lapse video recording), and transport activity of the vacuolar membrane enzyme, namely V-H+-ATPase (fluorescent probe method). The obtained results allow to conclude the negative effects of oxidative stress on different levels of plant organization. There were several effects: a significant increase of outflow of electrolytes from the tissues of roots, a great decrease of half-life time of isolated vacuoles, and a 2 times reduction of transport activity of tonoplast H+-ATPase. Thus, the methods used in this study can be applied to assess the intensity of the impact of oxidative stress on plants. |
| first_indexed | 2024-12-19T02:38:46Z |
| format | Article |
| id | doaj.art-68d9466348ce4df5be2e62fc41ee460d |
| institution | Directory Open Access Journal |
| issn | 1997-0838 |
| language | English |
| last_indexed | 2024-12-19T02:38:46Z |
| publishDate | 2014-12-01 |
| publisher | "Vikol publishing" ST Kolesnichenko V.V. |
| record_format | Article |
| series | Journal of Stress Physiology & Biochemistry |
| spelling | doaj.art-68d9466348ce4df5be2e62fc41ee460d2022-12-21T20:39:13Zeng"Vikol publishing" ST Kolesnichenko V.V.Journal of Stress Physiology & Biochemistry1997-08382014-12-01104512Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.)E.V. Kolesnikova0N.V. Ozolina1V.N. Nurminsky2I.S. Nesterkina3L.A. Sitneva4T.I. Lapteva5Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk, 664033, RussiaSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk, 664033, RussiaSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk, 664033, RussiaSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk, 664033, RussiaSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk, 664033, RussiaSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, PO 317, Irkutsk, 664033, RussiaIn the present investigation it was studied the effect of oxidative stress on root of red beet. The degree of stress exposure was assessed at various levels: in tissues of roots (conductometric method), on isolated vacuoles (time-lapse video recording), and transport activity of the vacuolar membrane enzyme, namely V-H+-ATPase (fluorescent probe method). The obtained results allow to conclude the negative effects of oxidative stress on different levels of plant organization. There were several effects: a significant increase of outflow of electrolytes from the tissues of roots, a great decrease of half-life time of isolated vacuoles, and a 2 times reduction of transport activity of tonoplast H+-ATPase. Thus, the methods used in this study can be applied to assess the intensity of the impact of oxidative stress on plants.http://www.jspb.ru/issues/2014/N4/JSPB_2014_4_05-12.pdfenzyme activityelectrolyte outflowroot of red beetoxidative stressstability of vacuoles |
| spellingShingle | E.V. Kolesnikova N.V. Ozolina V.N. Nurminsky I.S. Nesterkina L.A. Sitneva T.I. Lapteva Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.) Journal of Stress Physiology & Biochemistry enzyme activity electrolyte outflow root of red beet oxidative stress stability of vacuoles |
| title | Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.) |
| title_full | Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.) |
| title_fullStr | Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.) |
| title_full_unstemmed | Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.) |
| title_short | Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.) |
| title_sort | evaluation of the effect of oxidative stress on roots of red beet beta vulgaris l |
| topic | enzyme activity electrolyte outflow root of red beet oxidative stress stability of vacuoles |
| url | http://www.jspb.ru/issues/2014/N4/JSPB_2014_4_05-12.pdf |
| work_keys_str_mv | AT evkolesnikova evaluationoftheeffectofoxidativestressonrootsofredbeetbetavulgarisl AT nvozolina evaluationoftheeffectofoxidativestressonrootsofredbeetbetavulgarisl AT vnnurminsky evaluationoftheeffectofoxidativestressonrootsofredbeetbetavulgarisl AT isnesterkina evaluationoftheeffectofoxidativestressonrootsofredbeetbetavulgarisl AT lasitneva evaluationoftheeffectofoxidativestressonrootsofredbeetbetavulgarisl AT tilapteva evaluationoftheeffectofoxidativestressonrootsofredbeetbetavulgarisl |