The Effects of Anti-Vascular Endothelial Growth Factor Drugs on Retinal Pigment Epithelial Cell Culture
Objectives:To assess the effects of anti-vascular endothelial growth factor (VEGF) drugs on retinal pigment epithelium cell culture.Materials and Methods:Aflibercept (0.5 mg/mL), bevacizumab (0.3125 mg/mL), and ranibizumab (0.125 mg/mL) were applied to retinal pigment epithelium cell cultures isolat...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2018-08-01
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| Series: | Türk Oftalmoloji Dergisi |
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http://www.oftalmoloji.org/archives/archive-detail/article-preview/the-effects-of-anti-vascular-endothelial-growth-fa/19565
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| _version_ | 1797913557177729024 |
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| author | Mustafa Şahiner Dilek Bahar Ayşe Öner Zeynep Burçin Gönen Metin Ünlü Duygu Gülmez Sevim Çağatay Karaca Galip Ertuğrul Mirza |
| author_facet | Mustafa Şahiner Dilek Bahar Ayşe Öner Zeynep Burçin Gönen Metin Ünlü Duygu Gülmez Sevim Çağatay Karaca Galip Ertuğrul Mirza |
| author_sort | Mustafa Şahiner |
| collection | DOAJ |
| description | Objectives:To assess the effects of anti-vascular endothelial growth factor (VEGF) drugs on retinal pigment epithelium cell culture.Materials and Methods:Aflibercept (0.5 mg/mL), bevacizumab (0.3125 mg/mL), and ranibizumab (0.125 mg/mL) were applied to retinal pigment epithelium cell cultures isolated from the enucleated eyes of New Zealand white rabbits. Viability, apoptosis, proliferation, and senescence of the cells were evaluated in control and drug-treated cultures at the end of 72 hours.Results:Cells treated with aflibercept showed increased viability and decreased apoptosis compared to the control culture and both the bevacizumab- and ranibizumab-treated groups (p<0.05). Statistically increased apoptosis and decreased viability were found in the bevacizumab and ranibizumab-treated groups compared with the control group (p<0.05). There were no statistically significant differences in cell proliferation and senescence between the groups (p>0.05).Conclusion:Anti-VEGF drugs did not affect senescence or proliferation of retinal pigment epithelium cells. Aflibercept was found to decrease apoptosis and increase cell viability, while ranibizumab and bevacizumab increased apoptosis and reduced cell viability in retinal pigment epithelium culture. |
| first_indexed | 2024-04-10T12:13:31Z |
| format | Article |
| id | doaj.art-68ddede8c4d44b9fbdcc9ca74364744e |
| institution | Directory Open Access Journal |
| issn | 1300-0659 2147-2661 |
| language | English |
| last_indexed | 2024-04-10T12:13:31Z |
| publishDate | 2018-08-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Türk Oftalmoloji Dergisi |
| spelling | doaj.art-68ddede8c4d44b9fbdcc9ca74364744e2023-02-15T16:15:53ZengGalenos YayineviTürk Oftalmoloji Dergisi1300-06592147-26612018-08-0148419019510.4274/tjo.2027013049054The Effects of Anti-Vascular Endothelial Growth Factor Drugs on Retinal Pigment Epithelial Cell CultureMustafa Şahiner0Dilek Bahar1Ayşe Öner2Zeynep Burçin Gönen3Metin Ünlü4Duygu Gülmez Sevim5Çağatay Karaca6Galip Ertuğrul Mirza7 Erciyes Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Kayseri, Türkiye Erciyes Üniversitesi Betül-Ziya Eren Genom ve Kök Hücre Merkezi, Kayseri, Türkiye Erciyes Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Kayseri, Türkiye Erciyes Üniversitesi Diş Hekimliği Fakültesi, Oral/Maksillofasyal Cerrahi Anabilim Dalı, Kayseri, Türkiye Erciyes Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Kayseri, Türkiye Erciyes Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Kayseri, Türkiye Erciyes Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Kayseri, Türkiye Erciyes Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Kayseri, Türkiye Objectives:To assess the effects of anti-vascular endothelial growth factor (VEGF) drugs on retinal pigment epithelium cell culture.Materials and Methods:Aflibercept (0.5 mg/mL), bevacizumab (0.3125 mg/mL), and ranibizumab (0.125 mg/mL) were applied to retinal pigment epithelium cell cultures isolated from the enucleated eyes of New Zealand white rabbits. Viability, apoptosis, proliferation, and senescence of the cells were evaluated in control and drug-treated cultures at the end of 72 hours.Results:Cells treated with aflibercept showed increased viability and decreased apoptosis compared to the control culture and both the bevacizumab- and ranibizumab-treated groups (p<0.05). Statistically increased apoptosis and decreased viability were found in the bevacizumab and ranibizumab-treated groups compared with the control group (p<0.05). There were no statistically significant differences in cell proliferation and senescence between the groups (p>0.05).Conclusion:Anti-VEGF drugs did not affect senescence or proliferation of retinal pigment epithelium cells. Aflibercept was found to decrease apoptosis and increase cell viability, while ranibizumab and bevacizumab increased apoptosis and reduced cell viability in retinal pigment epithelium culture. http://www.oftalmoloji.org/archives/archive-detail/article-preview/the-effects-of-anti-vascular-endothelial-growth-fa/19565 Anti-VEGFcell culturesenescenceretinal pigment epithelial cell |
| spellingShingle | Mustafa Şahiner Dilek Bahar Ayşe Öner Zeynep Burçin Gönen Metin Ünlü Duygu Gülmez Sevim Çağatay Karaca Galip Ertuğrul Mirza The Effects of Anti-Vascular Endothelial Growth Factor Drugs on Retinal Pigment Epithelial Cell Culture Türk Oftalmoloji Dergisi Anti-VEGF cell culture senescence retinal pigment epithelial cell |
| title | The Effects of Anti-Vascular Endothelial Growth Factor Drugs on Retinal Pigment Epithelial Cell Culture |
| title_full | The Effects of Anti-Vascular Endothelial Growth Factor Drugs on Retinal Pigment Epithelial Cell Culture |
| title_fullStr | The Effects of Anti-Vascular Endothelial Growth Factor Drugs on Retinal Pigment Epithelial Cell Culture |
| title_full_unstemmed | The Effects of Anti-Vascular Endothelial Growth Factor Drugs on Retinal Pigment Epithelial Cell Culture |
| title_short | The Effects of Anti-Vascular Endothelial Growth Factor Drugs on Retinal Pigment Epithelial Cell Culture |
| title_sort | effects of anti vascular endothelial growth factor drugs on retinal pigment epithelial cell culture |
| topic | Anti-VEGF cell culture senescence retinal pigment epithelial cell |
| url |
http://www.oftalmoloji.org/archives/archive-detail/article-preview/the-effects-of-anti-vascular-endothelial-growth-fa/19565
|
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