THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA
This study aimed to determine the role of guava fruit filtrate (GFF) on maintaining the pre-freezing and post-thawed quality of Bali bull spermatozoa in tris-egg yolk (TEY) extender. The bovine semen was collected using artificial vagina twice a week (n= 10). Ejaculate samples were divided into four...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Indonesian |
| Published: |
Syiah Kuala University
2018-10-01
|
| Series: | Jurnal Kedokteran Hewan |
| Subjects: | |
| Online Access: | https://jurnal.usk.ac.id/JKH/article/view/8801 |
| _version_ | 1797278986406985728 |
|---|---|
| author | I Wayan Lanus Sumadiasa Lukman Lukman Rodiah Rodiah |
| author_facet | I Wayan Lanus Sumadiasa Lukman Lukman Rodiah Rodiah |
| author_sort | I Wayan Lanus Sumadiasa |
| collection | DOAJ |
| description | This study aimed to determine the role of guava fruit filtrate (GFF) on maintaining the pre-freezing and post-thawed quality of Bali bull
spermatozoa in tris-egg yolk (TEY) extender. The bovine semen was collected using artificial vagina twice a week (n= 10). Ejaculate samples
were divided into four tubes and each tube was respectively added the following diluents: TEY, TEY + 5% GFF, TEY + 10% GFF, and TEY +
15% GFF. The diluted semen was filled into the straws (0.25 mL) and cooled in refrigerator at 4° C for 2.5 hours. The samples were kept on
vapor phase of liquid nitrogen (N2) (-120° C) for 10 minutes prior to be stored in liquid phase (-196° C). The quality of pre-freezing and postthawed
spermatozoa in straw sample (i.e. motility, viability, and abnormality) was evaluated under light microscopy at 400x magnification. Data
were analysed statistically. The percentage of motility and viability both on pre-freezing and post-thawed process were significantly higher in
semen diluted with TEY + 10% GFF compared to control (TEY), TEY + 5% GFF, and TEY + 15% GFF. Addition of 10% GFF into tris-egg yolk
extender play role for maintaining the quality of pre-freezing and post-thawed Bali bull spermatozoa. |
| first_indexed | 2024-03-07T16:16:54Z |
| format | Article |
| id | doaj.art-68df75bb1e824a60b78a1ca048ffacee |
| institution | Directory Open Access Journal |
| issn | 1978-225X 2502-5600 |
| language | Indonesian |
| last_indexed | 2024-03-07T16:16:54Z |
| publishDate | 2018-10-01 |
| publisher | Syiah Kuala University |
| record_format | Article |
| series | Jurnal Kedokteran Hewan |
| spelling | doaj.art-68df75bb1e824a60b78a1ca048ffacee2024-03-04T10:29:00ZindSyiah Kuala UniversityJurnal Kedokteran Hewan1978-225X2502-56002018-10-0112210.21157/j.ked.hewan.v12i2.88018891THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOAI Wayan Lanus Sumadiasa0Lukman Lukman1Rodiah Rodiah2Animal Reproduction Laboratory, Faculty of Animal Husbandry, Mataram University, Nusa Tenggara Barat, IndonesiaAnimal Reproduction Laboratory, Faculty of Animal Husbandry, Mataram University, Nusa Tenggara Barat, IndonesiaAnimal Reproduction Laboratory, Faculty of Animal Husbandry, Mataram University, Nusa Tenggara Barat, IndonesiaThis study aimed to determine the role of guava fruit filtrate (GFF) on maintaining the pre-freezing and post-thawed quality of Bali bull spermatozoa in tris-egg yolk (TEY) extender. The bovine semen was collected using artificial vagina twice a week (n= 10). Ejaculate samples were divided into four tubes and each tube was respectively added the following diluents: TEY, TEY + 5% GFF, TEY + 10% GFF, and TEY + 15% GFF. The diluted semen was filled into the straws (0.25 mL) and cooled in refrigerator at 4° C for 2.5 hours. The samples were kept on vapor phase of liquid nitrogen (N2) (-120° C) for 10 minutes prior to be stored in liquid phase (-196° C). The quality of pre-freezing and postthawed spermatozoa in straw sample (i.e. motility, viability, and abnormality) was evaluated under light microscopy at 400x magnification. Data were analysed statistically. The percentage of motility and viability both on pre-freezing and post-thawed process were significantly higher in semen diluted with TEY + 10% GFF compared to control (TEY), TEY + 5% GFF, and TEY + 15% GFF. Addition of 10% GFF into tris-egg yolk extender play role for maintaining the quality of pre-freezing and post-thawed Bali bull spermatozoa.https://jurnal.usk.ac.id/JKH/article/view/8801bovineguava filtratepre-freezing and post-thawedspermatozoa |
| spellingShingle | I Wayan Lanus Sumadiasa Lukman Lukman Rodiah Rodiah THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA Jurnal Kedokteran Hewan bovine guava filtrate pre-freezing and post-thawed spermatozoa |
| title | THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA |
| title_full | THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA |
| title_fullStr | THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA |
| title_full_unstemmed | THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA |
| title_short | THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA |
| title_sort | role of guava fruit filtrate on maintaining the pre freezing and post thawed quality of bali bull spermatozoa |
| topic | bovine guava filtrate pre-freezing and post-thawed spermatozoa |
| url | https://jurnal.usk.ac.id/JKH/article/view/8801 |
| work_keys_str_mv | AT iwayanlanussumadiasa theroleofguavafruitfiltrateonmaintainingtheprefreezingandpostthawedqualityofbalibullspermatozoa AT lukmanlukman theroleofguavafruitfiltrateonmaintainingtheprefreezingandpostthawedqualityofbalibullspermatozoa AT rodiahrodiah theroleofguavafruitfiltrateonmaintainingtheprefreezingandpostthawedqualityofbalibullspermatozoa AT iwayanlanussumadiasa roleofguavafruitfiltrateonmaintainingtheprefreezingandpostthawedqualityofbalibullspermatozoa AT lukmanlukman roleofguavafruitfiltrateonmaintainingtheprefreezingandpostthawedqualityofbalibullspermatozoa AT rodiahrodiah roleofguavafruitfiltrateonmaintainingtheprefreezingandpostthawedqualityofbalibullspermatozoa |