Reduced Sodium Portions Favor Osteogenic Properties and Cytocompatibility of 45S5-Based Bioactive Glass Particles
Besides its favorable biological properties, the release of sodium (Na) from the well-known 45S5-bioactive glass (BG) composition (in mol%: 46.1, SiO<sub>2</sub>, 24.5 CaO, 24.5 Na<sub>2</sub>O, 6.0 P<sub>2</sub>O<sub>5</sub>) can hamper its cytocompat...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/2313-7673/8/6/472 |
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| author | Stefanos Tsitlakidis Frederike Hohenbild Merve Saur Arash Moghaddam Elke Kunisch Tobias Renkawitz Isabel Gonzalo de Juan Fabian Westhauser |
| author_facet | Stefanos Tsitlakidis Frederike Hohenbild Merve Saur Arash Moghaddam Elke Kunisch Tobias Renkawitz Isabel Gonzalo de Juan Fabian Westhauser |
| author_sort | Stefanos Tsitlakidis |
| collection | DOAJ |
| description | Besides its favorable biological properties, the release of sodium (Na) from the well-known 45S5-bioactive glass (BG) composition (in mol%: 46.1, SiO<sub>2</sub>, 24.5 CaO, 24.5 Na<sub>2</sub>O, 6.0 P<sub>2</sub>O<sub>5</sub>) can hamper its cytocompatibility. In this study, particles of Na-reduced variants of 45S5-BG were produced in exchange for CaO and P<sub>2</sub>O<sub>5</sub> via the sol-gel-route resulting in Na contents of 75%, 50%, 25% or 0% of the original composition. The release of ions from the BGs as well as their impact on the cell environment (pH values), viability and osteogenic differentiation (activity of alkaline phosphatase (ALP)), the expression of osteopontin and osteocalcin in human bone-marrow-derived mesenchymal stromal cells in correlation to the Na-content and ion release of the BGs was assessed. The release of Na-ions increased with increasing Na-content in the BGs. With decreasing Na content, the viability of cells incubated with the BGs increased. The Na-reduced BGs showed elevated ALP activity and a pro-osteogenic stimulation with accelerated osteopontin induction and a pronounced upregulation of osteocalcin. In conclusion, the reduction in Na-content enhances the cytocompatibility and improves the osteogenic properties of 45S5-BG, making the Na-reduced variants of 45S5-BG promising candidates for further experimental consideration. |
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| language | English |
| last_indexed | 2024-03-10T21:24:30Z |
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| spelling | doaj.art-68e237d7b7a744528322de47044d7f4b2023-11-19T15:48:38ZengMDPI AGBiomimetics2313-76732023-10-018647210.3390/biomimetics8060472Reduced Sodium Portions Favor Osteogenic Properties and Cytocompatibility of 45S5-Based Bioactive Glass ParticlesStefanos Tsitlakidis0Frederike Hohenbild1Merve Saur2Arash Moghaddam3Elke Kunisch4Tobias Renkawitz5Isabel Gonzalo de Juan6Fabian Westhauser7Department of Orthopaedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, GermanyDepartment of Orthopaedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, GermanyDepartment of Orthopaedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, GermanyPrivatÄrztliches Zentrum Aschaffenburg, Frohsinnstraße 12, 63739 Aschaffenburg, GermanyDepartment of Orthopaedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, GermanyDepartment of Orthopaedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, GermanyInstitut für Materialwissenschaft, Technische Universität Darmstadt, Otto-Berndt-Straße 3, 64287 Darmstadt, GermanyDepartment of Orthopaedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, GermanyBesides its favorable biological properties, the release of sodium (Na) from the well-known 45S5-bioactive glass (BG) composition (in mol%: 46.1, SiO<sub>2</sub>, 24.5 CaO, 24.5 Na<sub>2</sub>O, 6.0 P<sub>2</sub>O<sub>5</sub>) can hamper its cytocompatibility. In this study, particles of Na-reduced variants of 45S5-BG were produced in exchange for CaO and P<sub>2</sub>O<sub>5</sub> via the sol-gel-route resulting in Na contents of 75%, 50%, 25% or 0% of the original composition. The release of ions from the BGs as well as their impact on the cell environment (pH values), viability and osteogenic differentiation (activity of alkaline phosphatase (ALP)), the expression of osteopontin and osteocalcin in human bone-marrow-derived mesenchymal stromal cells in correlation to the Na-content and ion release of the BGs was assessed. The release of Na-ions increased with increasing Na-content in the BGs. With decreasing Na content, the viability of cells incubated with the BGs increased. The Na-reduced BGs showed elevated ALP activity and a pro-osteogenic stimulation with accelerated osteopontin induction and a pronounced upregulation of osteocalcin. In conclusion, the reduction in Na-content enhances the cytocompatibility and improves the osteogenic properties of 45S5-BG, making the Na-reduced variants of 45S5-BG promising candidates for further experimental consideration.https://www.mdpi.com/2313-7673/8/6/472sodium-reduced bioactive glass45S5 bioactive glasssol gelbone tissue engineeringhuman mesenchymal stromal cellsosteogenic differentiation |
| spellingShingle | Stefanos Tsitlakidis Frederike Hohenbild Merve Saur Arash Moghaddam Elke Kunisch Tobias Renkawitz Isabel Gonzalo de Juan Fabian Westhauser Reduced Sodium Portions Favor Osteogenic Properties and Cytocompatibility of 45S5-Based Bioactive Glass Particles Biomimetics sodium-reduced bioactive glass 45S5 bioactive glass sol gel bone tissue engineering human mesenchymal stromal cells osteogenic differentiation |
| title | Reduced Sodium Portions Favor Osteogenic Properties and Cytocompatibility of 45S5-Based Bioactive Glass Particles |
| title_full | Reduced Sodium Portions Favor Osteogenic Properties and Cytocompatibility of 45S5-Based Bioactive Glass Particles |
| title_fullStr | Reduced Sodium Portions Favor Osteogenic Properties and Cytocompatibility of 45S5-Based Bioactive Glass Particles |
| title_full_unstemmed | Reduced Sodium Portions Favor Osteogenic Properties and Cytocompatibility of 45S5-Based Bioactive Glass Particles |
| title_short | Reduced Sodium Portions Favor Osteogenic Properties and Cytocompatibility of 45S5-Based Bioactive Glass Particles |
| title_sort | reduced sodium portions favor osteogenic properties and cytocompatibility of 45s5 based bioactive glass particles |
| topic | sodium-reduced bioactive glass 45S5 bioactive glass sol gel bone tissue engineering human mesenchymal stromal cells osteogenic differentiation |
| url | https://www.mdpi.com/2313-7673/8/6/472 |
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