Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsy
Abstract Exosomes are extracellular vesicles that actively secreted by most live cells via endosome pathway. They distribute ubiquitously in body fluids and play important roles in physiological and pathological processes. Recent studies have revealed that exosomes carry cell‐specific cargoes, such...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-04-01
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| Online Access: | https://doi.org/10.1002/VIW.20220048 |
| _version_ | 1827960702703239168 |
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| author | Yuqing Wang Shurong Wang Lanting Li Yan Zou Baohong Liu Xiaoni Fang |
| author_facet | Yuqing Wang Shurong Wang Lanting Li Yan Zou Baohong Liu Xiaoni Fang |
| author_sort | Yuqing Wang |
| collection | DOAJ |
| description | Abstract Exosomes are extracellular vesicles that actively secreted by most live cells via endosome pathway. They distribute ubiquitously in body fluids and play important roles in physiological and pathological processes. Recent studies have revealed that exosomes carry cell‐specific cargoes, such as proteins and nucleic acids, which have been recognized as emerging noninvasive biomarkers in liquid biopsy. Therefore, the molecular profiling of tumor‐derived exosomes is crucial in facilitating the discovery of cancer‐related biomarkers. Recently, various techniques have been proposed for sensitive, accurate, and rapid molecular characterization of these small vesicles. To meet the growing demands for multiplexed detection, microfluidics has been integrated with diverse traditional analytical methods as powerful components for exosomes analysis. Taking advantages of their inherent merits of great sensitivity, high analysis performance and system integration, short analysis time, low cost, small volume of sample, improved potential for control, automation, and disposability, microfluidic systems have significantly improved the molecular profiling of exosomes. In this review, we summarized the most recent progresses in microfluidic technologies for molecular profiling of tumor‐derived exosomes, including proteins, nucleic acids, metabolites, lipids. Furthermore, we discussed the major challenges and offered a perspective of the microfluidic strategy‐based exosomal biomarkers discovery. We expected that the in‐depth summary of microfluidic devices–assisted analysis of exosomes will greatly facilitate the discovery of biomarkers, thereby realizing highly efficient and accurate diagnosis, and prediction of cancers. Moreover, in view of the significance of this interesting approach in exosomes‐based liquid biopsy, this review will contribute to the translation of microfluidic technology into clinical applications. |
| first_indexed | 2024-04-09T16:13:23Z |
| format | Article |
| id | doaj.art-68e3bdd7cdb042a681419f21a79dbe8b |
| institution | Directory Open Access Journal |
| issn | 2688-3988 2688-268X |
| language | English |
| last_indexed | 2024-04-09T16:13:23Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | View |
| spelling | doaj.art-68e3bdd7cdb042a681419f21a79dbe8b2023-04-24T07:56:34ZengWileyView2688-39882688-268X2023-04-0142n/an/a10.1002/VIW.20220048Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsyYuqing Wang0Shurong Wang1Lanting Li2Yan Zou3Baohong Liu4Xiaoni Fang5School of Pharmacy Shanghai Stomatological Hospital Department of Chemistry Fudan University Shanghai ChinaSchool of Pharmacy Shanghai Stomatological Hospital Department of Chemistry Fudan University Shanghai ChinaSchool of Pharmacy Shanghai Stomatological Hospital Department of Chemistry Fudan University Shanghai ChinaSchool of Pharmacy Shanghai Stomatological Hospital Department of Chemistry Fudan University Shanghai ChinaSchool of Pharmacy Shanghai Stomatological Hospital Department of Chemistry Fudan University Shanghai ChinaSchool of Pharmacy Shanghai Stomatological Hospital Department of Chemistry Fudan University Shanghai ChinaAbstract Exosomes are extracellular vesicles that actively secreted by most live cells via endosome pathway. They distribute ubiquitously in body fluids and play important roles in physiological and pathological processes. Recent studies have revealed that exosomes carry cell‐specific cargoes, such as proteins and nucleic acids, which have been recognized as emerging noninvasive biomarkers in liquid biopsy. Therefore, the molecular profiling of tumor‐derived exosomes is crucial in facilitating the discovery of cancer‐related biomarkers. Recently, various techniques have been proposed for sensitive, accurate, and rapid molecular characterization of these small vesicles. To meet the growing demands for multiplexed detection, microfluidics has been integrated with diverse traditional analytical methods as powerful components for exosomes analysis. Taking advantages of their inherent merits of great sensitivity, high analysis performance and system integration, short analysis time, low cost, small volume of sample, improved potential for control, automation, and disposability, microfluidic systems have significantly improved the molecular profiling of exosomes. In this review, we summarized the most recent progresses in microfluidic technologies for molecular profiling of tumor‐derived exosomes, including proteins, nucleic acids, metabolites, lipids. Furthermore, we discussed the major challenges and offered a perspective of the microfluidic strategy‐based exosomal biomarkers discovery. We expected that the in‐depth summary of microfluidic devices–assisted analysis of exosomes will greatly facilitate the discovery of biomarkers, thereby realizing highly efficient and accurate diagnosis, and prediction of cancers. Moreover, in view of the significance of this interesting approach in exosomes‐based liquid biopsy, this review will contribute to the translation of microfluidic technology into clinical applications.https://doi.org/10.1002/VIW.20220048biomarkerexosomesliquid biopsymicrofluidicsmolecular profiling |
| spellingShingle | Yuqing Wang Shurong Wang Lanting Li Yan Zou Baohong Liu Xiaoni Fang Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsy View biomarker exosomes liquid biopsy microfluidics molecular profiling |
| title | Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsy |
| title_full | Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsy |
| title_fullStr | Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsy |
| title_full_unstemmed | Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsy |
| title_short | Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsy |
| title_sort | microfluidics based molecular profiling of tumor derived exosomes for liquid biopsy |
| topic | biomarker exosomes liquid biopsy microfluidics molecular profiling |
| url | https://doi.org/10.1002/VIW.20220048 |
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