CytoBuoy: a step forward towards using flow cytometry in operational oceanography
While the performance of biological sensors in real time monitoring networks is limited to bulk values like chlorophyll fluorescence, in practice the implementation of automated phytoplankton taxonomy remains a remote option. Aiming to reduce this gap we developed a flow cytometer called CytoBuoy fo...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Consejo Superior de Investigaciones Científicas
2000-06-01
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| Series: | Scientia Marina |
| Subjects: | |
| Online Access: | http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/759 |
| _version_ | 1818667588790517760 |
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| author | G. B.J. Dubelaar P. L. Gerritzen |
| author_facet | G. B.J. Dubelaar P. L. Gerritzen |
| author_sort | G. B.J. Dubelaar |
| collection | DOAJ |
| description | While the performance of biological sensors in real time monitoring networks is limited to bulk values like chlorophyll fluorescence, in practice the implementation of automated phytoplankton taxonomy remains a remote option. Aiming to reduce this gap we developed a flow cytometer called CytoBuoy for autonomous in situ operation, for instance in a moored buoy with wireless data transfer. Although not comparable to microscopy, flow cytometers detect and count particles allowing a limited level of particle characterization based on the light scatter and fluorescence properties of the individual particles. CytoBuoy analyses a large size range of particles, typical for marine coastal zones and fresh waters. The `field´ design implies a tradeoff between the accuracy and versatility of laboratory flow cytometers and the qualities needed for trouble free autonomous operation in situ. The optics and electronics however were designed for maximal reflection of the particle morphology in the measured signals. Whereas standard cytometers reduce these to single peak or area `listmode´ numbers, the signal courses are preserved fully by CytoBuoy and transferred to the computer as raw data, which allows more extended morphological analysis. Extended field tests will have to show how the system holds in various environments and weather conditions. |
| first_indexed | 2024-12-17T06:22:49Z |
| format | Article |
| id | doaj.art-68e4ef48d2f547859473bcace5b77019 |
| institution | Directory Open Access Journal |
| issn | 0214-8358 1886-8134 |
| language | English |
| last_indexed | 2024-12-17T06:22:49Z |
| publishDate | 2000-06-01 |
| publisher | Consejo Superior de Investigaciones Científicas |
| record_format | Article |
| series | Scientia Marina |
| spelling | doaj.art-68e4ef48d2f547859473bcace5b770192022-12-21T22:00:21ZengConsejo Superior de Investigaciones CientíficasScientia Marina0214-83581886-81342000-06-0164225526510.3989/scimar.2000.64n2255751CytoBuoy: a step forward towards using flow cytometry in operational oceanographyG. B.J. Dubelaar0P. L. Gerritzen1Dubelaar Research Instruments EngineeringDatawell b.v. Laboratory for InstrumentationWhile the performance of biological sensors in real time monitoring networks is limited to bulk values like chlorophyll fluorescence, in practice the implementation of automated phytoplankton taxonomy remains a remote option. Aiming to reduce this gap we developed a flow cytometer called CytoBuoy for autonomous in situ operation, for instance in a moored buoy with wireless data transfer. Although not comparable to microscopy, flow cytometers detect and count particles allowing a limited level of particle characterization based on the light scatter and fluorescence properties of the individual particles. CytoBuoy analyses a large size range of particles, typical for marine coastal zones and fresh waters. The `field´ design implies a tradeoff between the accuracy and versatility of laboratory flow cytometers and the qualities needed for trouble free autonomous operation in situ. The optics and electronics however were designed for maximal reflection of the particle morphology in the measured signals. Whereas standard cytometers reduce these to single peak or area `listmode´ numbers, the signal courses are preserved fully by CytoBuoy and transferred to the computer as raw data, which allows more extended morphological analysis. Extended field tests will have to show how the system holds in various environments and weather conditions.http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/759cytobuoyin situ flow cytometryphytoplankton analysismarine monitoring |
| spellingShingle | G. B.J. Dubelaar P. L. Gerritzen CytoBuoy: a step forward towards using flow cytometry in operational oceanography Scientia Marina cytobuoy in situ flow cytometry phytoplankton analysis marine monitoring |
| title | CytoBuoy: a step forward towards using flow cytometry in operational oceanography |
| title_full | CytoBuoy: a step forward towards using flow cytometry in operational oceanography |
| title_fullStr | CytoBuoy: a step forward towards using flow cytometry in operational oceanography |
| title_full_unstemmed | CytoBuoy: a step forward towards using flow cytometry in operational oceanography |
| title_short | CytoBuoy: a step forward towards using flow cytometry in operational oceanography |
| title_sort | cytobuoy a step forward towards using flow cytometry in operational oceanography |
| topic | cytobuoy in situ flow cytometry phytoplankton analysis marine monitoring |
| url | http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/759 |
| work_keys_str_mv | AT gbjdubelaar cytobuoyastepforwardtowardsusingflowcytometryinoperationaloceanography AT plgerritzen cytobuoyastepforwardtowardsusingflowcytometryinoperationaloceanography |