Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expression
In vivo-collected granulosa cells (GC) from the southern white rhinoceros (SWR) provide a non-invasive assessment of the developmental status of oocytes prior to in vitro culture, which could aid in the development of assisted reproductive technologies (ART). Our study aimed to investigate gene expr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Theriogenology Wild |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773093X23000399 |
| _version_ | 1797615857414701056 |
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| author | Elena Ruggeri Kristin Klohonatz Marc-André Sirard Barbara Durrant Stephen Coleman |
| author_facet | Elena Ruggeri Kristin Klohonatz Marc-André Sirard Barbara Durrant Stephen Coleman |
| author_sort | Elena Ruggeri |
| collection | DOAJ |
| description | In vivo-collected granulosa cells (GC) from the southern white rhinoceros (SWR) provide a non-invasive assessment of the developmental status of oocytes prior to in vitro culture, which could aid in the development of assisted reproductive technologies (ART). Our study aimed to investigate gene expression in SWR granulosa cells, collected in vivo and gain preliminary insight into the transcriptional activity occurring within the cells during various stages of oocyte development. It was hypothesized there would be similarities between the SWR GC transcriptome and cattle and humans, two species for which well-annotated genomes are available and ART are commonly used. GC were collected from SWR following ovum pickup (OPU) and pooled from all aspirated follicles. Total RNA was isolated, libraries prepared, and sequencing performed using an Illumina NextSeq 500. Reads were aligned and annotated to CerSimCot1.0. Databases for cattle and human were acquired for comparison. This study identified 37,407 transcripts present in GC of SWR. It was determined that cattle and human transcriptomes are valuable resources with a homology of 45 % with the SWR. In conclusion, these data provide preliminary, novel insights into the transcriptional activity of GC in the SWR that can be used to enhance ART in this species. |
| first_indexed | 2024-03-11T07:32:55Z |
| format | Article |
| id | doaj.art-68e5c5e02bcc4e5c9c204ba67abcc9bb |
| institution | Directory Open Access Journal |
| issn | 2773-093X |
| language | English |
| last_indexed | 2024-03-11T07:32:55Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Theriogenology Wild |
| spelling | doaj.art-68e5c5e02bcc4e5c9c204ba67abcc9bb2023-11-17T05:28:47ZengElsevierTheriogenology Wild2773-093X2023-01-013100055Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expressionElena Ruggeri0Kristin Klohonatz1Marc-André Sirard2Barbara Durrant3Stephen Coleman4Reproductive Sciences, Conservation Science Wildlife Health, San Diego Zoo Wildlife Alliance, 15600 San Pasqual Valley Road, Escondido, CA 92027, United States of America; Corresponding author.Select Breeders Services, 961 Cayots Corner Road, Chesapeake City, MD 21915, United States of AmericaDépartement des Sciences Animales, Pavillon des services, Bureau 2732, Université Laval, Québec City, Québec, Canada G1V0A6Reproductive Sciences, Conservation Science Wildlife Health, San Diego Zoo Wildlife Alliance, 15600 San Pasqual Valley Road, Escondido, CA 92027, United States of AmericaDepartment of Animal Sciences, ANSC 110, Colorado State University, 1171 Campus Delivery, Fort Collins, CO 80523, United States of AmericaIn vivo-collected granulosa cells (GC) from the southern white rhinoceros (SWR) provide a non-invasive assessment of the developmental status of oocytes prior to in vitro culture, which could aid in the development of assisted reproductive technologies (ART). Our study aimed to investigate gene expression in SWR granulosa cells, collected in vivo and gain preliminary insight into the transcriptional activity occurring within the cells during various stages of oocyte development. It was hypothesized there would be similarities between the SWR GC transcriptome and cattle and humans, two species for which well-annotated genomes are available and ART are commonly used. GC were collected from SWR following ovum pickup (OPU) and pooled from all aspirated follicles. Total RNA was isolated, libraries prepared, and sequencing performed using an Illumina NextSeq 500. Reads were aligned and annotated to CerSimCot1.0. Databases for cattle and human were acquired for comparison. This study identified 37,407 transcripts present in GC of SWR. It was determined that cattle and human transcriptomes are valuable resources with a homology of 45 % with the SWR. In conclusion, these data provide preliminary, novel insights into the transcriptional activity of GC in the SWR that can be used to enhance ART in this species.http://www.sciencedirect.com/science/article/pii/S2773093X23000399Granulosa cellsRhinocerosAssisted reproductive technologiesTranscriptomeGene expression |
| spellingShingle | Elena Ruggeri Kristin Klohonatz Marc-André Sirard Barbara Durrant Stephen Coleman Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expression Theriogenology Wild Granulosa cells Rhinoceros Assisted reproductive technologies Transcriptome Gene expression |
| title | Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expression |
| title_full | Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expression |
| title_fullStr | Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expression |
| title_full_unstemmed | Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expression |
| title_short | Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expression |
| title_sort | genomic insights into southern white rhinoceros ceratotherium simum simum reproduction revealing granulosa cell gene expression |
| topic | Granulosa cells Rhinoceros Assisted reproductive technologies Transcriptome Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2773093X23000399 |
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