Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi
The larches, the Larix genus of plants are known as a natural source of taxifolin (dihydroquercetin), and extracts of its taxifolin rich xylem are used in dietary supplements to maintain health. In the present study, to assess biological activities of a methanol extract of the Japanese larch, Larix...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584401935707X |
| _version_ | 1819168733682204672 |
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| author | Daisuke Muramatsu Hirofumi Uchiyama Hiroshi Kida Atsushi Iwai |
| author_facet | Daisuke Muramatsu Hirofumi Uchiyama Hiroshi Kida Atsushi Iwai |
| author_sort | Daisuke Muramatsu |
| collection | DOAJ |
| description | The larches, the Larix genus of plants are known as a natural source of taxifolin (dihydroquercetin), and extracts of its taxifolin rich xylem are used in dietary supplements to maintain health. In the present study, to assess biological activities of a methanol extract of the Japanese larch, Larix kaempferi (LK-ME), the effects of LK-ME on cell viability, inflammatory cytokine expression, and glycation were investigated. The effects of taxifolin which is known to be a main compound of LK-ME, and its related flavonoids, quercetin and luteolin were also examined. The results show that taxifolin exhibits lower growth inhibition activity and lesser induction activity of inflammatory cytokines in a human monocyte derived cell line, THP-1 cells, while in vitro anti-glycation activities of taxifolin were inhibiting at comparable levels to those of quercetin and luteolin. The growth inhibition and the cytokine induction activities, and the anti-glycation effects of LK-ME are assumed to have properties similar to taxifolin. The results of high performance liquid chromatography (HPLC) analysis indicated that taxifolin was detected as the main peak of LK-ME at the absorbance of 280 nm, and the concentration of taxifolin was measured as 3.12 mg/ml. The actual concentration of taxifolin in LK-ME is lower than the concentration estimated from the IC50 values calculated by the results of glycation assays, suggesting that other compounds contained in LK-ME are involved in the anti-glycation activity. |
| first_indexed | 2024-12-22T19:08:18Z |
| format | Article |
| id | doaj.art-68ec324337c94863b71359ccfddd0c53 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-22T19:08:18Z |
| publishDate | 2019-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-68ec324337c94863b71359ccfddd0c532022-12-21T18:15:44ZengElsevierHeliyon2405-84402019-07-0157e02047Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferiDaisuke Muramatsu0Hirofumi Uchiyama1Hiroshi Kida2Atsushi Iwai3Aureo Science Co., Ltd., Hokudai Business Spring, North 21, West 12, Kita-ku, Sapporo, Hokkaido, 001-0021, Japan; Aureo Co., Ltd., 54-1 Kazusakoito, Kimitsu, Chiba, 292-1149, JapanAureo Science Co., Ltd., Hokudai Business Spring, North 21, West 12, Kita-ku, Sapporo, Hokkaido, 001-0021, Japan; Aureo Co., Ltd., 54-1 Kazusakoito, Kimitsu, Chiba, 292-1149, JapanHokkaido University Research Center for Zoonosis Control, North 20, West 10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanAureo Science Co., Ltd., Hokudai Business Spring, North 21, West 12, Kita-ku, Sapporo, Hokkaido, 001-0021, Japan; Aureo Co., Ltd., 54-1 Kazusakoito, Kimitsu, Chiba, 292-1149, Japan; Corresponding author.The larches, the Larix genus of plants are known as a natural source of taxifolin (dihydroquercetin), and extracts of its taxifolin rich xylem are used in dietary supplements to maintain health. In the present study, to assess biological activities of a methanol extract of the Japanese larch, Larix kaempferi (LK-ME), the effects of LK-ME on cell viability, inflammatory cytokine expression, and glycation were investigated. The effects of taxifolin which is known to be a main compound of LK-ME, and its related flavonoids, quercetin and luteolin were also examined. The results show that taxifolin exhibits lower growth inhibition activity and lesser induction activity of inflammatory cytokines in a human monocyte derived cell line, THP-1 cells, while in vitro anti-glycation activities of taxifolin were inhibiting at comparable levels to those of quercetin and luteolin. The growth inhibition and the cytokine induction activities, and the anti-glycation effects of LK-ME are assumed to have properties similar to taxifolin. The results of high performance liquid chromatography (HPLC) analysis indicated that taxifolin was detected as the main peak of LK-ME at the absorbance of 280 nm, and the concentration of taxifolin was measured as 3.12 mg/ml. The actual concentration of taxifolin in LK-ME is lower than the concentration estimated from the IC50 values calculated by the results of glycation assays, suggesting that other compounds contained in LK-ME are involved in the anti-glycation activity.http://www.sciencedirect.com/science/article/pii/S240584401935707XFood scienceFunctional foodsProtein glycationImmune responseGlycationLarix kaempferi |
| spellingShingle | Daisuke Muramatsu Hirofumi Uchiyama Hiroshi Kida Atsushi Iwai Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi Heliyon Food science Functional foods Protein glycation Immune response Glycation Larix kaempferi |
| title | Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi |
| title_full | Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi |
| title_fullStr | Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi |
| title_full_unstemmed | Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi |
| title_short | Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi |
| title_sort | cell cytotoxity and anti glycation activity of taxifolin rich extract from japanese larch larix kaempferi |
| topic | Food science Functional foods Protein glycation Immune response Glycation Larix kaempferi |
| url | http://www.sciencedirect.com/science/article/pii/S240584401935707X |
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