CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DE Lactococcus lactis SOBRE Yersinia pseudotuberculosis
Lactic acid bacteria have demonstrated a high ability to inhibit pathogenic microorganisms, which improve knowledge of such microorganisms is important, for this, the kinetics was determined, growth and the inhibition effect of Lactococcus lactis on Yersinia pseudotuberculosis. The research was cond...
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| Format: | Article |
| Language: | Spanish |
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Universidad del Cauca
2016-12-01
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| Series: | Biotecnología en el Sector Agropecuario y Agroindustrial |
| Subjects: | |
| Online Access: | http://revistabiotecnologia.unicauca.edu.co/revista/index.php/biotecnologia/article/view/1651/374 |
| _version_ | 1828492383286722560 |
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| author | HENRY JURADO GÁMEZ ALEJANDRO ROMERO BENAVIDES ALEXANDER MORILLO GARCES |
| author_facet | HENRY JURADO GÁMEZ ALEJANDRO ROMERO BENAVIDES ALEXANDER MORILLO GARCES |
| author_sort | HENRY JURADO GÁMEZ |
| collection | DOAJ |
| description | Lactic acid bacteria have demonstrated a high ability to inhibit pathogenic microorganisms, which improve knowledge of such microorganisms is important, for this, the kinetics was determined, growth and the inhibition effect of Lactococcus lactis on Yersinia pseudotuberculosis. The research was conducted at the University of Nariño, by susceptibility testing in all strains; in vitro inhibition of Lc. lactis and supernatant on bacterial pathogen; gastrointestinal lactic strain testing (gas production and catalase, bile, bile salts and 2 temperatures), growth kinetics and HPLC determination of peptides in the supernatant. dicloxacillin resistance was found in both strains. Lactic strain and the supernatant inhibited Y. pseudotuberculosis. growths and 3,9x1010 3x1011 CFU/150 uL to 3 to 5% bile salts, 3x1011 5x1012 and CFU/150 uL 1 and 2% bovine 3x1013 and 3x1012 bile and CFU/150 uL was found 38 and 45°C. The logarithmic phase of Lc. lactis was found at 3 hours with values 6,4x1012 CFU/150 uL. The VAL-TIR-VAL peptide was found in the supernatant. It is concluded that Lc lactis shows probiotic characteristics in in vitro conditions. |
| first_indexed | 2024-12-11T11:19:25Z |
| format | Article |
| id | doaj.art-68eeb99cb4d848ebb0548041a7391114 |
| institution | Directory Open Access Journal |
| issn | 1692-3561 1909-9959 |
| language | Spanish |
| last_indexed | 2024-12-11T11:19:25Z |
| publishDate | 2016-12-01 |
| publisher | Universidad del Cauca |
| record_format | Article |
| series | Biotecnología en el Sector Agropecuario y Agroindustrial |
| spelling | doaj.art-68eeb99cb4d848ebb0548041a73911142022-12-22T01:09:13ZspaUniversidad del CaucaBiotecnología en el Sector Agropecuario y Agroindustrial1692-35611909-99592016-12-01142182810.18684/BSAA(14)18-28S1692-35612016000200003CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DE Lactococcus lactis SOBRE Yersinia pseudotuberculosisHENRY JURADO GÁMEZ0ALEJANDRO ROMERO BENAVIDES1ALEXANDER MORILLO GARCES Universidad de Nariño, Facultad de Ciencias Pecuarias, Departamento de Producción y Procesamiento Animal, Programa de Zootecnia, Grupo de investigación FISE-PROBIOTEC. Ph. D. Ingeniería de alimentos. Pasto, Colombia.Universidad de Nariño, Grupo de investigación FISE-PROBIOTEC. Zootecnista. Pasto, Colombia.Lactic acid bacteria have demonstrated a high ability to inhibit pathogenic microorganisms, which improve knowledge of such microorganisms is important, for this, the kinetics was determined, growth and the inhibition effect of Lactococcus lactis on Yersinia pseudotuberculosis. The research was conducted at the University of Nariño, by susceptibility testing in all strains; in vitro inhibition of Lc. lactis and supernatant on bacterial pathogen; gastrointestinal lactic strain testing (gas production and catalase, bile, bile salts and 2 temperatures), growth kinetics and HPLC determination of peptides in the supernatant. dicloxacillin resistance was found in both strains. Lactic strain and the supernatant inhibited Y. pseudotuberculosis. growths and 3,9x1010 3x1011 CFU/150 uL to 3 to 5% bile salts, 3x1011 5x1012 and CFU/150 uL 1 and 2% bovine 3x1013 and 3x1012 bile and CFU/150 uL was found 38 and 45°C. The logarithmic phase of Lc. lactis was found at 3 hours with values 6,4x1012 CFU/150 uL. The VAL-TIR-VAL peptide was found in the supernatant. It is concluded that Lc lactis shows probiotic characteristics in in vitro conditions.http://revistabiotecnologia.unicauca.edu.co/revista/index.php/biotecnologia/article/view/1651/374ProbioticsAntibioticsFermentationAntagonism. |
| spellingShingle | HENRY JURADO GÁMEZ ALEJANDRO ROMERO BENAVIDES ALEXANDER MORILLO GARCES CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DE Lactococcus lactis SOBRE Yersinia pseudotuberculosis Biotecnología en el Sector Agropecuario y Agroindustrial Probiotics Antibiotics Fermentation Antagonism. |
| title | CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DE Lactococcus lactis SOBRE Yersinia pseudotuberculosis |
| title_full | CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DE Lactococcus lactis SOBRE Yersinia pseudotuberculosis |
| title_fullStr | CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DE Lactococcus lactis SOBRE Yersinia pseudotuberculosis |
| title_full_unstemmed | CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DE Lactococcus lactis SOBRE Yersinia pseudotuberculosis |
| title_short | CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DE Lactococcus lactis SOBRE Yersinia pseudotuberculosis |
| title_sort | cinetica prueba de crecimiento y efecto de inhibicion de lactococcus lactis sobre yersinia pseudotuberculosis |
| topic | Probiotics Antibiotics Fermentation Antagonism. |
| url | http://revistabiotecnologia.unicauca.edu.co/revista/index.php/biotecnologia/article/view/1651/374 |
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