Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay
The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/9/3/109 |
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| author | Sobhan Sepehri Björn Agnarsson Teresa Zardán Gómez de la Torre Justin F. Schneiderman Jakob Blomgren Aldo Jesorka Christer Johansson Mats Nilsson Jan Albert Maria Strømme Dag Winkler Alexei Kalaboukhov |
| author_facet | Sobhan Sepehri Björn Agnarsson Teresa Zardán Gómez de la Torre Justin F. Schneiderman Jakob Blomgren Aldo Jesorka Christer Johansson Mats Nilsson Jan Albert Maria Strømme Dag Winkler Alexei Kalaboukhov |
| author_sort | Sobhan Sepehri |
| collection | DOAJ |
| description | The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes. |
| first_indexed | 2024-12-22T13:18:00Z |
| format | Article |
| id | doaj.art-68f1e315335e49c0a7dce31e3edc583b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-22T13:18:00Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-68f1e315335e49c0a7dce31e3edc583b2022-12-21T18:24:32ZengMDPI AGBiosensors2079-63742019-09-019310910.3390/bios9030109bios9030109Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic AssaySobhan Sepehri0Björn Agnarsson1Teresa Zardán Gómez de la Torre2Justin F. Schneiderman3Jakob Blomgren4Aldo Jesorka5Christer Johansson6Mats Nilsson7Jan Albert8Maria Strømme9Dag Winkler10Alexei Kalaboukhov11Department of Microtechnology and Nanoscience—MC2, Chalmers University of Technology, SE-412 96 Göteborg, SwedenDepartment of Physics, Chalmers University of Technology, SE-412 96 Göteborg, SwedenDepartment of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, SwedenDepartment of Microtechnology and Nanoscience—MC2, Chalmers University of Technology, SE-412 96 Göteborg, SwedenRISE—Research Institutes of Sweden, SE-411 33 Göteborg, SwedenDepartment of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Göteborg, SwedenRISE—Research Institutes of Sweden, SE-411 33 Göteborg, SwedenScience for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Box 1031, SE-171 21 Solna, SwedenDepartment of Clinical Microbiology, Karolinska University Hospital, SE-171 76 Stockholm, SwedenDepartment of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, SwedenDepartment of Microtechnology and Nanoscience—MC2, Chalmers University of Technology, SE-412 96 Göteborg, SwedenDepartment of Microtechnology and Nanoscience—MC2, Chalmers University of Technology, SE-412 96 Göteborg, SwedenThe specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes.https://www.mdpi.com/2079-6374/9/3/109magnetic nanoparticlebioassaydifferential homogenous magnetic assayimmobilizationbinding kineticsrolling circle amplification product |
| spellingShingle | Sobhan Sepehri Björn Agnarsson Teresa Zardán Gómez de la Torre Justin F. Schneiderman Jakob Blomgren Aldo Jesorka Christer Johansson Mats Nilsson Jan Albert Maria Strømme Dag Winkler Alexei Kalaboukhov Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay Biosensors magnetic nanoparticle bioassay differential homogenous magnetic assay immobilization binding kinetics rolling circle amplification product |
| title | Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay |
| title_full | Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay |
| title_fullStr | Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay |
| title_full_unstemmed | Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay |
| title_short | Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay |
| title_sort | characterization of binding of magnetic nanoparticles to rolling circle amplification products by turn on magnetic assay |
| topic | magnetic nanoparticle bioassay differential homogenous magnetic assay immobilization binding kinetics rolling circle amplification product |
| url | https://www.mdpi.com/2079-6374/9/3/109 |
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