Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism
Despite the scientific evidence supporting their biostimulant activity, the molecular mechanism(s) underlying the activity of protein hydrolysates (PHs) and the specificity among different products are still poorly explored. This work tested five different protein hydrolysates, produced from differe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/2/326 |
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| author | Angela Valentina Ceccarelli Begoña Miras-Moreno Valentina Buffagni Biancamaria Senizza Youry Pii Mariateresa Cardarelli Youssef Rouphael Giuseppe Colla Luigi Lucini |
| author_facet | Angela Valentina Ceccarelli Begoña Miras-Moreno Valentina Buffagni Biancamaria Senizza Youry Pii Mariateresa Cardarelli Youssef Rouphael Giuseppe Colla Luigi Lucini |
| author_sort | Angela Valentina Ceccarelli |
| collection | DOAJ |
| description | Despite the scientific evidence supporting their biostimulant activity, the molecular mechanism(s) underlying the activity of protein hydrolysates (PHs) and the specificity among different products are still poorly explored. This work tested five different protein hydrolysates, produced from different plant sources using the same enzymatic approach, for their ability to promote rooting in tomato cuttings following quick dipping. Provided that all the different PHs increased root length (45–93%) and some of them increased root number (37–56%), untargeted metabolomics followed by multivariate statistics and pathway analysis were used to unravel the molecular processes at the basis of the biostimulant activity. Distinct metabolomic signatures could be found in roots following the PHs treatments. In general, PHs shaped the phytohormone profile, modulating the complex interaction between cytokinins and auxins, an interplay playing a pivotal role in root development, and triggered a down accumulation of brassinosteroids. Concerning secondary metabolism, PHs induced the accumulation of aliphatic glucosinolates, alkaloids, and phenylpropanoids, potentially eliciting crop resilience to stress conditions. Here, we confirm that PHs may have a hormone-like activity, and that their application can modulate plant growth, likely interfering with signaling processes. Noteworthy, the heterogenicity of the botanical origin supported the distinctive and peculiar metabolomic responses we observed across the products tested. While supporting their biostimulant activity, these findings suggest that a generalized crop response to PHs cannot be defined and that specific effects are rather to be investigated. |
| first_indexed | 2024-03-09T05:05:59Z |
| format | Article |
| id | doaj.art-68f78cca1f8640fca1a2974b05bb960b |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T05:05:59Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-68f78cca1f8640fca1a2974b05bb960b2023-12-03T12:54:47ZengMDPI AGPlants2223-77472021-02-0110232610.3390/plants10020326Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and MetabolismAngela Valentina Ceccarelli0Begoña Miras-Moreno1Valentina Buffagni2Biancamaria Senizza3Youry Pii4Mariateresa Cardarelli5Youssef Rouphael6Giuseppe Colla7Luigi Lucini8Department of Agriculture and Forest Sciences, University of Tuscia, 01100 Viterbo, ItalyDepartment for Sustainable Food Process, Research Centre for Nutrigenomics and Proteomics, Università Cattolica del Sacro Cuore, 29122 Piacenza, ItalyDepartment for Sustainable Food Process, Research Centre for Nutrigenomics and Proteomics, Università Cattolica del Sacro Cuore, 29122 Piacenza, ItalyDepartment for Sustainable Food Process, Research Centre for Nutrigenomics and Proteomics, Università Cattolica del Sacro Cuore, 29122 Piacenza, ItalyFaculty of Science and Technology, Free University of Bozen, 39100 Bolzano, ItalyConsiglio per la Ricerca in Agricoltura e L’analisi Dell’economia Agraria, Centro di Ricerca Orticoltura e Florovivaismo, 84098 Pontecagnano Faiano, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, 80055 Portici, ItalyDepartment of Agriculture and Forest Sciences, University of Tuscia, 01100 Viterbo, ItalyDepartment for Sustainable Food Process, Research Centre for Nutrigenomics and Proteomics, Università Cattolica del Sacro Cuore, 29122 Piacenza, ItalyDespite the scientific evidence supporting their biostimulant activity, the molecular mechanism(s) underlying the activity of protein hydrolysates (PHs) and the specificity among different products are still poorly explored. This work tested five different protein hydrolysates, produced from different plant sources using the same enzymatic approach, for their ability to promote rooting in tomato cuttings following quick dipping. Provided that all the different PHs increased root length (45–93%) and some of them increased root number (37–56%), untargeted metabolomics followed by multivariate statistics and pathway analysis were used to unravel the molecular processes at the basis of the biostimulant activity. Distinct metabolomic signatures could be found in roots following the PHs treatments. In general, PHs shaped the phytohormone profile, modulating the complex interaction between cytokinins and auxins, an interplay playing a pivotal role in root development, and triggered a down accumulation of brassinosteroids. Concerning secondary metabolism, PHs induced the accumulation of aliphatic glucosinolates, alkaloids, and phenylpropanoids, potentially eliciting crop resilience to stress conditions. Here, we confirm that PHs may have a hormone-like activity, and that their application can modulate plant growth, likely interfering with signaling processes. Noteworthy, the heterogenicity of the botanical origin supported the distinctive and peculiar metabolomic responses we observed across the products tested. While supporting their biostimulant activity, these findings suggest that a generalized crop response to PHs cannot be defined and that specific effects are rather to be investigated.https://www.mdpi.com/2223-7747/10/2/326biostimulantsmetabolomicshormone-like activityplant bioassay<i>Solanum lycoperscum</i> L. |
| spellingShingle | Angela Valentina Ceccarelli Begoña Miras-Moreno Valentina Buffagni Biancamaria Senizza Youry Pii Mariateresa Cardarelli Youssef Rouphael Giuseppe Colla Luigi Lucini Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism Plants biostimulants metabolomics hormone-like activity plant bioassay <i>Solanum lycoperscum</i> L. |
| title | Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism |
| title_full | Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism |
| title_fullStr | Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism |
| title_full_unstemmed | Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism |
| title_short | Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism |
| title_sort | foliar application of different vegetal derived protein hydrolysates distinctively modulates tomato root development and metabolism |
| topic | biostimulants metabolomics hormone-like activity plant bioassay <i>Solanum lycoperscum</i> L. |
| url | https://www.mdpi.com/2223-7747/10/2/326 |
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