MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice
Physiological and pathophysiological differences widely exist in paired organ systems. However, the molecular basis for these differences remains largely unknown. We previously reported that there exist differentially expressed miRNAs (DEMs) in the left and right kidneys of normal mice. Here, we ide...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Molecular Therapy: Nucleic Acids |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253120301955 |
| _version_ | 1818559011013787648 |
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| author | Jiangcheng Shi Chengqing Hu Yuan Zhou Chunmei Cui Jichun Yang Qinghua Cui |
| author_facet | Jiangcheng Shi Chengqing Hu Yuan Zhou Chunmei Cui Jichun Yang Qinghua Cui |
| author_sort | Jiangcheng Shi |
| collection | DOAJ |
| description | Physiological and pathophysiological differences widely exist in paired organ systems. However, the molecular basis for these differences remains largely unknown. We previously reported that there exist differentially expressed miRNAs (DEMs) in the left and right kidneys of normal mice. Here, we identified the DEMs in the left and right eyes, lungs, and testes of normal mice via RNA sequencing. As a result, we identified 26 DEMs in eyes, with 23 higher and 3 lower in the left eyes compared with right eyes; 21 DEMs in lungs, with 15 higher and 6 lower in the left lungs compared with right lungs; and 54 DEMs in testes, with 6 higher and 48 lower in the left testes compared with right testes. Ten microRNAs (miRNAs) were further examined by quantitative PCR assays, and seven of these were confirmed. In addition, correlation analysis was performed between paired organ miRNA expressions and diverse body fluid miRNA expressions. Finally, we explored the functions and networks of DEMs and performed biological process and pathway enrichment analysis of target genes for DEMs, providing insights into the physiological and pathophysiological differences between the two entities of paired organs. |
| first_indexed | 2024-12-14T00:19:47Z |
| format | Article |
| id | doaj.art-68fbcdd3ec6449a4bf782d0549f4a0a2 |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-14T00:19:47Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-68fbcdd3ec6449a4bf782d0549f4a0a22022-12-21T23:25:17ZengElsevierMolecular Therapy: Nucleic Acids2162-25312020-09-0121687695MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal MiceJiangcheng Shi0Chengqing Hu1Yuan Zhou2Chunmei Cui3Jichun Yang4Qinghua Cui5Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, ChinaDepartment of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, ChinaDepartment of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, ChinaDepartment of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, ChinaDepartment of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China; Corresponding author: Jichun Yang, Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China.Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China; Corresponding author: Qinghua Cui, Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China.Physiological and pathophysiological differences widely exist in paired organ systems. However, the molecular basis for these differences remains largely unknown. We previously reported that there exist differentially expressed miRNAs (DEMs) in the left and right kidneys of normal mice. Here, we identified the DEMs in the left and right eyes, lungs, and testes of normal mice via RNA sequencing. As a result, we identified 26 DEMs in eyes, with 23 higher and 3 lower in the left eyes compared with right eyes; 21 DEMs in lungs, with 15 higher and 6 lower in the left lungs compared with right lungs; and 54 DEMs in testes, with 6 higher and 48 lower in the left testes compared with right testes. Ten microRNAs (miRNAs) were further examined by quantitative PCR assays, and seven of these were confirmed. In addition, correlation analysis was performed between paired organ miRNA expressions and diverse body fluid miRNA expressions. Finally, we explored the functions and networks of DEMs and performed biological process and pathway enrichment analysis of target genes for DEMs, providing insights into the physiological and pathophysiological differences between the two entities of paired organs.http://www.sciencedirect.com/science/article/pii/S2162253120301955miRNApaired organgene profilebioinformatic analysis |
| spellingShingle | Jiangcheng Shi Chengqing Hu Yuan Zhou Chunmei Cui Jichun Yang Qinghua Cui MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice Molecular Therapy: Nucleic Acids miRNA paired organ gene profile bioinformatic analysis |
| title | MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice |
| title_full | MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice |
| title_fullStr | MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice |
| title_full_unstemmed | MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice |
| title_short | MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice |
| title_sort | microrna profiling in paired left and right eyes lungs and testes of normal mice |
| topic | miRNA paired organ gene profile bioinformatic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2162253120301955 |
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