Elevated CO<sub>2</sub> Differentially Mitigated Oxidative Stress Induced by Indium Oxide Nanoparticles in Young and Old Leaves of C3 and C4 Crops
Soil contamination with indium (In) oxide nanoparticles (In<sub>2</sub>O<sub>3</sub>-NPs) threatens plant growth and development. However, their toxicity in plants under ambient (aCO<sub>2</sub>) and elevated (eCO<sub>2</sub>) conditions is scarcely st...
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MDPI AG
2022-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/2/308 |
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| author | Ibrahim I. Shabbaj Hamada AbdElgawad Mansour A. Balkhyour Abdurazag Tammar Mahmoud M. Y. Madany |
| author_facet | Ibrahim I. Shabbaj Hamada AbdElgawad Mansour A. Balkhyour Abdurazag Tammar Mahmoud M. Y. Madany |
| author_sort | Ibrahim I. Shabbaj |
| collection | DOAJ |
| description | Soil contamination with indium (In) oxide nanoparticles (In<sub>2</sub>O<sub>3</sub>-NPs) threatens plant growth and development. However, their toxicity in plants under ambient (aCO<sub>2</sub>) and elevated (eCO<sub>2</sub>) conditions is scarcely studied. To this end, this study was conducted to investigate In<sub>2</sub>O<sub>3</sub>-NPs toxicity in the young and old leaves of C3 (barley) and C4 (maize) plants and to understand the mechanisms underlying the stress mitigating impact of eCO<sub>2</sub>. Treatment of C3 and C4 plants with In<sub>2</sub>O<sub>3</sub>-NPs significantly reduced growth and photosynthesis, induced oxidative damage (H<sub>2</sub>O<sub>2</sub>, lipid peroxidation), and impaired P and Fe homeostasis, particularly in the young leaves of C4 plants. On the other hand, this phytotoxic hazard was mitigated by eCO<sub>2</sub> which improved both C3 and C4 growth, decreased In accumulation and increased phosphorus (P) and iron (Fe) uptake, particularly in the young leaves of C4 plants. Moreover, the improved photosynthesis by eCO<sub>2</sub> accordingly enhanced carbon availability under the challenge of In<sub>2</sub>O<sub>3</sub>-NPs that were directed to the elevated production of metabolites involved in antioxidant and detoxification systems. Our physiological and biochemical analyses implicated the role of the antioxidant defenses, including superoxide dismutase (SOD) in stress mitigation under eCO<sub>2</sub>. This was validated by studying the effect of In<sub>2</sub>O<sub>3</sub>-stress on a transgenic maize line (TG) constitutively overexpressing the <i>AtFeSOD</i> gene and its wild type (WT). Although it did not alter In accumulation, the TG plants showed improved growth and photosynthesis and reduced oxidative damage. Overall, this work demonstrated that C3 was more sensitive to In<sub>2</sub>O<sub>3</sub>-NPs stress; however, C4 plants were more responsive to eCO<sub>2</sub>. Moreover, it demonstrated the role of SOD in determining the hazardous effect of In<sub>2</sub>O<sub>3</sub>-NPs. |
| first_indexed | 2024-03-09T22:44:32Z |
| format | Article |
| id | doaj.art-68fcfd75b0694ebd94e9fadaaca9bae0 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T22:44:32Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-68fcfd75b0694ebd94e9fadaaca9bae02023-11-23T18:31:43ZengMDPI AGAntioxidants2076-39212022-02-0111230810.3390/antiox11020308Elevated CO<sub>2</sub> Differentially Mitigated Oxidative Stress Induced by Indium Oxide Nanoparticles in Young and Old Leaves of C3 and C4 CropsIbrahim I. Shabbaj0Hamada AbdElgawad1Mansour A. Balkhyour2Abdurazag Tammar3Mahmoud M. Y. Madany4Department of Environmental Sciences, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21441, Saudi ArabiaDepartment of Botany, Faculty of Science, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Environmental Sciences, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21441, Saudi ArabiaDepartment of Environmental Sciences, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21441, Saudi ArabiaDepartment of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, EgyptSoil contamination with indium (In) oxide nanoparticles (In<sub>2</sub>O<sub>3</sub>-NPs) threatens plant growth and development. However, their toxicity in plants under ambient (aCO<sub>2</sub>) and elevated (eCO<sub>2</sub>) conditions is scarcely studied. To this end, this study was conducted to investigate In<sub>2</sub>O<sub>3</sub>-NPs toxicity in the young and old leaves of C3 (barley) and C4 (maize) plants and to understand the mechanisms underlying the stress mitigating impact of eCO<sub>2</sub>. Treatment of C3 and C4 plants with In<sub>2</sub>O<sub>3</sub>-NPs significantly reduced growth and photosynthesis, induced oxidative damage (H<sub>2</sub>O<sub>2</sub>, lipid peroxidation), and impaired P and Fe homeostasis, particularly in the young leaves of C4 plants. On the other hand, this phytotoxic hazard was mitigated by eCO<sub>2</sub> which improved both C3 and C4 growth, decreased In accumulation and increased phosphorus (P) and iron (Fe) uptake, particularly in the young leaves of C4 plants. Moreover, the improved photosynthesis by eCO<sub>2</sub> accordingly enhanced carbon availability under the challenge of In<sub>2</sub>O<sub>3</sub>-NPs that were directed to the elevated production of metabolites involved in antioxidant and detoxification systems. Our physiological and biochemical analyses implicated the role of the antioxidant defenses, including superoxide dismutase (SOD) in stress mitigation under eCO<sub>2</sub>. This was validated by studying the effect of In<sub>2</sub>O<sub>3</sub>-stress on a transgenic maize line (TG) constitutively overexpressing the <i>AtFeSOD</i> gene and its wild type (WT). Although it did not alter In accumulation, the TG plants showed improved growth and photosynthesis and reduced oxidative damage. Overall, this work demonstrated that C3 was more sensitive to In<sub>2</sub>O<sub>3</sub>-NPs stress; however, C4 plants were more responsive to eCO<sub>2</sub>. Moreover, it demonstrated the role of SOD in determining the hazardous effect of In<sub>2</sub>O<sub>3</sub>-NPs.https://www.mdpi.com/2076-3921/11/2/308elevated CO<sub>2</sub><i>Hordeum vulgare</i><i>Zea maize</i>ROS homeostasisheavy metaldetoxification metabolism |
| spellingShingle | Ibrahim I. Shabbaj Hamada AbdElgawad Mansour A. Balkhyour Abdurazag Tammar Mahmoud M. Y. Madany Elevated CO<sub>2</sub> Differentially Mitigated Oxidative Stress Induced by Indium Oxide Nanoparticles in Young and Old Leaves of C3 and C4 Crops Antioxidants elevated CO<sub>2</sub> <i>Hordeum vulgare</i> <i>Zea maize</i> ROS homeostasis heavy metal detoxification metabolism |
| title | Elevated CO<sub>2</sub> Differentially Mitigated Oxidative Stress Induced by Indium Oxide Nanoparticles in Young and Old Leaves of C3 and C4 Crops |
| title_full | Elevated CO<sub>2</sub> Differentially Mitigated Oxidative Stress Induced by Indium Oxide Nanoparticles in Young and Old Leaves of C3 and C4 Crops |
| title_fullStr | Elevated CO<sub>2</sub> Differentially Mitigated Oxidative Stress Induced by Indium Oxide Nanoparticles in Young and Old Leaves of C3 and C4 Crops |
| title_full_unstemmed | Elevated CO<sub>2</sub> Differentially Mitigated Oxidative Stress Induced by Indium Oxide Nanoparticles in Young and Old Leaves of C3 and C4 Crops |
| title_short | Elevated CO<sub>2</sub> Differentially Mitigated Oxidative Stress Induced by Indium Oxide Nanoparticles in Young and Old Leaves of C3 and C4 Crops |
| title_sort | elevated co sub 2 sub differentially mitigated oxidative stress induced by indium oxide nanoparticles in young and old leaves of c3 and c4 crops |
| topic | elevated CO<sub>2</sub> <i>Hordeum vulgare</i> <i>Zea maize</i> ROS homeostasis heavy metal detoxification metabolism |
| url | https://www.mdpi.com/2076-3921/11/2/308 |
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