<i>Streptomyces</i> sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases
Bacteria and fungi are major phytopathogens which substantially affect global agricultural productivity. In the present study, <i>Streptomyces</i> sp. AN090126, isolated from agricultural suppressive soil in Korea, showed broad-spectrum antagonistic activity against various phytopathogen...
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/791 |
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| author | Khanh Duy Le Nan Hee Yu Ae Ran Park Dong-Jin Park Chang-Jin Kim Jin-Cheol Kim |
| author_facet | Khanh Duy Le Nan Hee Yu Ae Ran Park Dong-Jin Park Chang-Jin Kim Jin-Cheol Kim |
| author_sort | Khanh Duy Le |
| collection | DOAJ |
| description | Bacteria and fungi are major phytopathogens which substantially affect global agricultural productivity. In the present study, <i>Streptomyces</i> sp. AN090126, isolated from agricultural suppressive soil in Korea, showed broad-spectrum antagonistic activity against various phytopathogenic bacteria and fungi. In the 96-well plate assay, the fermentation filtrate of <i>Streptomyces</i> sp. AN090126 exhibited antimicrobial activity, with a minimum inhibitory concentration (MIC) of 0.63–10% for bacteria and 0.63–3.3% for fungi. The MIC of the partially purified fraction was 20.82–250 µg/mL for bacteria and 15.6–83.33 µg/mL for fungi. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that AN090126 produced various volatile organic compounds (VOCs), including dimethyl sulfide and trimethyl sulfide, which inhibited the growth of pathogenic bacteria and fungi in in vitro VOC assays. In pot experiments, the fermentation broth of <i>Streptomyces</i> sp. AN090126 reduced tomato bacterial wilt caused by <i>Ralstonia solanacearum</i>, red pepper leaf spot caused by <i>Xanthomonas euvesicatoria</i>, and creeping bentgrass dollar spot caused by <i>Sclerotinia homoeocarpa</i> in a dose-dependent manner. Moreover, the secondary metabolites derived from this strain showed a synergistic effect with streptomycin sulfate against streptomycin-resistant <i>Pectobacterium carotovorum</i> subsp. <i>carotovorum</i>, the causative agent of Kimchi cabbage soft rot, in both in vitro and in vivo experiments. Therefore, <i>Streptomyces</i> sp. AN090126 is a potential biocontrol agent in controlling plant diseases caused by pathogenic bacteria and fungi, specifically by the streptomycin-resistant strains. |
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| language | English |
| last_indexed | 2024-03-09T10:31:09Z |
| publishDate | 2022-04-01 |
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| series | Microorganisms |
| spelling | doaj.art-68fd39d85b22441590eaf5111fe008512023-12-01T21:15:18ZengMDPI AGMicroorganisms2076-26072022-04-0110479110.3390/microorganisms10040791<i>Streptomyces</i> sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant DiseasesKhanh Duy Le0Nan Hee Yu1Ae Ran Park2Dong-Jin Park3Chang-Jin Kim4Jin-Cheol Kim5Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonam National University, Gwangju 61186, KoreaDepartment of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonam National University, Gwangju 61186, KoreaDepartment of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonam National University, Gwangju 61186, KoreaIndustrial Bio-Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, KoreaIndustrial Bio-Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, KoreaDepartment of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonam National University, Gwangju 61186, KoreaBacteria and fungi are major phytopathogens which substantially affect global agricultural productivity. In the present study, <i>Streptomyces</i> sp. AN090126, isolated from agricultural suppressive soil in Korea, showed broad-spectrum antagonistic activity against various phytopathogenic bacteria and fungi. In the 96-well plate assay, the fermentation filtrate of <i>Streptomyces</i> sp. AN090126 exhibited antimicrobial activity, with a minimum inhibitory concentration (MIC) of 0.63–10% for bacteria and 0.63–3.3% for fungi. The MIC of the partially purified fraction was 20.82–250 µg/mL for bacteria and 15.6–83.33 µg/mL for fungi. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that AN090126 produced various volatile organic compounds (VOCs), including dimethyl sulfide and trimethyl sulfide, which inhibited the growth of pathogenic bacteria and fungi in in vitro VOC assays. In pot experiments, the fermentation broth of <i>Streptomyces</i> sp. AN090126 reduced tomato bacterial wilt caused by <i>Ralstonia solanacearum</i>, red pepper leaf spot caused by <i>Xanthomonas euvesicatoria</i>, and creeping bentgrass dollar spot caused by <i>Sclerotinia homoeocarpa</i> in a dose-dependent manner. Moreover, the secondary metabolites derived from this strain showed a synergistic effect with streptomycin sulfate against streptomycin-resistant <i>Pectobacterium carotovorum</i> subsp. <i>carotovorum</i>, the causative agent of Kimchi cabbage soft rot, in both in vitro and in vivo experiments. Therefore, <i>Streptomyces</i> sp. AN090126 is a potential biocontrol agent in controlling plant diseases caused by pathogenic bacteria and fungi, specifically by the streptomycin-resistant strains.https://www.mdpi.com/2076-2607/10/4/791<i>Streptomyces</i>antimicrobial activitybiocontrol agentVOCssynergistic effect |
| spellingShingle | Khanh Duy Le Nan Hee Yu Ae Ran Park Dong-Jin Park Chang-Jin Kim Jin-Cheol Kim <i>Streptomyces</i> sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases Microorganisms <i>Streptomyces</i> antimicrobial activity biocontrol agent VOCs synergistic effect |
| title | <i>Streptomyces</i> sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases |
| title_full | <i>Streptomyces</i> sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases |
| title_fullStr | <i>Streptomyces</i> sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases |
| title_full_unstemmed | <i>Streptomyces</i> sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases |
| title_short | <i>Streptomyces</i> sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases |
| title_sort | i streptomyces i sp an090126 as a biocontrol agent against bacterial and fungal plant diseases |
| topic | <i>Streptomyces</i> antimicrobial activity biocontrol agent VOCs synergistic effect |
| url | https://www.mdpi.com/2076-2607/10/4/791 |
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