3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion
OBJECTIVES/SPECIFIC AIMS: To evaluate the ability of various techniques to track changes in body fluid volumes before and after a rapid infusion of saline. METHODS/STUDY POPULATION: Eight healthy participants (5M; 3F) completed baseline measurements of 1) total body water using ethanol dilution and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2019-03-01
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| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866119001158/type/journal_article |
| _version_ | 1827995333779521536 |
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| author | Kevin Lawrence Kelly Alex R. Carlson Bradley B. Cierzan Jennifer Isautier Wayne L. Miller Bruce D. Johnson |
| author_facet | Kevin Lawrence Kelly Alex R. Carlson Bradley B. Cierzan Jennifer Isautier Wayne L. Miller Bruce D. Johnson |
| author_sort | Kevin Lawrence Kelly |
| collection | DOAJ |
| description | OBJECTIVES/SPECIFIC AIMS: To evaluate the ability of various techniques to track changes in body fluid volumes before and after a rapid infusion of saline. METHODS/STUDY POPULATION: Eight healthy participants (5M; 3F) completed baseline measurements of 1) total body water using ethanol dilution and bioelectrical impedance analysis (BIA) and 2) blood volume, plasma volume and red blood cell (RBC) volume using carbon monoxide rebreathe technique and I-131 albumin dilution. Subsequently, 30mL saline/kg body weight was administered intravenously over 20 minutes after which BIA and ethanol dilution were repeated. RESULTS/ANTICIPATED RESULTS: On average, 2.29±0.35 L saline was infused with an average increase in net fluid input-output (I/O) of 1.56±0.29 L. BIA underestimated measured I/O by −3.4±7.9%, while ethanol dilution did not demonstrate a measurable change in total body water. Carbon monoxide rebreathe differed from I-131 albumin dilution measurements of blood, plasma and RBC volumes by +0.6±2.8%, −5.4±3.6%, and +11.0±4.7%, respectively. DISCUSSION/SIGNIFICANCE OF IMPACT: BIA is capable of tracking modest changes in total body water. Carbon monoxide rebreathe appears to be a viable alternative for the I-131 albumin dilution technique to determine blood volume. Together, these two techniques may be useful in monitoring fluid status in patients with impaired fluid regulation. |
| first_indexed | 2024-04-10T04:53:41Z |
| format | Article |
| id | doaj.art-6902e2ac7a4c4f688972fe4f39108dca |
| institution | Directory Open Access Journal |
| issn | 2059-8661 |
| language | English |
| last_indexed | 2024-04-10T04:53:41Z |
| publishDate | 2019-03-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj.art-6902e2ac7a4c4f688972fe4f39108dca2023-03-09T12:30:30ZengCambridge University PressJournal of Clinical and Translational Science2059-86612019-03-013484910.1017/cts.2019.1153092 Measuring Fluid Compartments Before and After Rapid Saline InfusionKevin Lawrence Kelly0Alex R. Carlson1Bradley B. Cierzan2Jennifer Isautier3Wayne L. Miller4Bruce D. Johnson5Mayo ClinicMayo ClinicMayo ClinicMayo ClinicMayo ClinicMayo ClinicOBJECTIVES/SPECIFIC AIMS: To evaluate the ability of various techniques to track changes in body fluid volumes before and after a rapid infusion of saline. METHODS/STUDY POPULATION: Eight healthy participants (5M; 3F) completed baseline measurements of 1) total body water using ethanol dilution and bioelectrical impedance analysis (BIA) and 2) blood volume, plasma volume and red blood cell (RBC) volume using carbon monoxide rebreathe technique and I-131 albumin dilution. Subsequently, 30mL saline/kg body weight was administered intravenously over 20 minutes after which BIA and ethanol dilution were repeated. RESULTS/ANTICIPATED RESULTS: On average, 2.29±0.35 L saline was infused with an average increase in net fluid input-output (I/O) of 1.56±0.29 L. BIA underestimated measured I/O by −3.4±7.9%, while ethanol dilution did not demonstrate a measurable change in total body water. Carbon monoxide rebreathe differed from I-131 albumin dilution measurements of blood, plasma and RBC volumes by +0.6±2.8%, −5.4±3.6%, and +11.0±4.7%, respectively. DISCUSSION/SIGNIFICANCE OF IMPACT: BIA is capable of tracking modest changes in total body water. Carbon monoxide rebreathe appears to be a viable alternative for the I-131 albumin dilution technique to determine blood volume. Together, these two techniques may be useful in monitoring fluid status in patients with impaired fluid regulation.https://www.cambridge.org/core/product/identifier/S2059866119001158/type/journal_article |
| spellingShingle | Kevin Lawrence Kelly Alex R. Carlson Bradley B. Cierzan Jennifer Isautier Wayne L. Miller Bruce D. Johnson 3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion Journal of Clinical and Translational Science |
| title | 3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion |
| title_full | 3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion |
| title_fullStr | 3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion |
| title_full_unstemmed | 3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion |
| title_short | 3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion |
| title_sort | 3092 measuring fluid compartments before and after rapid saline infusion |
| url | https://www.cambridge.org/core/product/identifier/S2059866119001158/type/journal_article |
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