The Molecular Characterization of <i>bla</i><sub>NDM-1</sub>-Positive <i>Acinetobacter baumannii</i> Isolated in Central Greece
The objective of the present study is to report the detection and the molecular characterization of nine <i>bla</i><sub>NDM-1</sub>-positive <i>Acinetobacter baumannii</i> isolates, which were isolated from patients in a tertiary care hospital in Central Greece fr...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2588 |
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| author | Katerina Tsilipounidaki Christos-George Gkountinoudis Zoi Florou George C. Fthenakis Vivi Miriagou Efthymia Petinaki |
| author_facet | Katerina Tsilipounidaki Christos-George Gkountinoudis Zoi Florou George C. Fthenakis Vivi Miriagou Efthymia Petinaki |
| author_sort | Katerina Tsilipounidaki |
| collection | DOAJ |
| description | The objective of the present study is to report the detection and the molecular characterization of nine <i>bla</i><sub>NDM-1</sub>-positive <i>Acinetobacter baumannii</i> isolates, which were isolated from patients in a tertiary care hospital in Central Greece from December 2022 to August 2023. The isolates were characterized by whole genome sequencing to obtain Pasteur multilocus sequencing typing (MLST) and to identify the <i>bla</i><sub>NDM-1</sub>-environment, resistome, and virulence genes content. In silico MLST analysis showed that the isolates belonged to four different clones (STs 160, 2, 85, and 2493). All strains, apart from the <i>bla</i><sub>NDM-1</sub>-gene, possessed at least eight different genes, encoding resistance to various antimicrobial agents. Whole genome sequencing revealed two different structures of the <i>bla</i><sub>NDM-1</sub> environment. The first, detected in ST160 strain, was identical with the Tn<i>125</i>, whereas the second, found in STs 2, 85, and 2493 was associated with Tn<i>7382.</i> To our knowledge, after a sole strain reported in 2016 and imported by a patient hospitalized in a Libyan hospital, this is the first report of the emergence of polyclonal <i>bla</i><sub>NDM-1</sub>-positive <i>Acinetobacter baumannii</i> in Greece. Our findings re-emphasize the need to apply diligent surveillance protocols in order to limit the horizontal transfer of the <i>bla</i><sub>NDM-1</sub> gene to other <i>A. baumannii</i> clones or to other recipient strains. |
| first_indexed | 2024-03-10T21:01:38Z |
| format | Article |
| id | doaj.art-6906a7441211435f8074c1a67ff8c3e2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T21:01:38Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6906a7441211435f8074c1a67ff8c3e22023-11-19T17:29:03ZengMDPI AGMicroorganisms2076-26072023-10-011110258810.3390/microorganisms11102588The Molecular Characterization of <i>bla</i><sub>NDM-1</sub>-Positive <i>Acinetobacter baumannii</i> Isolated in Central GreeceKaterina Tsilipounidaki0Christos-George Gkountinoudis1Zoi Florou2George C. Fthenakis3Vivi Miriagou4Efthymia Petinaki5Faculty of Medicine, University of Thessaly, 41500 Larissa, GreeceFaculty of Medicine, University of Thessaly, 41500 Larissa, GreeceFaculty of Medicine, University of Thessaly, 41500 Larissa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceLaboratory of Bacteriology, Hellenic Pasteur Institute, 11521 Athens, GreeceFaculty of Medicine, University of Thessaly, 41500 Larissa, GreeceThe objective of the present study is to report the detection and the molecular characterization of nine <i>bla</i><sub>NDM-1</sub>-positive <i>Acinetobacter baumannii</i> isolates, which were isolated from patients in a tertiary care hospital in Central Greece from December 2022 to August 2023. The isolates were characterized by whole genome sequencing to obtain Pasteur multilocus sequencing typing (MLST) and to identify the <i>bla</i><sub>NDM-1</sub>-environment, resistome, and virulence genes content. In silico MLST analysis showed that the isolates belonged to four different clones (STs 160, 2, 85, and 2493). All strains, apart from the <i>bla</i><sub>NDM-1</sub>-gene, possessed at least eight different genes, encoding resistance to various antimicrobial agents. Whole genome sequencing revealed two different structures of the <i>bla</i><sub>NDM-1</sub> environment. The first, detected in ST160 strain, was identical with the Tn<i>125</i>, whereas the second, found in STs 2, 85, and 2493 was associated with Tn<i>7382.</i> To our knowledge, after a sole strain reported in 2016 and imported by a patient hospitalized in a Libyan hospital, this is the first report of the emergence of polyclonal <i>bla</i><sub>NDM-1</sub>-positive <i>Acinetobacter baumannii</i> in Greece. Our findings re-emphasize the need to apply diligent surveillance protocols in order to limit the horizontal transfer of the <i>bla</i><sub>NDM-1</sub> gene to other <i>A. baumannii</i> clones or to other recipient strains.https://www.mdpi.com/2076-2607/11/10/2588<i>Acinetobacter baumannii</i>antibiotic resistance<i>bla</i><sub>NDM-1</sub>carbapenemGreece |
| spellingShingle | Katerina Tsilipounidaki Christos-George Gkountinoudis Zoi Florou George C. Fthenakis Vivi Miriagou Efthymia Petinaki The Molecular Characterization of <i>bla</i><sub>NDM-1</sub>-Positive <i>Acinetobacter baumannii</i> Isolated in Central Greece Microorganisms <i>Acinetobacter baumannii</i> antibiotic resistance <i>bla</i><sub>NDM-1</sub> carbapenem Greece |
| title | The Molecular Characterization of <i>bla</i><sub>NDM-1</sub>-Positive <i>Acinetobacter baumannii</i> Isolated in Central Greece |
| title_full | The Molecular Characterization of <i>bla</i><sub>NDM-1</sub>-Positive <i>Acinetobacter baumannii</i> Isolated in Central Greece |
| title_fullStr | The Molecular Characterization of <i>bla</i><sub>NDM-1</sub>-Positive <i>Acinetobacter baumannii</i> Isolated in Central Greece |
| title_full_unstemmed | The Molecular Characterization of <i>bla</i><sub>NDM-1</sub>-Positive <i>Acinetobacter baumannii</i> Isolated in Central Greece |
| title_short | The Molecular Characterization of <i>bla</i><sub>NDM-1</sub>-Positive <i>Acinetobacter baumannii</i> Isolated in Central Greece |
| title_sort | molecular characterization of i bla i sub ndm 1 sub positive i acinetobacter baumannii i isolated in central greece |
| topic | <i>Acinetobacter baumannii</i> antibiotic resistance <i>bla</i><sub>NDM-1</sub> carbapenem Greece |
| url | https://www.mdpi.com/2076-2607/11/10/2588 |
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