Microfluidics for detection of exosomes and microRNAs in cancer: State of the art
Exosomes are small extracellular vesicles with sizes ranging from 30–150 nanometers that contain proteins, lipids, mRNAs, microRNAs, and double-stranded DNA derived from the cells of origin. Exosomes can be taken up by target cells, acting as a means of cell-to-cell communication. The discovery of t...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253122000877 |
| _version_ | 1817980450586492928 |
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| author | Seyed Mojtaba Mousavi Seyed Mohammad Amin Mahdian Mohammad Saeid Ebrahimi Mohammad Taghizadieh Massoud Vosough Javid Sadri Nahand Saereh Hosseindoost Nasim Vousooghi Hamid Akbari Javar Bagher Larijani Mahmoud Reza Hadjighassem Neda Rahimian Michael R. Hamblin Hamed Mirzaei |
| author_facet | Seyed Mojtaba Mousavi Seyed Mohammad Amin Mahdian Mohammad Saeid Ebrahimi Mohammad Taghizadieh Massoud Vosough Javid Sadri Nahand Saereh Hosseindoost Nasim Vousooghi Hamid Akbari Javar Bagher Larijani Mahmoud Reza Hadjighassem Neda Rahimian Michael R. Hamblin Hamed Mirzaei |
| author_sort | Seyed Mojtaba Mousavi |
| collection | DOAJ |
| description | Exosomes are small extracellular vesicles with sizes ranging from 30–150 nanometers that contain proteins, lipids, mRNAs, microRNAs, and double-stranded DNA derived from the cells of origin. Exosomes can be taken up by target cells, acting as a means of cell-to-cell communication. The discovery of these vesicles in body fluids and their participation in cell communication has led to major breakthroughs in diagnosis, prognosis, and treatment of several conditions (e.g., cancer). However, conventional isolation and evaluation of exosomes and their microRNA content suffers from high cost, lengthy processes, difficult standardization, low purity, and poor yield. The emergence of microfluidics devices with increased efficiency in sieving, trapping, and immunological separation of small volumes could provide improved detection and monitoring of exosomes involved in cancer. Microfluidics techniques hold promise for advances in development of diagnostic and prognostic devices. This review covers ongoing research on microfluidics devices for detection of microRNAs and exosomes as biomarkers and their translation to point-of-care and clinical applications. |
| first_indexed | 2024-04-13T22:55:00Z |
| format | Article |
| id | doaj.art-69089555b8dd495eb00e912a3272d501 |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-04-13T22:55:00Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-69089555b8dd495eb00e912a3272d5012022-12-22T02:26:04ZengElsevierMolecular Therapy: Nucleic Acids2162-25312022-06-0128758791Microfluidics for detection of exosomes and microRNAs in cancer: State of the artSeyed Mojtaba Mousavi0Seyed Mohammad Amin Mahdian1Mohammad Saeid Ebrahimi2Mohammad Taghizadieh3Massoud Vosough4Javid Sadri Nahand5Saereh Hosseindoost6Nasim Vousooghi7Hamid Akbari Javar8Bagher Larijani9Mahmoud Reza Hadjighassem10Neda Rahimian11Michael R. Hamblin12Hamed Mirzaei13Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, IranSchool of Medicine, Kashan University of Medical Sciences, Kashan, Iran; Student Research Committee, Kashan University of Medical Sciences, Kashan, IranDepartment of Pathology, School of Medicine, Center for Women’s Health Research Zahra, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran 1665659911, IranInfectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranPain Research Center, Neuroscience Institute, Tehran University of Medical Science, Tehran, IranDepartment of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Cognitive and Behavioral Sciences, Tehran University of Medical Sciences, Tehran, Iran; Iranian National Center for Addiction Studies (INCAS), Tehran University of Medical Sciences, Tehran, IranEndocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran; Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranEndocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Brain and Spinal Cord Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran; Corresponding author Mahmoud Reza Hadjighassem, Brain and Spinal Cord Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS), Tehran, Iran; Corresponding author Neda Rahimian, Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS), Tehran, Iran.Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein 2028, South Africa; Corresponding author Michael R. Hamblin, Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein 2028, South Africa.Research Center for Biochemistry and Nutrition in Metabolic Diseases, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran; Corresponding author Hamed Mirzaei, Research Center for Biochemistry and Nutrition in Metabolic Diseases, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran.Exosomes are small extracellular vesicles with sizes ranging from 30–150 nanometers that contain proteins, lipids, mRNAs, microRNAs, and double-stranded DNA derived from the cells of origin. Exosomes can be taken up by target cells, acting as a means of cell-to-cell communication. The discovery of these vesicles in body fluids and their participation in cell communication has led to major breakthroughs in diagnosis, prognosis, and treatment of several conditions (e.g., cancer). However, conventional isolation and evaluation of exosomes and their microRNA content suffers from high cost, lengthy processes, difficult standardization, low purity, and poor yield. The emergence of microfluidics devices with increased efficiency in sieving, trapping, and immunological separation of small volumes could provide improved detection and monitoring of exosomes involved in cancer. Microfluidics techniques hold promise for advances in development of diagnostic and prognostic devices. This review covers ongoing research on microfluidics devices for detection of microRNAs and exosomes as biomarkers and their translation to point-of-care and clinical applications.http://www.sciencedirect.com/science/article/pii/S2162253122000877microRNAexosomesmicrofluidicsbiomarkerscancer |
| spellingShingle | Seyed Mojtaba Mousavi Seyed Mohammad Amin Mahdian Mohammad Saeid Ebrahimi Mohammad Taghizadieh Massoud Vosough Javid Sadri Nahand Saereh Hosseindoost Nasim Vousooghi Hamid Akbari Javar Bagher Larijani Mahmoud Reza Hadjighassem Neda Rahimian Michael R. Hamblin Hamed Mirzaei Microfluidics for detection of exosomes and microRNAs in cancer: State of the art Molecular Therapy: Nucleic Acids microRNA exosomes microfluidics biomarkers cancer |
| title | Microfluidics for detection of exosomes and microRNAs in cancer: State of the art |
| title_full | Microfluidics for detection of exosomes and microRNAs in cancer: State of the art |
| title_fullStr | Microfluidics for detection of exosomes and microRNAs in cancer: State of the art |
| title_full_unstemmed | Microfluidics for detection of exosomes and microRNAs in cancer: State of the art |
| title_short | Microfluidics for detection of exosomes and microRNAs in cancer: State of the art |
| title_sort | microfluidics for detection of exosomes and micrornas in cancer state of the art |
| topic | microRNA exosomes microfluidics biomarkers cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2162253122000877 |
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