Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonella
An essential factor to the virulence of the genus Candida is the ability to produce enzymes and this may be crucial in the establishment of fungal infections. Aim: This study investigated in vitro enzymatic activities of Candida species and their virulence in an in vivo Galleria mellonella experimen...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Campinas
2015-10-01
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| Series: | Brazilian Journal of Oral Sciences |
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| Online Access: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641030 |
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| author | Rodnei Dennis Rossoni Júnia Oliveira Barbosa Simone Furgeri Godinho Vilela Jéssica Diane dos Santos Antonio Olavo Cardoso Jorge Juliana Campos Junqueira |
| author_facet | Rodnei Dennis Rossoni Júnia Oliveira Barbosa Simone Furgeri Godinho Vilela Jéssica Diane dos Santos Antonio Olavo Cardoso Jorge Juliana Campos Junqueira |
| author_sort | Rodnei Dennis Rossoni |
| collection | DOAJ |
| description | An essential factor to the virulence of the genus Candida is the ability to produce enzymes and this may be crucial in the establishment of fungal infections. Aim: This study investigated in vitro enzymatic activities of Candida species and their virulence in an in vivo Galleria mellonella experimental model. Methods: Twenty-four clinical strains of Candida spp. isolated from the human oral cavity were evaluated, including the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. norvegensis, C. lusitaniae and C. guilliermondii. All Candida strains were tested in vitro for production of proteinase and phospholipase. The Candida strains were also injected into Galleria mellonella larvae to induce experimental candidiasis, and after 24 hours, the survival rate was assessed. Results: Phospholipase and proteinase activity were observed in 100% of the C. albicans strains. In the non-albicans species, proteinase and phospholipase activity were observed in 25 and 43% of the studied strains, respectively. The most pathogenic Candida species in G. mellonella were C. albicans, C. dubliniensis and C. lusitaniae, whereas C. glabrata was the least virulent species. Furthermore, a positive significant correlation was found between both enzymatic activities with virulence in G. mellonella. Conclusions: The virulence of Candida strains in G. mellonella is related to the quantity of proteinases and phospholipases production of each strain. |
| first_indexed | 2024-12-22T02:29:36Z |
| format | Article |
| id | doaj.art-69091553e3ae41e0b1bd0f91a58acae8 |
| institution | Directory Open Access Journal |
| issn | 1677-3225 |
| language | English |
| last_indexed | 2024-12-22T02:29:36Z |
| publishDate | 2015-10-01 |
| publisher | Universidade Estadual de Campinas |
| record_format | Article |
| series | Brazilian Journal of Oral Sciences |
| spelling | doaj.art-69091553e3ae41e0b1bd0f91a58acae82022-12-21T18:41:55ZengUniversidade Estadual de CampinasBrazilian Journal of Oral Sciences1677-32252015-10-0112310.20396/bjos.v12i3.8641030Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonellaRodnei Dennis Rossoni0Júnia Oliveira Barbosa1Simone Furgeri Godinho Vilela2Jéssica Diane dos Santos3Antonio Olavo Cardoso Jorge4Juliana Campos Junqueira5Univ Estadual Paulista, UNESPUniv Estadual Paulista, UNESPUniv Estadual Paulista, UNESPUniv Estadual Paulista, UNESPUniv Estadual Paulista, UNESPUniv Estadual Paulista, UNESPAn essential factor to the virulence of the genus Candida is the ability to produce enzymes and this may be crucial in the establishment of fungal infections. Aim: This study investigated in vitro enzymatic activities of Candida species and their virulence in an in vivo Galleria mellonella experimental model. Methods: Twenty-four clinical strains of Candida spp. isolated from the human oral cavity were evaluated, including the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. norvegensis, C. lusitaniae and C. guilliermondii. All Candida strains were tested in vitro for production of proteinase and phospholipase. The Candida strains were also injected into Galleria mellonella larvae to induce experimental candidiasis, and after 24 hours, the survival rate was assessed. Results: Phospholipase and proteinase activity were observed in 100% of the C. albicans strains. In the non-albicans species, proteinase and phospholipase activity were observed in 25 and 43% of the studied strains, respectively. The most pathogenic Candida species in G. mellonella were C. albicans, C. dubliniensis and C. lusitaniae, whereas C. glabrata was the least virulent species. Furthermore, a positive significant correlation was found between both enzymatic activities with virulence in G. mellonella. Conclusions: The virulence of Candida strains in G. mellonella is related to the quantity of proteinases and phospholipases production of each strain.https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641030CandidaVirulence factorsInvertebrates |
| spellingShingle | Rodnei Dennis Rossoni Júnia Oliveira Barbosa Simone Furgeri Godinho Vilela Jéssica Diane dos Santos Antonio Olavo Cardoso Jorge Juliana Campos Junqueira Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonella Brazilian Journal of Oral Sciences Candida Virulence factors Invertebrates |
| title | Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonella |
| title_full | Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonella |
| title_fullStr | Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonella |
| title_full_unstemmed | Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonella |
| title_short | Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonella |
| title_sort | correlation of phospholipase and proteinase production of candida with in vivo pathogenicity in galleria mellonella |
| topic | Candida Virulence factors Invertebrates |
| url | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641030 |
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