Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in Adenomyosis
Matrix metalloproteinases (MMPs) play an important role in menstruation and endometriosis; however, the membrane-type matrix metalloproteinases (MT-MMPs) are not well studied in endometriosis and adenomyosis. We analyzed MT2-MMP (MMP15) and MT3-MMP (MMP16) in eutopic endometrium with and without end...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/12/4/779 |
| _version_ | 1797436137495592960 |
|---|---|
| author | Jane B. Maoga Muhammad A. Riaz Agnes N. Mwaura Georgios Scheiner-Bobis Ezekiel Mecha Charles O. A. Omwandho Ivo Meinhold-Heerlein Lutz Konrad |
| author_facet | Jane B. Maoga Muhammad A. Riaz Agnes N. Mwaura Georgios Scheiner-Bobis Ezekiel Mecha Charles O. A. Omwandho Ivo Meinhold-Heerlein Lutz Konrad |
| author_sort | Jane B. Maoga |
| collection | DOAJ |
| description | Matrix metalloproteinases (MMPs) play an important role in menstruation and endometriosis; however, the membrane-type matrix metalloproteinases (MT-MMPs) are not well studied in endometriosis and adenomyosis. We analyzed MT2-MMP (MMP15) and MT3-MMP (MMP16) in eutopic endometrium with and without endometriosis and with and without adenomyosis and ectopic endometrium of deep infiltrating endometriosis (DIE), peritoneal endometriosis (PE), and ovarian endometriosis (Ov) by immunohistochemistry. Preferential expression of both proteins was observed in the glandular and luminal epithelial cells of the eutopic endometrium of patients with and without endometriosis with a ~2.5-fold stronger expression of MT3-MMP compared to MT2-MMP. We did not observe any differences during menstrual cycling and in eutopic endometrium of patients with and without endometriosis. Similarly, eutopic endometrium and adenomyotic tissue with and without endometriosis showed similar protein levels of MT2-MMP and MT3-MMP. In contrast, MT2-MMP and MT3-MMP protein was decreased in ectopic compared to eutopic endometrium and adenomyosis. The similar expression of MT2-MMP and MT3-MMP in eutopic endometrium in patients with and without endometriosis in contrast to the impaired expression in ectopic endometrium suggests that alterations occur after and not before endometrial implantation possibly by distinct interactions with the different environments. The differential protein expression of MT2/3-MMP in adenomyosis compared to endometriosis might suggest a different pathogenesis pathway for the two diseases. |
| first_indexed | 2024-03-09T10:58:13Z |
| format | Article |
| id | doaj.art-69095387a8244c3badad72034e9f6c1a |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T10:58:13Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-69095387a8244c3badad72034e9f6c1a2023-12-01T01:29:06ZengMDPI AGDiagnostics2075-44182022-03-0112477910.3390/diagnostics12040779Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in AdenomyosisJane B. Maoga0Muhammad A. Riaz1Agnes N. Mwaura2Georgios Scheiner-Bobis3Ezekiel Mecha4Charles O. A. Omwandho5Ivo Meinhold-Heerlein6Lutz Konrad7Department of Gynecology and Obstetrics, Justus Liebig University, 35392 Giessen, GermanyDepartment of Gynecology and Obstetrics, Justus Liebig University, 35392 Giessen, GermanyDepartment of Gynecology and Obstetrics, Justus Liebig University, 35392 Giessen, GermanyInstitute of Veterinary-Physiology and Biochemistry, Justus Liebig University, 35392 Giessen, GermanyDepartment of Biochemistry, University of Nairobi, Nairobi 00100, KenyaReproductive Biochemistry, Kirinyaga University, Kirinyaga 10300, KenyaDepartment of Gynecology and Obstetrics, Justus Liebig University, 35392 Giessen, GermanyDepartment of Gynecology and Obstetrics, Justus Liebig University, 35392 Giessen, GermanyMatrix metalloproteinases (MMPs) play an important role in menstruation and endometriosis; however, the membrane-type matrix metalloproteinases (MT-MMPs) are not well studied in endometriosis and adenomyosis. We analyzed MT2-MMP (MMP15) and MT3-MMP (MMP16) in eutopic endometrium with and without endometriosis and with and without adenomyosis and ectopic endometrium of deep infiltrating endometriosis (DIE), peritoneal endometriosis (PE), and ovarian endometriosis (Ov) by immunohistochemistry. Preferential expression of both proteins was observed in the glandular and luminal epithelial cells of the eutopic endometrium of patients with and without endometriosis with a ~2.5-fold stronger expression of MT3-MMP compared to MT2-MMP. We did not observe any differences during menstrual cycling and in eutopic endometrium of patients with and without endometriosis. Similarly, eutopic endometrium and adenomyotic tissue with and without endometriosis showed similar protein levels of MT2-MMP and MT3-MMP. In contrast, MT2-MMP and MT3-MMP protein was decreased in ectopic compared to eutopic endometrium and adenomyosis. The similar expression of MT2-MMP and MT3-MMP in eutopic endometrium in patients with and without endometriosis in contrast to the impaired expression in ectopic endometrium suggests that alterations occur after and not before endometrial implantation possibly by distinct interactions with the different environments. The differential protein expression of MT2/3-MMP in adenomyosis compared to endometriosis might suggest a different pathogenesis pathway for the two diseases.https://www.mdpi.com/2075-4418/12/4/779endometriumendometriosisadenomyosismembrane type-2 matrix metalloproteinasemembrane type-3 matrix metalloproteinase |
| spellingShingle | Jane B. Maoga Muhammad A. Riaz Agnes N. Mwaura Georgios Scheiner-Bobis Ezekiel Mecha Charles O. A. Omwandho Ivo Meinhold-Heerlein Lutz Konrad Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in Adenomyosis Diagnostics endometrium endometriosis adenomyosis membrane type-2 matrix metalloproteinase membrane type-3 matrix metalloproteinase |
| title | Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in Adenomyosis |
| title_full | Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in Adenomyosis |
| title_fullStr | Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in Adenomyosis |
| title_full_unstemmed | Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in Adenomyosis |
| title_short | Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in Adenomyosis |
| title_sort | impaired expression of membrane type 2 and type 3 matrix metalloproteinases in endometriosis but not in adenomyosis |
| topic | endometrium endometriosis adenomyosis membrane type-2 matrix metalloproteinase membrane type-3 matrix metalloproteinase |
| url | https://www.mdpi.com/2075-4418/12/4/779 |
| work_keys_str_mv | AT janebmaoga impairedexpressionofmembranetype2andtype3matrixmetalloproteinasesinendometriosisbutnotinadenomyosis AT muhammadariaz impairedexpressionofmembranetype2andtype3matrixmetalloproteinasesinendometriosisbutnotinadenomyosis AT agnesnmwaura impairedexpressionofmembranetype2andtype3matrixmetalloproteinasesinendometriosisbutnotinadenomyosis AT georgiosscheinerbobis impairedexpressionofmembranetype2andtype3matrixmetalloproteinasesinendometriosisbutnotinadenomyosis AT ezekielmecha impairedexpressionofmembranetype2andtype3matrixmetalloproteinasesinendometriosisbutnotinadenomyosis AT charlesoaomwandho impairedexpressionofmembranetype2andtype3matrixmetalloproteinasesinendometriosisbutnotinadenomyosis AT ivomeinholdheerlein impairedexpressionofmembranetype2andtype3matrixmetalloproteinasesinendometriosisbutnotinadenomyosis AT lutzkonrad impairedexpressionofmembranetype2andtype3matrixmetalloproteinasesinendometriosisbutnotinadenomyosis |