Acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar Atractosteus tropicus (Semionotiformes: Lepisosteidae)
Biomarkers are a widely applied approach in environmental studies. Analyses of cholinesterase (ChE), glutathione S-transferase (GST) and lipid peroxidation (LPO) are biomarkers that can provide information regarding early effects of pollutants at different biochemical levels on an organism. The aim...
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Vicerractoría Investigación
2012-03-01
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| Series: | Revista de Biología Tropical |
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| Online Access: | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442012000100025&lng=en&tlng=en |
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| author | Freylan Mena Torres Sascha Pfennig María de Jesús Arias Andrés Gabriel Márquez-Couturie Adrían Sevilla C. Maurizio Protti Q |
| author_facet | Freylan Mena Torres Sascha Pfennig María de Jesús Arias Andrés Gabriel Márquez-Couturie Adrían Sevilla C. Maurizio Protti Q |
| author_sort | Freylan Mena Torres |
| collection | DOAJ |
| description | Biomarkers are a widely applied approach in environmental studies. Analyses of cholinesterase (ChE), glutathione S-transferase (GST) and lipid peroxidation (LPO) are biomarkers that can provide information regarding early effects of pollutants at different biochemical levels on an organism. The aim of this study was to evaluate the biomarker approach on a Costa Rican native and relevant species. For this, larvae of gar (Atractosteus tropicus) were exposed to the organophosphorus nematicide, ethoprophos. Acute (96hr) exposure was conducted with pesticide concentrations ranging from 0.1μg/L to 1 500μg/L. The 96hr LC50 calculated was 859.7μg/L. After exposure, three biomarkers (ChE, GST and LPO) were analyzed in fish that survived the acute test. The lowest observed effect concentration (LOEC) regarding ChE activity inhibition was 50μg/L. This concentration produced a significant inhibition (p<0.05) of the enzyme by 20%. The highest concentration tested without showing any effect on ChE activity and therefore considered as no observed effect concentration (NOEC) was 10μg/L. Ethoprophos concentration of 400μg/L caused a ChE inhibition by 79%. In this study, no significant variations (p>0.05) in GST activity and LPO were observed in A. tropicus larvae after exposure to ethoprophos. |
| first_indexed | 2024-03-12T06:14:43Z |
| format | Article |
| id | doaj.art-690c0150f6d743bdb68334fc37b917f2 |
| institution | Directory Open Access Journal |
| issn | 0034-7744 |
| language | English |
| last_indexed | 2024-03-12T06:14:43Z |
| publishDate | 2012-03-01 |
| publisher | Vicerractoría Investigación |
| record_format | Article |
| series | Revista de Biología Tropical |
| spelling | doaj.art-690c0150f6d743bdb68334fc37b917f22023-09-03T02:39:52ZengVicerractoría InvestigaciónRevista de Biología Tropical0034-77442012-03-01601361368S0034-77442012000100025Acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar Atractosteus tropicus (Semionotiformes: Lepisosteidae)Freylan Mena Torres0Sascha Pfennig1María de Jesús Arias Andrés2Gabriel Márquez-Couturie3Adrían Sevilla4C. Maurizio Protti Q5Universidad Nacional de Costa RicaUniversidad Nacional de Costa RicaUniversidad Nacional de Costa RicaUniversidad Juárez Autónoma de TabascoUniversidad Nacional de Costa RicaUniversidad Nacional de Costa RicaBiomarkers are a widely applied approach in environmental studies. Analyses of cholinesterase (ChE), glutathione S-transferase (GST) and lipid peroxidation (LPO) are biomarkers that can provide information regarding early effects of pollutants at different biochemical levels on an organism. The aim of this study was to evaluate the biomarker approach on a Costa Rican native and relevant species. For this, larvae of gar (Atractosteus tropicus) were exposed to the organophosphorus nematicide, ethoprophos. Acute (96hr) exposure was conducted with pesticide concentrations ranging from 0.1μg/L to 1 500μg/L. The 96hr LC50 calculated was 859.7μg/L. After exposure, three biomarkers (ChE, GST and LPO) were analyzed in fish that survived the acute test. The lowest observed effect concentration (LOEC) regarding ChE activity inhibition was 50μg/L. This concentration produced a significant inhibition (p<0.05) of the enzyme by 20%. The highest concentration tested without showing any effect on ChE activity and therefore considered as no observed effect concentration (NOEC) was 10μg/L. Ethoprophos concentration of 400μg/L caused a ChE inhibition by 79%. In this study, no significant variations (p>0.05) in GST activity and LPO were observed in A. tropicus larvae after exposure to ethoprophos.http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442012000100025&lng=en&tlng=engasparAtractosteus tropicusetoprofosinhibidores de la colinesterasaglutatión S-transferasaperoxidación lipídica |
| spellingShingle | Freylan Mena Torres Sascha Pfennig María de Jesús Arias Andrés Gabriel Márquez-Couturie Adrían Sevilla C. Maurizio Protti Q Acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar Atractosteus tropicus (Semionotiformes: Lepisosteidae) Revista de Biología Tropical gaspar Atractosteus tropicus etoprofos inhibidores de la colinesterasa glutatión S-transferasa peroxidación lipídica |
| title | Acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar Atractosteus tropicus (Semionotiformes: Lepisosteidae) |
| title_full | Acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar Atractosteus tropicus (Semionotiformes: Lepisosteidae) |
| title_fullStr | Acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar Atractosteus tropicus (Semionotiformes: Lepisosteidae) |
| title_full_unstemmed | Acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar Atractosteus tropicus (Semionotiformes: Lepisosteidae) |
| title_short | Acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar Atractosteus tropicus (Semionotiformes: Lepisosteidae) |
| title_sort | acute toxicity and cholinesterase inhibition of the nematicide ethoprophos in larvae of gar atractosteus tropicus semionotiformes lepisosteidae |
| topic | gaspar Atractosteus tropicus etoprofos inhibidores de la colinesterasa glutatión S-transferasa peroxidación lipídica |
| url | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442012000100025&lng=en&tlng=en |
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