Assessment of genetic diversity and phylogenetic relationship of Limousin herds in Hungary using microsatellite markers
Objective This study was conducted to investigate basic information on genetic structure and characteristics of Limousin population in Hungary. Obtained results will be taken into consideration when adopting the new breeding strategy by the Association of Hungarian Limousin and Blonde d’Aquitaine Br...
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| Format: | Article |
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Asian-Australasian Association of Animal Production Societies
2019-02-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
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| Online Access: | http://www.ajas.info/upload/pdf/ajas-18-0164.pdf |
| _version_ | 1828241668084596736 |
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| author | Márton Szűcs Ferenc Szabó Beáta Bán Csilla Józsa László Rózsa Attila Zsolnai István Anton |
| author_facet | Márton Szűcs Ferenc Szabó Beáta Bán Csilla Józsa László Rózsa Attila Zsolnai István Anton |
| author_sort | Márton Szűcs |
| collection | DOAJ |
| description | Objective This study was conducted to investigate basic information on genetic structure and characteristics of Limousin population in Hungary. Obtained results will be taken into consideration when adopting the new breeding strategy by the Association of Hungarian Limousin and Blonde d’Aquitaine Breeders (AHLBB). Methods Genetic diversity and phylogenetic relationship of 3,443 Limousin cattle from 16 different herds were investigated by performing genotyping using 18 microsatellite markers. Amplified DNA was genotyped using an automated genetic analyzer. Results Mean of effective alleles (ne) of the populations was 3.77. Population C had the lowest number of effective alleles (3.01) and the lowest inbreeding coefficient (FIS) value (−0.15). Principal component analysis of estimated genetic distance (FST) values (p<0.000) revealed two herds (C and E) distinct from the majority of other Limousin herds. The pairwise FST values of population C compared to the others (0.066 to 0.120) fell into the range of moderate genetic distance: 0.050 to 0.150, while population E displayed also moderate genetic distance (FST values in range 0.052 to 0.064) but only to six populations (G, H, J, L, N, and P). FST(C-E) was 0.148, all other pairs -excluding C and E herds- displayed low genetic distance (FST<0.049). Population D, F, I, J, K, L, N, O, and P carried private alleles, which alleles belonged to 1.1% of the individuals. Most probable number of clusters (K) were 2 and 7 determined by Structure and BAPS software. Conclusion This study showed useful genetic diversity and phylogenetic relationship data that can be utilized for the development of a new breeding strategy by AHLBB. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Limousins. |
| first_indexed | 2024-04-12T22:02:53Z |
| format | Article |
| id | doaj.art-690ef86dc9ed4c4088843de80009e8d8 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-04-12T22:02:53Z |
| publishDate | 2019-02-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-690ef86dc9ed4c4088843de80009e8d82022-12-22T03:15:02ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172019-02-0132217618210.5713/ajas.18.016424039Assessment of genetic diversity and phylogenetic relationship of Limousin herds in Hungary using microsatellite markersMárton Szűcs0Ferenc Szabó1Beáta Bán2Csilla Józsa3László Rózsa4Attila Zsolnai5István Anton6 Association of Hungarian Limousin and Blonde d’Aquitaine Breeders, Lőportár u. 16., Budapest, 1134, Hungary Department of Animal Sciences, Széchenyi István University, Vár tér 2., Mosonmagyaróvár, 9200, Hungary National Foodchain Safety Office, Tábornok u. 2., Budapest, 1143, Hungary National Foodchain Safety Office, Tábornok u. 2., Budapest, 1143, Hungary NARIC-Research Institute for Animal Breeding Nutrition and Meat Science, Gesztenyés u. 1., Herceghalom, 2053, Hungary NARIC-Research Institute for Animal Breeding Nutrition and Meat Science, Gesztenyés u. 1., Herceghalom, 2053, Hungary NARIC-Research Institute for Animal Breeding Nutrition and Meat Science, Gesztenyés u. 1., Herceghalom, 2053, HungaryObjective This study was conducted to investigate basic information on genetic structure and characteristics of Limousin population in Hungary. Obtained results will be taken into consideration when adopting the new breeding strategy by the Association of Hungarian Limousin and Blonde d’Aquitaine Breeders (AHLBB). Methods Genetic diversity and phylogenetic relationship of 3,443 Limousin cattle from 16 different herds were investigated by performing genotyping using 18 microsatellite markers. Amplified DNA was genotyped using an automated genetic analyzer. Results Mean of effective alleles (ne) of the populations was 3.77. Population C had the lowest number of effective alleles (3.01) and the lowest inbreeding coefficient (FIS) value (−0.15). Principal component analysis of estimated genetic distance (FST) values (p<0.000) revealed two herds (C and E) distinct from the majority of other Limousin herds. The pairwise FST values of population C compared to the others (0.066 to 0.120) fell into the range of moderate genetic distance: 0.050 to 0.150, while population E displayed also moderate genetic distance (FST values in range 0.052 to 0.064) but only to six populations (G, H, J, L, N, and P). FST(C-E) was 0.148, all other pairs -excluding C and E herds- displayed low genetic distance (FST<0.049). Population D, F, I, J, K, L, N, O, and P carried private alleles, which alleles belonged to 1.1% of the individuals. Most probable number of clusters (K) were 2 and 7 determined by Structure and BAPS software. Conclusion This study showed useful genetic diversity and phylogenetic relationship data that can be utilized for the development of a new breeding strategy by AHLBB. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Limousins.http://www.ajas.info/upload/pdf/ajas-18-0164.pdfLimousin CattleMicrosatelliteGenetic DiversityGenetic Information |
| spellingShingle | Márton Szűcs Ferenc Szabó Beáta Bán Csilla Józsa László Rózsa Attila Zsolnai István Anton Assessment of genetic diversity and phylogenetic relationship of Limousin herds in Hungary using microsatellite markers Asian-Australasian Journal of Animal Sciences Limousin Cattle Microsatellite Genetic Diversity Genetic Information |
| title | Assessment of genetic diversity and phylogenetic relationship of Limousin herds in Hungary using microsatellite markers |
| title_full | Assessment of genetic diversity and phylogenetic relationship of Limousin herds in Hungary using microsatellite markers |
| title_fullStr | Assessment of genetic diversity and phylogenetic relationship of Limousin herds in Hungary using microsatellite markers |
| title_full_unstemmed | Assessment of genetic diversity and phylogenetic relationship of Limousin herds in Hungary using microsatellite markers |
| title_short | Assessment of genetic diversity and phylogenetic relationship of Limousin herds in Hungary using microsatellite markers |
| title_sort | assessment of genetic diversity and phylogenetic relationship of limousin herds in hungary using microsatellite markers |
| topic | Limousin Cattle Microsatellite Genetic Diversity Genetic Information |
| url | http://www.ajas.info/upload/pdf/ajas-18-0164.pdf |
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