Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection Volume
The aim of this study was to quantitatively define the main measurable technical parameters for the intramuscular transplantation of myogenic cells in primates. Myoblasts transduced with the gene coding for β-galactosidase were injected into the skeletal muscles of 15 monkeys. The following paramete...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2014-02-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X661337 |
| _version_ | 1818385626558365696 |
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| author | Daniel Skuk M.D. Marlyne Goulet Jacques P. Tremblay |
| author_facet | Daniel Skuk M.D. Marlyne Goulet Jacques P. Tremblay |
| author_sort | Daniel Skuk M.D. |
| collection | DOAJ |
| description | The aim of this study was to quantitatively define the main measurable technical parameters for the intramuscular transplantation of myogenic cells in primates. Myoblasts transduced with the gene coding for β-galactosidase were injected into the skeletal muscles of 15 monkeys. The following parameters were studied: needle size, number of cells per injection, and volume of cell suspension per injection. Monkeys were immunosuppressed with tacrolimus. The cell-injected sites were biopsied 1 or 2 months later. Biopsies were examined histo logically to assess the myoblast engraftment and the muscle structure. The conclusions were as follows: ( 1 ) Needles should be thin enough to avoid important tissue damage and allow muscle regeneration as satisfactory as possible. Among those tested, 27G should be the choice if the length is consistent with depth of injection. ( 2 ) At least 100,000 cells should be delivered per centimeter of needle trajectory. ( 3 ) The smallest volumes of cell suspension per injection should be used. In this study, 1 μl/cm of injection trajectory was sufficient. In principle, these parameters apply to muscles in which no damage occurred other than the injections. |
| first_indexed | 2024-12-14T03:41:09Z |
| format | Article |
| id | doaj.art-690f88701fc64e94b253a24b29d98fe7 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-14T03:41:09Z |
| publishDate | 2014-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-690f88701fc64e94b253a24b29d98fe72022-12-21T23:18:28ZengSAGE PublishingCell Transplantation0963-68971555-38922014-02-012310.3727/096368912X661337Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection VolumeDaniel Skuk M.D.0Marlyne Goulet1Jacques P. Tremblay2Neurosciences Division-Human Genetics, CHUQ Research Center-CHUL, Quebec, QC, CanadaNeurosciences Division-Human Genetics, CHUQ Research Center-CHUL, Quebec, QC, CanadaNeurosciences Division-Human Genetics, CHUQ Research Center-CHUL, Quebec, QC, CanadaThe aim of this study was to quantitatively define the main measurable technical parameters for the intramuscular transplantation of myogenic cells in primates. Myoblasts transduced with the gene coding for β-galactosidase were injected into the skeletal muscles of 15 monkeys. The following parameters were studied: needle size, number of cells per injection, and volume of cell suspension per injection. Monkeys were immunosuppressed with tacrolimus. The cell-injected sites were biopsied 1 or 2 months later. Biopsies were examined histo logically to assess the myoblast engraftment and the muscle structure. The conclusions were as follows: ( 1 ) Needles should be thin enough to avoid important tissue damage and allow muscle regeneration as satisfactory as possible. Among those tested, 27G should be the choice if the length is consistent with depth of injection. ( 2 ) At least 100,000 cells should be delivered per centimeter of needle trajectory. ( 3 ) The smallest volumes of cell suspension per injection should be used. In this study, 1 μl/cm of injection trajectory was sufficient. In principle, these parameters apply to muscles in which no damage occurred other than the injections.https://doi.org/10.3727/096368912X661337 |
| spellingShingle | Daniel Skuk M.D. Marlyne Goulet Jacques P. Tremblay Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection Volume Cell Transplantation |
| title | Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection Volume |
| title_full | Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection Volume |
| title_fullStr | Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection Volume |
| title_full_unstemmed | Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection Volume |
| title_short | Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection Volume |
| title_sort | intramuscular transplantation of myogenic cells in primates importance of needle size cell number and injection volume |
| url | https://doi.org/10.3727/096368912X661337 |
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