Natural Bis-Benzylisoquinoline Alkaloids-Tetrandrine, Fangchinoline, and Cepharanthine, Inhibit Human Coronavirus OC43 Infection of MRC-5 Human Lung Cells
<i>Stephania </i><i>tetrandra</i> and other related species of <i>Menispermaceae</i> are the major sources of the bis-benzylisoquinoline alkaloids tetrandrine (TET), fangchinoline (FAN), and cepharanthine (CEP). Although the pharmacological properties of these com...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/11/696 |
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| author | Dong Eon Kim Jung Sun Min Min Seong Jang Jun Young Lee Young Sup Shin Jong Hwan Song Hyoung Rae Kim Seungtaek Kim Young-Hee Jin Sunoh Kwon |
| author_facet | Dong Eon Kim Jung Sun Min Min Seong Jang Jun Young Lee Young Sup Shin Jong Hwan Song Hyoung Rae Kim Seungtaek Kim Young-Hee Jin Sunoh Kwon |
| author_sort | Dong Eon Kim |
| collection | DOAJ |
| description | <i>Stephania </i><i>tetrandra</i> and other related species of <i>Menispermaceae</i> are the major sources of the bis-benzylisoquinoline alkaloids tetrandrine (TET), fangchinoline (FAN), and cepharanthine (CEP). Although the pharmacological properties of these compounds include anticancer and anti-inflammatory activities, the antiviral effects of these compounds against human coronavirus (HCoV) remain unclear. Hence, the aims of the current study were to assess the antiviral activities of TET, FAN, and CEP and to elucidate the underlying mechanisms in HCoV-OC43-infected MRC-5 human lung cells. These compounds significantly inhibited virus-induced cell death at the early stage of virus infection. TET, FAN, and CEP treatment dramatically suppressed the replication of HCoV-OC43 as well as inhibited viral S and N protein expression. The virus-induced host response was reduced by compound treatment as compared with the vehicle control. Taken together, these findings demonstrate that TET, FAN, and CEP are potential natural antiviral agents for the prevention and treatment of HCoV-OC43 infection. |
| first_indexed | 2024-12-11T14:34:13Z |
| format | Article |
| id | doaj.art-69120e6e8b364d059be32f3f2763ccac |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-11T14:34:13Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-69120e6e8b364d059be32f3f2763ccac2022-12-22T01:02:17ZengMDPI AGBiomolecules2218-273X2019-11-0191169610.3390/biom9110696biom9110696Natural Bis-Benzylisoquinoline Alkaloids-Tetrandrine, Fangchinoline, and Cepharanthine, Inhibit Human Coronavirus OC43 Infection of MRC-5 Human Lung CellsDong Eon Kim0Jung Sun Min1Min Seong Jang2Jun Young Lee3Young Sup Shin4Jong Hwan Song5Hyoung Rae Kim6Seungtaek Kim7Young-Hee Jin8Sunoh Kwon9Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaZoonotic Virus Laboratory, Institute Pasteur Korea, Seongnam 13488, KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea<i>Stephania </i><i>tetrandra</i> and other related species of <i>Menispermaceae</i> are the major sources of the bis-benzylisoquinoline alkaloids tetrandrine (TET), fangchinoline (FAN), and cepharanthine (CEP). Although the pharmacological properties of these compounds include anticancer and anti-inflammatory activities, the antiviral effects of these compounds against human coronavirus (HCoV) remain unclear. Hence, the aims of the current study were to assess the antiviral activities of TET, FAN, and CEP and to elucidate the underlying mechanisms in HCoV-OC43-infected MRC-5 human lung cells. These compounds significantly inhibited virus-induced cell death at the early stage of virus infection. TET, FAN, and CEP treatment dramatically suppressed the replication of HCoV-OC43 as well as inhibited viral S and N protein expression. The virus-induced host response was reduced by compound treatment as compared with the vehicle control. Taken together, these findings demonstrate that TET, FAN, and CEP are potential natural antiviral agents for the prevention and treatment of HCoV-OC43 infection.https://www.mdpi.com/2218-273X/9/11/696bis-benzylisoquinoline alkaloidstetrandrinefangchinolinecepharanthinehuman coronavirus strain oc43mrc-5 human lung cellantiviral effect |
| spellingShingle | Dong Eon Kim Jung Sun Min Min Seong Jang Jun Young Lee Young Sup Shin Jong Hwan Song Hyoung Rae Kim Seungtaek Kim Young-Hee Jin Sunoh Kwon Natural Bis-Benzylisoquinoline Alkaloids-Tetrandrine, Fangchinoline, and Cepharanthine, Inhibit Human Coronavirus OC43 Infection of MRC-5 Human Lung Cells Biomolecules bis-benzylisoquinoline alkaloids tetrandrine fangchinoline cepharanthine human coronavirus strain oc43 mrc-5 human lung cell antiviral effect |
| title | Natural Bis-Benzylisoquinoline Alkaloids-Tetrandrine, Fangchinoline, and Cepharanthine, Inhibit Human Coronavirus OC43 Infection of MRC-5 Human Lung Cells |
| title_full | Natural Bis-Benzylisoquinoline Alkaloids-Tetrandrine, Fangchinoline, and Cepharanthine, Inhibit Human Coronavirus OC43 Infection of MRC-5 Human Lung Cells |
| title_fullStr | Natural Bis-Benzylisoquinoline Alkaloids-Tetrandrine, Fangchinoline, and Cepharanthine, Inhibit Human Coronavirus OC43 Infection of MRC-5 Human Lung Cells |
| title_full_unstemmed | Natural Bis-Benzylisoquinoline Alkaloids-Tetrandrine, Fangchinoline, and Cepharanthine, Inhibit Human Coronavirus OC43 Infection of MRC-5 Human Lung Cells |
| title_short | Natural Bis-Benzylisoquinoline Alkaloids-Tetrandrine, Fangchinoline, and Cepharanthine, Inhibit Human Coronavirus OC43 Infection of MRC-5 Human Lung Cells |
| title_sort | natural bis benzylisoquinoline alkaloids tetrandrine fangchinoline and cepharanthine inhibit human coronavirus oc43 infection of mrc 5 human lung cells |
| topic | bis-benzylisoquinoline alkaloids tetrandrine fangchinoline cepharanthine human coronavirus strain oc43 mrc-5 human lung cell antiviral effect |
| url | https://www.mdpi.com/2218-273X/9/11/696 |
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